Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells
Plant-derived nanovesicles emerge as a promising alternative to mammalian-derived exosomes with distinct advantages, including lower immunogenicity, enhanced bioavailability, and the presence of bioactive plant metabolites. They have been shown to cross biological barriers, delivering therapeutic mo...
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| Format: | Article |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1617478/full |
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| author | Clarissa Zanotti Clarissa Zanotti Simona Arena Sabrina De Pascale Valentina Ciaravolo Rosalia Ferracane Antonio Dario Troise Chiara Pontecorvi Francesca Pacello Chiara Niespolo Angelo Gismondi Andrea Scaloni Mauro Marra |
| author_facet | Clarissa Zanotti Clarissa Zanotti Simona Arena Sabrina De Pascale Valentina Ciaravolo Rosalia Ferracane Antonio Dario Troise Chiara Pontecorvi Francesca Pacello Chiara Niespolo Angelo Gismondi Andrea Scaloni Mauro Marra |
| author_sort | Clarissa Zanotti |
| collection | DOAJ |
| description | Plant-derived nanovesicles emerge as a promising alternative to mammalian-derived exosomes with distinct advantages, including lower immunogenicity, enhanced bioavailability, and the presence of bioactive plant metabolites. They have been shown to cross biological barriers, delivering therapeutic molecules that modulate gene expression, inflammation, oxidative stress, and cancer-related pathways. However, challenges remain that limit applicative use, including poor knowledge of their interactions with mammalian host cells and primarily the lack of a cost-effective and scalable method to obtain highly purified plant-derived nanovesicles. To address these limitations, we have developed an advanced purification platform that integrates ultrafiltration with anion exchange chromatography in a fast protein liquid chromatography system. This approach was validated using it in the isolation of plant-derived nanovesicles from Brassica oleracea L. seedlings, resulting in highly purified and concentrated preparations. Comprehensive molecular analyses, including proteomics, lipidomics, metabolomics, and miRNA profiling, characterized the nature of the purified plant-derived nanovesicles. Furthermore, their wound healing and anti-inflammatory properties were demonstrated in vitro and correlated to the potential biological activities of cargo miRNAs species by bioinformatics, highlighting the potential in nanomedicine of anion exchange-purified brassica nanovesicles. This study provides a scalable and efficient purification strategy, which might pave the way for broader applications of plant-derived nanovesicles in the clinical, nutraceutical and pharmaceutical fields. |
| format | Article |
| id | doaj-art-712fca2751ea43a8a73ec6b3c7d9fc70 |
| institution | DOAJ |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-712fca2751ea43a8a73ec6b3c7d9fc702025-08-20T03:09:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-07-011310.3389/fbioe.2025.16174781617478Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cellsClarissa Zanotti0Clarissa Zanotti1Simona Arena2Sabrina De Pascale3Valentina Ciaravolo4Rosalia Ferracane5Antonio Dario Troise6Chiara Pontecorvi7Francesca Pacello8Chiara Niespolo9Angelo Gismondi10Andrea Scaloni11Mauro Marra12Department of Biology, University of Rome Tor Vergata, Rome, ItalyPhD Program in Cellular and Molecular Biology, Department of Biology, University of Rome “Tor Vergata”, Rome, ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyArterra Bioscience SpA, Naples, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, ItalyDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyPlant-derived nanovesicles emerge as a promising alternative to mammalian-derived exosomes with distinct advantages, including lower immunogenicity, enhanced bioavailability, and the presence of bioactive plant metabolites. They have been shown to cross biological barriers, delivering therapeutic molecules that modulate gene expression, inflammation, oxidative stress, and cancer-related pathways. However, challenges remain that limit applicative use, including poor knowledge of their interactions with mammalian host cells and primarily the lack of a cost-effective and scalable method to obtain highly purified plant-derived nanovesicles. To address these limitations, we have developed an advanced purification platform that integrates ultrafiltration with anion exchange chromatography in a fast protein liquid chromatography system. This approach was validated using it in the isolation of plant-derived nanovesicles from Brassica oleracea L. seedlings, resulting in highly purified and concentrated preparations. Comprehensive molecular analyses, including proteomics, lipidomics, metabolomics, and miRNA profiling, characterized the nature of the purified plant-derived nanovesicles. Furthermore, their wound healing and anti-inflammatory properties were demonstrated in vitro and correlated to the potential biological activities of cargo miRNAs species by bioinformatics, highlighting the potential in nanomedicine of anion exchange-purified brassica nanovesicles. This study provides a scalable and efficient purification strategy, which might pave the way for broader applications of plant-derived nanovesicles in the clinical, nutraceutical and pharmaceutical fields.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1617478/fullplant-derived nanovesiclesBrassica oleracea L.proteomicslipidomicsmiRNomicswound healing |
| spellingShingle | Clarissa Zanotti Clarissa Zanotti Simona Arena Sabrina De Pascale Valentina Ciaravolo Rosalia Ferracane Antonio Dario Troise Chiara Pontecorvi Francesca Pacello Chiara Niespolo Angelo Gismondi Andrea Scaloni Mauro Marra Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells Frontiers in Bioengineering and Biotechnology plant-derived nanovesicles Brassica oleracea L. proteomics lipidomics miRNomics wound healing |
| title | Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells |
| title_full | Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells |
| title_fullStr | Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells |
| title_full_unstemmed | Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells |
| title_short | Anion exchange chromatography-based purification of plant-derived nanovesicles from Brassica oleracea L.: molecular profiling and bioactivity in human cells |
| title_sort | anion exchange chromatography based purification of plant derived nanovesicles from brassica oleracea l molecular profiling and bioactivity in human cells |
| topic | plant-derived nanovesicles Brassica oleracea L. proteomics lipidomics miRNomics wound healing |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1617478/full |
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