A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer
In the existence of appropriate amount of disodium ethylenediaminetetraacetate (EDTA), precipitation would not occur in seawater and other natural waters even if the sample solution was adjusted to strong basicity, and the NH3-OPA-sulfite reaction at the optimal pH range could be used to determine a...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/728068 |
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| author | Hongzhi Hu Ying Liang Shuo Li Qing Guo Chancui Wu |
| author_facet | Hongzhi Hu Ying Liang Shuo Li Qing Guo Chancui Wu |
| author_sort | Hongzhi Hu |
| collection | DOAJ |
| description | In the existence of appropriate amount of disodium ethylenediaminetetraacetate (EDTA), precipitation would not occur in seawater and other natural waters even if the sample solution was adjusted to strong basicity, and the NH3-OPA-sulfite reaction at the optimal pH range could be used to determine ammonium in natural waters. Based on this, a modified o-phthalaldehyde fluorometric analytical method has been established to determine ultratrace ammonium in natural waters. Experimental parameters, including reagent concentration, pH, reaction time, and effect of EDTA, were optimized throughout the experiments based on univariate experimental design. The results showed that the optimal pH range was between 10.80 and 11.70. EDTA did not obviously affect the fluorometric intensity. The linearity range of the proposed method was 0.032–0.500 µmol/L, 0.250–3.00 µmol/L, and 1.00–20.0 µmol/L at the excitation/emission slit of 3 nm/5 nm, 3 nm/3 nm, and 1.5 nm/1.5 nm, respectively. The method detection limit was 0.0099 µmol/L. Compared to the classical OPA method, the proposed method had the advantage of being more sensitive and could quantify ultratrace ammonium without enrichment. |
| format | Article |
| id | doaj-art-712a06f13d1d42ed8cc07a19c889fe2d |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-712a06f13d1d42ed8cc07a19c889fe2d2025-02-03T07:23:55ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/728068728068A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as BufferHongzhi Hu0Ying Liang1Shuo Li2Qing Guo3Chancui Wu4School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaIn the existence of appropriate amount of disodium ethylenediaminetetraacetate (EDTA), precipitation would not occur in seawater and other natural waters even if the sample solution was adjusted to strong basicity, and the NH3-OPA-sulfite reaction at the optimal pH range could be used to determine ammonium in natural waters. Based on this, a modified o-phthalaldehyde fluorometric analytical method has been established to determine ultratrace ammonium in natural waters. Experimental parameters, including reagent concentration, pH, reaction time, and effect of EDTA, were optimized throughout the experiments based on univariate experimental design. The results showed that the optimal pH range was between 10.80 and 11.70. EDTA did not obviously affect the fluorometric intensity. The linearity range of the proposed method was 0.032–0.500 µmol/L, 0.250–3.00 µmol/L, and 1.00–20.0 µmol/L at the excitation/emission slit of 3 nm/5 nm, 3 nm/3 nm, and 1.5 nm/1.5 nm, respectively. The method detection limit was 0.0099 µmol/L. Compared to the classical OPA method, the proposed method had the advantage of being more sensitive and could quantify ultratrace ammonium without enrichment.http://dx.doi.org/10.1155/2014/728068 |
| spellingShingle | Hongzhi Hu Ying Liang Shuo Li Qing Guo Chancui Wu A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer Journal of Analytical Methods in Chemistry |
| title | A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer |
| title_full | A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer |
| title_fullStr | A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer |
| title_full_unstemmed | A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer |
| title_short | A Modified o-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer |
| title_sort | modified o phthalaldehyde fluorometric analytical method for ultratrace ammonium in natural waters using edta naoh as buffer |
| url | http://dx.doi.org/10.1155/2014/728068 |
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