The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats

Homeostasis preservation is essential for animal survival, and any event that causes a disturbance in homeostasis is defined as a stressor. Here, we aimed to evaluate the effect of scratch brushes and stages as an environmental enrichment to alleviate stress in dairy goats. Twenty-four mixed-breed g...

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Main Authors: Yossi Wein, Ofri Vaidenfeld, Chris Sabastian, Enav Bar Shira, Sameer J. Mabjeesh, Haim Tagari, Aharon Friedman
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Biology
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Online Access:https://www.mdpi.com/2079-7737/13/11/859
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author Yossi Wein
Ofri Vaidenfeld
Chris Sabastian
Enav Bar Shira
Sameer J. Mabjeesh
Haim Tagari
Aharon Friedman
author_facet Yossi Wein
Ofri Vaidenfeld
Chris Sabastian
Enav Bar Shira
Sameer J. Mabjeesh
Haim Tagari
Aharon Friedman
author_sort Yossi Wein
collection DOAJ
description Homeostasis preservation is essential for animal survival, and any event that causes a disturbance in homeostasis is defined as a stressor. Here, we aimed to evaluate the effect of scratch brushes and stages as an environmental enrichment to alleviate stress in dairy goats. Twenty-four mixed-breed goats were divided into two groups according to common physiological conditions in breeding farms: milking and dry (milk-producing and non-milk-producing, respectively). Ten days after exposure to environmental enrichment treatment or not (control), blood was sampled. Following the enrichment, we observed a reduction in reactive oxidative stress metabolites, advanced glycation end products (AGEs), and their binding protein (transferrin) in the dry goats, as determined by an ELISA. In contrast, no change in AGEs, along with an increase in transferrin levels, was observed in the milking goats. Moreover, oxytocin levels decreased in the dry and increased in the milking goats, while serotonin levels increased in the dry and remained unchanged in the milking goats. Additionally, gene expression of the cytokines, IL-6 and IL-1ß, and anti-oxidative proteins, lysozyme and transferrin (in peripheral blood leukocytes), as determined by qPCR, presented the same pattern: down-regulation in the dry or up-regulation in the milking goats. In conclusion, a reliable methodology was developed for measuring husbandry stress in goats and to improve dairy goats’ husbandry practice. Current environmental enrichment produced different responsiveness in goats correlated to their physiological status: beneficial effect in dry goats, detrimental effect in milking goats.
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spelling doaj-art-7127dbda8b4a458288d9dcbf318c01da2025-08-20T01:53:40ZengMDPI AGBiology2079-77372024-10-01131185910.3390/biology13110859The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy GoatsYossi Wein0Ofri Vaidenfeld1Chris Sabastian2Enav Bar Shira3Sameer J. Mabjeesh4Haim Tagari5Aharon Friedman6Department of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelDepartment of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelDepartment of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelDepartment of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelDepartment of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelDepartment of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelDepartment of Animal Sciences, R.H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 7610001, IsraelHomeostasis preservation is essential for animal survival, and any event that causes a disturbance in homeostasis is defined as a stressor. Here, we aimed to evaluate the effect of scratch brushes and stages as an environmental enrichment to alleviate stress in dairy goats. Twenty-four mixed-breed goats were divided into two groups according to common physiological conditions in breeding farms: milking and dry (milk-producing and non-milk-producing, respectively). Ten days after exposure to environmental enrichment treatment or not (control), blood was sampled. Following the enrichment, we observed a reduction in reactive oxidative stress metabolites, advanced glycation end products (AGEs), and their binding protein (transferrin) in the dry goats, as determined by an ELISA. In contrast, no change in AGEs, along with an increase in transferrin levels, was observed in the milking goats. Moreover, oxytocin levels decreased in the dry and increased in the milking goats, while serotonin levels increased in the dry and remained unchanged in the milking goats. Additionally, gene expression of the cytokines, IL-6 and IL-1ß, and anti-oxidative proteins, lysozyme and transferrin (in peripheral blood leukocytes), as determined by qPCR, presented the same pattern: down-regulation in the dry or up-regulation in the milking goats. In conclusion, a reliable methodology was developed for measuring husbandry stress in goats and to improve dairy goats’ husbandry practice. Current environmental enrichment produced different responsiveness in goats correlated to their physiological status: beneficial effect in dry goats, detrimental effect in milking goats.https://www.mdpi.com/2079-7737/13/11/859dairy goatshusbandry stressenvironmental enrichmentadvanced glycation end productsperipheral blood leukocytestransferrin
spellingShingle Yossi Wein
Ofri Vaidenfeld
Chris Sabastian
Enav Bar Shira
Sameer J. Mabjeesh
Haim Tagari
Aharon Friedman
The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats
Biology
dairy goats
husbandry stress
environmental enrichment
advanced glycation end products
peripheral blood leukocytes
transferrin
title The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats
title_full The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats
title_fullStr The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats
title_full_unstemmed The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats
title_short The Effect of Environmental Enrichment on Selected Physiological and Immunological Stress-Related Markers in Dairy Goats
title_sort effect of environmental enrichment on selected physiological and immunological stress related markers in dairy goats
topic dairy goats
husbandry stress
environmental enrichment
advanced glycation end products
peripheral blood leukocytes
transferrin
url https://www.mdpi.com/2079-7737/13/11/859
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