Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics
Mesenchymal progenitors within bone marrow have multiple differentiation potential and play an essential role in the maintenance of adult skeleton homeostasis. Mesenchymal progenitors located in bone regions other than the bone marrow also display bone-forming properties. However, owing to the diffe...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5037578 |
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| author | Weiguang Lu Bo Gao Jing Fan Pengzhen Cheng Yaqian Hu Qiang Jie Zhuojing Luo Liu Yang |
| author_facet | Weiguang Lu Bo Gao Jing Fan Pengzhen Cheng Yaqian Hu Qiang Jie Zhuojing Luo Liu Yang |
| author_sort | Weiguang Lu |
| collection | DOAJ |
| description | Mesenchymal progenitors within bone marrow have multiple differentiation potential and play an essential role in the maintenance of adult skeleton homeostasis. Mesenchymal progenitors located in bone regions other than the bone marrow also display bone-forming properties. However, owing to the differences in each distinct microenvironment, the mesenchymal characteristics of skeletal progenitor cells within different regions of long bones may show some differences. In order to clearly elucidate these differences, we performed a comparative study on mesenchymal progenitors from different regions of long bones. Here, we isolated mesenchymal progenitors from the periosteum, endosteum, and bone marrow of rat long bones. The three groups exhibited similar cellular morphologies and expressed the typical surface markers associated with mesenchymal stem cells. Interestingly, after cell proliferation assays and bidirectional differentiation analysis, periosteal mesenchymal progenitors showed a higher proliferative ability and adipogenic differentiation potential. In contrast, endosteal mesenchymal progenitors were more prone to osteogenic differentiation. Using in vitro osteoclast culture systems, conditioned media from different mesenchymal progenitor cultures were used to induce osteoclastic differentiation. Osteoclast formation was found to be significantly promoted by the secretion of RANKL and IL-6 by endosteal progenitors. Overall, our results provide strong evidence for the importance of selecting the appropriate source of skeletal progenitors for applications in future skeleton regeneration therapies. |
| format | Article |
| id | doaj-art-71278b4ac4f148baa2e68b28e3315a15 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-71278b4ac4f148baa2e68b28e3315a152025-02-03T05:43:57ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/50375785037578Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse CharacteristicsWeiguang Lu0Bo Gao1Jing Fan2Pengzhen Cheng3Yaqian Hu4Qiang Jie5Zhuojing Luo6Liu Yang7Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaInstitute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaInstitute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaInstitute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaInstitute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaDepartment of Orthopedic Surgery, Honghui Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, ChinaInstitute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaInstitute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, ChinaMesenchymal progenitors within bone marrow have multiple differentiation potential and play an essential role in the maintenance of adult skeleton homeostasis. Mesenchymal progenitors located in bone regions other than the bone marrow also display bone-forming properties. However, owing to the differences in each distinct microenvironment, the mesenchymal characteristics of skeletal progenitor cells within different regions of long bones may show some differences. In order to clearly elucidate these differences, we performed a comparative study on mesenchymal progenitors from different regions of long bones. Here, we isolated mesenchymal progenitors from the periosteum, endosteum, and bone marrow of rat long bones. The three groups exhibited similar cellular morphologies and expressed the typical surface markers associated with mesenchymal stem cells. Interestingly, after cell proliferation assays and bidirectional differentiation analysis, periosteal mesenchymal progenitors showed a higher proliferative ability and adipogenic differentiation potential. In contrast, endosteal mesenchymal progenitors were more prone to osteogenic differentiation. Using in vitro osteoclast culture systems, conditioned media from different mesenchymal progenitor cultures were used to induce osteoclastic differentiation. Osteoclast formation was found to be significantly promoted by the secretion of RANKL and IL-6 by endosteal progenitors. Overall, our results provide strong evidence for the importance of selecting the appropriate source of skeletal progenitors for applications in future skeleton regeneration therapies.http://dx.doi.org/10.1155/2019/5037578 |
| spellingShingle | Weiguang Lu Bo Gao Jing Fan Pengzhen Cheng Yaqian Hu Qiang Jie Zhuojing Luo Liu Yang Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics Stem Cells International |
| title | Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics |
| title_full | Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics |
| title_fullStr | Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics |
| title_full_unstemmed | Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics |
| title_short | Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics |
| title_sort | mesenchymal progenitors derived from different locations in long bones display diverse characteristics |
| url | http://dx.doi.org/10.1155/2019/5037578 |
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