DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides
Herein, a dissipative system tailored for the controlled loading and release of CpG oligodeoxynucleotides (CpG ODNs), known for their pharmacological immunostimulatory properties, is reported. The approach involves multiple cycles of deactivation and activation of the CpG ODNs via its hybridization...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-11-01
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| Series: | Advanced NanoBiomed Research |
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| Online Access: | https://doi.org/10.1002/anbr.202400082 |
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| author | Aman Ishaqat Xiaofeng Zhang Andreas Herrmann |
| author_facet | Aman Ishaqat Xiaofeng Zhang Andreas Herrmann |
| author_sort | Aman Ishaqat |
| collection | DOAJ |
| description | Herein, a dissipative system tailored for the controlled loading and release of CpG oligodeoxynucleotides (CpG ODNs), known for their pharmacological immunostimulatory properties, is reported. The approach involves multiple cycles of deactivation and activation of the CpG ODNs via its hybridization with a complementary fuel strand, followed by its selective release mediated by the enzymatic activity of T7 exonuclease. The autonomous and temporal behavior of this dissipative system can be tuned by three factors: the design of the fuel strand and its concentration that governs the kinetics of the forward hybridization reaction, as well as the concentration of T7 exonuclease, which regulates the backward energy dissipation reaction. Furthermore, the enzyme's tolerance toward waste accumulation is demonstrated, and the system's robust performance when utilizing various fuel strands in alternating fashion is showcased. The findings underscore the potential of this approach for precise and programmable delivery of therapeutic nucleic acids in multiple cycles, with implications for enhancing immunotherapeutic strategies in which controlled kinetics of the nucleic acid is highly desired. |
| format | Article |
| id | doaj-art-712314302cbd4b2181ff3a026ceead9f |
| institution | OA Journals |
| issn | 2699-9307 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj-art-712314302cbd4b2181ff3a026ceead9f2025-08-20T02:12:41ZengWiley-VCHAdvanced NanoBiomed Research2699-93072024-11-01411n/an/a10.1002/anbr.202400082DNA Dissipative System for Controlled Release of Immunostimulatory CpG OligodeoxynucleotidesAman Ishaqat0Xiaofeng Zhang1Andreas Herrmann2Institute of Technical and Macromolecular Chemistry RWTH Aachen University Worringerweg 2 52074 Aachen GermanyInstitute of Technical and Macromolecular Chemistry RWTH Aachen University Worringerweg 2 52074 Aachen GermanyInstitute of Technical and Macromolecular Chemistry RWTH Aachen University Worringerweg 2 52074 Aachen GermanyHerein, a dissipative system tailored for the controlled loading and release of CpG oligodeoxynucleotides (CpG ODNs), known for their pharmacological immunostimulatory properties, is reported. The approach involves multiple cycles of deactivation and activation of the CpG ODNs via its hybridization with a complementary fuel strand, followed by its selective release mediated by the enzymatic activity of T7 exonuclease. The autonomous and temporal behavior of this dissipative system can be tuned by three factors: the design of the fuel strand and its concentration that governs the kinetics of the forward hybridization reaction, as well as the concentration of T7 exonuclease, which regulates the backward energy dissipation reaction. Furthermore, the enzyme's tolerance toward waste accumulation is demonstrated, and the system's robust performance when utilizing various fuel strands in alternating fashion is showcased. The findings underscore the potential of this approach for precise and programmable delivery of therapeutic nucleic acids in multiple cycles, with implications for enhancing immunotherapeutic strategies in which controlled kinetics of the nucleic acid is highly desired.https://doi.org/10.1002/anbr.202400082CpG oligodeoxynucleotidesdissipative systemsDNA |
| spellingShingle | Aman Ishaqat Xiaofeng Zhang Andreas Herrmann DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides Advanced NanoBiomed Research CpG oligodeoxynucleotides dissipative systems DNA |
| title | DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides |
| title_full | DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides |
| title_fullStr | DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides |
| title_full_unstemmed | DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides |
| title_short | DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides |
| title_sort | dna dissipative system for controlled release of immunostimulatory cpg oligodeoxynucleotides |
| topic | CpG oligodeoxynucleotides dissipative systems DNA |
| url | https://doi.org/10.1002/anbr.202400082 |
| work_keys_str_mv | AT amanishaqat dnadissipativesystemforcontrolledreleaseofimmunostimulatorycpgoligodeoxynucleotides AT xiaofengzhang dnadissipativesystemforcontrolledreleaseofimmunostimulatorycpgoligodeoxynucleotides AT andreasherrmann dnadissipativesystemforcontrolledreleaseofimmunostimulatorycpgoligodeoxynucleotides |