Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β

Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation....

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Main Authors: Mengmeng Xu, Mengmeng Huang, Guodong WANG, Jingzhe HAN, Xinxin NIU, Jinze HAN, Hangbo YU, Yulong ZHANG, Runhang LIU, Ziwen WU, Hongyu Cui, Yanping Zhang, Suyan Wang, Yongzhen Liu, Yuntong Chen, Yulu Duan, Liuan Li, Yulong Gao, Xiaole Qi
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125000446
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author Mengmeng Xu
Mengmeng Huang
Guodong WANG
Jingzhe HAN
Xinxin NIU
Jinze HAN
Hangbo YU
Yulong ZHANG
Runhang LIU
Ziwen WU
Hongyu Cui
Yanping Zhang
Suyan Wang
Yongzhen Liu
Yuntong Chen
Yulu Duan
Liuan Li
Yulong Gao
Xiaole Qi
author_facet Mengmeng Xu
Mengmeng Huang
Guodong WANG
Jingzhe HAN
Xinxin NIU
Jinze HAN
Hangbo YU
Yulong ZHANG
Runhang LIU
Ziwen WU
Hongyu Cui
Yanping Zhang
Suyan Wang
Yongzhen Liu
Yuntong Chen
Yulu Duan
Liuan Li
Yulong Gao
Xiaole Qi
author_sort Mengmeng Xu
collection DOAJ
description Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1β, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1β monoclonal antibody (mAb). The secreted mAb 1E12 can recognize exogenous or endogenous chicken pro-IL-1β by Western blot and IFA techniques, and for the first time, a novel antigen epitope 13SSLSEETFY21 of chicken pro-IL-1β recognized by this mAb was identified. This study not only provides an important tool for the detection and research of chicken pro-IL-1β, but also has significant implications for understanding the antigenic structure and biochemical characteristics of chicken pro-IL-1β.
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record_format Article
series Poultry Science
spelling doaj-art-7120827830ed49beb485e03dce7493a02025-01-24T04:43:55ZengElsevierPoultry Science0032-57912025-02-011042104807Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1βMengmeng Xu0Mengmeng Huang1Guodong WANG2Jingzhe HAN3Xinxin NIU4Jinze HAN5Hangbo YU6Yulong ZHANG7Runhang LIU8Ziwen WU9Hongyu Cui10Yanping Zhang11Suyan Wang12Yongzhen Liu13Yuntong Chen14Yulu Duan15Liuan Li16Yulong Gao17Xiaole Qi18Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China; Corresponding authors at: State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China.Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, China; Corresponding authors at: State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China.Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, China; Corresponding authors at: State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China.Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1β, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1β monoclonal antibody (mAb). The secreted mAb 1E12 can recognize exogenous or endogenous chicken pro-IL-1β by Western blot and IFA techniques, and for the first time, a novel antigen epitope 13SSLSEETFY21 of chicken pro-IL-1β recognized by this mAb was identified. This study not only provides an important tool for the detection and research of chicken pro-IL-1β, but also has significant implications for understanding the antigenic structure and biochemical characteristics of chicken pro-IL-1β.http://www.sciencedirect.com/science/article/pii/S0032579125000446Pro-IL-1βChickenMonoclonal antibodyDetectionAntigenic epitope
spellingShingle Mengmeng Xu
Mengmeng Huang
Guodong WANG
Jingzhe HAN
Xinxin NIU
Jinze HAN
Hangbo YU
Yulong ZHANG
Runhang LIU
Ziwen WU
Hongyu Cui
Yanping Zhang
Suyan Wang
Yongzhen Liu
Yuntong Chen
Yulu Duan
Liuan Li
Yulong Gao
Xiaole Qi
Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β
Poultry Science
Pro-IL-1β
Chicken
Monoclonal antibody
Detection
Antigenic epitope
title Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β
title_full Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β
title_fullStr Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β
title_full_unstemmed Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β
title_short Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β
title_sort monoclonal antibody development and antigenic epitope identification of chicken pro il 1β
topic Pro-IL-1β
Chicken
Monoclonal antibody
Detection
Antigenic epitope
url http://www.sciencedirect.com/science/article/pii/S0032579125000446
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