A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila
Parkinson's disease is a common neurodegenerative disorder with the pathology of α-synuclein aggregation in Lewy bodies. Currently, there is no available therapy that arrests the progression of the disease. Therefore, the need of animal models to follow α-synuclein aggregation is crucial. Droso...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Parkinson's Disease |
| Online Access: | http://dx.doi.org/10.1155/2012/697564 |
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| author | Ronit Shaltiel-Karyo Dan Davidi Yotam Menuchin Moran Frenkel-Pinter Mira Marcus-Kalish John Ringo Ehud Gazit Daniel Segal |
| author_facet | Ronit Shaltiel-Karyo Dan Davidi Yotam Menuchin Moran Frenkel-Pinter Mira Marcus-Kalish John Ringo Ehud Gazit Daniel Segal |
| author_sort | Ronit Shaltiel-Karyo |
| collection | DOAJ |
| description | Parkinson's disease is a common neurodegenerative disorder with the pathology of α-synuclein aggregation in Lewy bodies. Currently, there is no available therapy that arrests the progression of the disease. Therefore, the need of animal models to follow α-synuclein aggregation is crucial. Drosophila melanogaster has been researched extensively as a good genetic model for the disease, with a cognitive phenotype of defective climbing ability. The assay for climbing ability has been demonstrated as an effective tool for screening new therapeutic agents for Parkinson's disease. However, due to the assay's many limitations, there is a clear need to develop a better behavioral test. Courtship, a stereotyped, ritualized behavior of Drosophila, involves complex motor and sensory functions in both sexes, which are controlled by large number of neurons; hence, behavior observed during courtship should be sensitive to disease processes in the nervous system. We used a series of traits commonly observed in courtship and an additional behavioral trait—nonsexual encounters—and analyzed them using a data mining tool. We found defective behavior of the Parkinson's model male flies that were tested with virgin females, visible at a much younger age than the climbing defects. We conclude that this is an improved behavioral assay for Parkinson's model flies. |
| format | Article |
| id | doaj-art-711965cae23a4f99a426882e84fba970 |
| institution | Kabale University |
| issn | 2090-8083 2042-0080 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Parkinson's Disease |
| spelling | doaj-art-711965cae23a4f99a426882e84fba9702025-08-20T03:33:57ZengWileyParkinson's Disease2090-80832042-00802012-01-01201210.1155/2012/697564697564A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model DrosophilaRonit Shaltiel-Karyo0Dan Davidi1Yotam Menuchin2Moran Frenkel-Pinter3Mira Marcus-Kalish4John Ringo5Ehud Gazit6Daniel Segal7Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, IsraelDepartment of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, IsraelDepartment of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, IsraelDepartment of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, IsraelInterdisciplinary Center for Technology Analysis & Forecasting (ICTAF), Tel Aviv University, 69978 Tel Aviv, IsraelSchool of Biology, The University of Maine, Orono, ME 04469, USADepartment of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, IsraelDepartment of Molecular Microbiology and Biotechnology, Tel Aviv University, 69978 Tel Aviv, IsraelParkinson's disease is a common neurodegenerative disorder with the pathology of α-synuclein aggregation in Lewy bodies. Currently, there is no available therapy that arrests the progression of the disease. Therefore, the need of animal models to follow α-synuclein aggregation is crucial. Drosophila melanogaster has been researched extensively as a good genetic model for the disease, with a cognitive phenotype of defective climbing ability. The assay for climbing ability has been demonstrated as an effective tool for screening new therapeutic agents for Parkinson's disease. However, due to the assay's many limitations, there is a clear need to develop a better behavioral test. Courtship, a stereotyped, ritualized behavior of Drosophila, involves complex motor and sensory functions in both sexes, which are controlled by large number of neurons; hence, behavior observed during courtship should be sensitive to disease processes in the nervous system. We used a series of traits commonly observed in courtship and an additional behavioral trait—nonsexual encounters—and analyzed them using a data mining tool. We found defective behavior of the Parkinson's model male flies that were tested with virgin females, visible at a much younger age than the climbing defects. We conclude that this is an improved behavioral assay for Parkinson's model flies.http://dx.doi.org/10.1155/2012/697564 |
| spellingShingle | Ronit Shaltiel-Karyo Dan Davidi Yotam Menuchin Moran Frenkel-Pinter Mira Marcus-Kalish John Ringo Ehud Gazit Daniel Segal A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila Parkinson's Disease |
| title | A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila |
| title_full | A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila |
| title_fullStr | A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila |
| title_full_unstemmed | A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila |
| title_short | A Novel, Sensitive Assay for Behavioral Defects in Parkinson's Disease Model Drosophila |
| title_sort | novel sensitive assay for behavioral defects in parkinson s disease model drosophila |
| url | http://dx.doi.org/10.1155/2012/697564 |
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