Diffractive remote focusing module for high-NA scanning microscopy operating at Galvo speeds
We demonstrate a remote axial scanning system for optical scanning microscopy that operates in the millisecond range over a large depth of approximately 80 times the longitudinal size of the focal spot, and that retains the focal spot size by compensating for spherical aberrations. This is achieved...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2025-01-01
|
| Series: | JPhys Photonics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2515-7647/ade1bd |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | We demonstrate a remote axial scanning system for optical scanning microscopy that operates in the millisecond range over a large depth of approximately 80 times the longitudinal size of the focal spot, and that retains the focal spot size by compensating for spherical aberrations. This is achieved by controlling the divergence of the scanning beam with a special pair of adjacent diffractive optical elements (DOEs) that form a so-called moiré lens whose focal length is controlled by a relative rotation between the two DOEs. The moiré lens system is used in reflection mode, where one of the DOEs is fixed while the second, reflecting DOE is mounted perpendicularly on the axis of a rotating Galvo stage that can achieve an angular range of ± 20 ^∘ in the 50 Hz range. The moiré lens is specially adapted to allow a large optical power change of ±13 dpt in this angular range while compensating for the spherical aberrations, which otherwise would be induced by the focus shift. The remote focusing module can be used in conjunction with any laser scanning microscopy method, such as confocal or multi-photon microscopy. We experimentally demonstrate its operation in a fast confocal profilometer. |
|---|---|
| ISSN: | 2515-7647 |