Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment
Abstract Lipo-chitooligosaccharides (LCO) and short-chain chitooligosaccharides (CO) are produced by arbuscular mycorrhizal fungi (AMF) and activate the plant symbiosis signalling pathway, which is essential for mycorrhiza formation. High-affinity LCO receptors belonging to the LysM receptor-like ki...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60717-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849402071570186240 |
|---|---|
| author | Yi Ding Zélie Lesterps Virginie Gasciolli Anne-Laure Fuchs Mégane Gaston Lauréna Medioni Annélie de-Regibus Céline Remblière Céline Vicédo Sandra Bensmihen Jean Jacques Bono Julie Cullimore Guilhem Reyt Marion Dalmais Christine Saffray Solène Mazeau Abdelhafid Bendahmane Richard Sibout Michiel Vandenbussche Jacques Rouster Tongming Wang Guanghua He Arnaud Masselin Sylvain Cottaz Sébastien Fort Benoit Lefebvre |
| author_facet | Yi Ding Zélie Lesterps Virginie Gasciolli Anne-Laure Fuchs Mégane Gaston Lauréna Medioni Annélie de-Regibus Céline Remblière Céline Vicédo Sandra Bensmihen Jean Jacques Bono Julie Cullimore Guilhem Reyt Marion Dalmais Christine Saffray Solène Mazeau Abdelhafid Bendahmane Richard Sibout Michiel Vandenbussche Jacques Rouster Tongming Wang Guanghua He Arnaud Masselin Sylvain Cottaz Sébastien Fort Benoit Lefebvre |
| author_sort | Yi Ding |
| collection | DOAJ |
| description | Abstract Lipo-chitooligosaccharides (LCO) and short-chain chitooligosaccharides (CO) are produced by arbuscular mycorrhizal fungi (AMF) and activate the plant symbiosis signalling pathway, which is essential for mycorrhiza formation. High-affinity LCO receptors belonging to the LysM receptor-like kinase (LysM-RLK) phylogenetic group LYR-IA play a role in AM establishment, but it is unclear which proteins are the plant high-affinity short-chain CO receptors. Here we studied members of the uncharacterized LYR-IB group, and found that they show high affinity for LCO, short- and long-chain CO, and play a complementary role with the LYR-IA receptors for AM establishment. While LYR-IB knock out mutants had a reduced AMF colonization in several species, constitutive/ectopic expression in wheat increased AMF colonization. LYR-IB function is conserved in all tested angiosperms, but in most japonica rice a deletion creates a frameshift in the gene, explaining differences in AM phenotypes between rice and other monocot single LYR-IA mutants. In conclusion, we identified a class of LysM-RLK receptors in angiosperms with unique biochemical properties and a role in both LCO and CO perception for AM establishment. |
| format | Article |
| id | doaj-art-71160841a9664dc5a5777467d3fe5fc7 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-71160841a9664dc5a5777467d3fe5fc72025-08-20T03:37:38ZengNature PortfolioNature Communications2041-17232025-07-0116111410.1038/s41467-025-60717-1Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishmentYi Ding0Zélie Lesterps1Virginie Gasciolli2Anne-Laure Fuchs3Mégane Gaston4Lauréna Medioni5Annélie de-Regibus6Céline Remblière7Céline Vicédo8Sandra Bensmihen9Jean Jacques Bono10Julie Cullimore11Guilhem Reyt12Marion Dalmais13Christine Saffray14Solène Mazeau15Abdelhafid Bendahmane16Richard Sibout17Michiel Vandenbussche18Jacques Rouster19Tongming Wang20Guanghua He21Arnaud Masselin22Sylvain Cottaz23Sébastien Fort24Benoit Lefebvre25Laboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSUniversité Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2)Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2)Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2)Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2)INRAE, UR1268 BIALaboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAELimagrain Field Seeds, Traits and Technologies, Centre de Recherche de ChappesLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSRice Research Institute, Key Laboratory of Application and Safety Control of Genetically Modified Crops, Academy of Agricultural Sciences, Southwest UniversityUniv. Grenoble Alpes, CNRS, CERMAVUniv. Grenoble Alpes, CNRS, CERMAVUniv. Grenoble Alpes, CNRS, CERMAVLaboratory of Plant-Microbe-Environment Interactions (LIPME), Université de Toulouse, INRAE, CNRSAbstract Lipo-chitooligosaccharides (LCO) and short-chain chitooligosaccharides (CO) are produced by arbuscular mycorrhizal fungi (AMF) and activate the plant symbiosis signalling pathway, which is essential for mycorrhiza formation. High-affinity LCO receptors belonging to the LysM receptor-like kinase (LysM-RLK) phylogenetic group LYR-IA play a role in AM establishment, but it is unclear which proteins are the plant high-affinity short-chain CO receptors. Here we studied members of the uncharacterized LYR-IB group, and found that they show high affinity for LCO, short- and long-chain CO, and play a complementary role with the LYR-IA receptors for AM establishment. While LYR-IB knock out mutants had a reduced AMF colonization in several species, constitutive/ectopic expression in wheat increased AMF colonization. LYR-IB function is conserved in all tested angiosperms, but in most japonica rice a deletion creates a frameshift in the gene, explaining differences in AM phenotypes between rice and other monocot single LYR-IA mutants. In conclusion, we identified a class of LysM-RLK receptors in angiosperms with unique biochemical properties and a role in both LCO and CO perception for AM establishment.https://doi.org/10.1038/s41467-025-60717-1 |
| spellingShingle | Yi Ding Zélie Lesterps Virginie Gasciolli Anne-Laure Fuchs Mégane Gaston Lauréna Medioni Annélie de-Regibus Céline Remblière Céline Vicédo Sandra Bensmihen Jean Jacques Bono Julie Cullimore Guilhem Reyt Marion Dalmais Christine Saffray Solène Mazeau Abdelhafid Bendahmane Richard Sibout Michiel Vandenbussche Jacques Rouster Tongming Wang Guanghua He Arnaud Masselin Sylvain Cottaz Sébastien Fort Benoit Lefebvre Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment Nature Communications |
| title | Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment |
| title_full | Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment |
| title_fullStr | Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment |
| title_full_unstemmed | Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment |
| title_short | Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment |
| title_sort | several groups of lysm rlks are involved in symbiotic signal perception and arbuscular mycorrhiza establishment |
| url | https://doi.org/10.1038/s41467-025-60717-1 |
| work_keys_str_mv | AT yiding severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT zelielesterps severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT virginiegasciolli severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT annelaurefuchs severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT meganegaston severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT laurenamedioni severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT anneliederegibus severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT celinerembliere severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT celinevicedo severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT sandrabensmihen severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT jeanjacquesbono severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT juliecullimore severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT guilhemreyt severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT mariondalmais severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT christinesaffray severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT solenemazeau severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT abdelhafidbendahmane severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT richardsibout severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT michielvandenbussche severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT jacquesrouster severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT tongmingwang severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT guanghuahe severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT arnaudmasselin severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT sylvaincottaz severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT sebastienfort severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment AT benoitlefebvre severalgroupsoflysmrlksareinvolvedinsymbioticsignalperceptionandarbuscularmycorrhizaestablishment |