Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land
IntroductionLand cultivation is the cornerstone of national food security. However, with the development of non-grain production on cultivated land, China has to use less cultivated land to feed a larger population of the world. To effectively resolve issues caused by non-grain production on cultiva...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1618073/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849763060387938304 |
|---|---|
| author | Xuqing Li Xiaoxu Ren Han Chen Yukang Xin Tiefeng Zhou Jianli Yan Jun Xu Munazza Ijaz Temoor Ahmed Temoor Ahmed Bin Li Qurban Ali |
| author_facet | Xuqing Li Xiaoxu Ren Han Chen Yukang Xin Tiefeng Zhou Jianli Yan Jun Xu Munazza Ijaz Temoor Ahmed Temoor Ahmed Bin Li Qurban Ali |
| author_sort | Xuqing Li |
| collection | DOAJ |
| description | IntroductionLand cultivation is the cornerstone of national food security. However, with the development of non-grain production on cultivated land, China has to use less cultivated land to feed a larger population of the world. To effectively resolve issues caused by non-grain production on cultivated land, Zhejiang Province has initiated efforts to restore non-grain-converted land back to grain cultivation. Whereas, the discovery and application of plant growth-promoting fungi (PGPF) can offer promising solutions to these challenges.MethodsPGPF was isolated and identified from soil converted from non-grain lands based on bioassays for plant growth promoting traits, and then their impacts on soil properties and microbial community structure were also investigated.ResultsIn this study, 15 fungal isolates from 108 soil samples were considered as potential PGPF due to their ability to solubilize phosphate (11.91 to 31.65 mm), produce both siderophores (17.09 to 24.66 mm) and indole-3-acetic acid (8.79 to 50.23 μg/mL or 36.72 to 96.50 μg/mL). Results of in vivo assays showed that isolates TL-B31f and FY-R41f could cause a great increase in plant height (15.30% and 13.84%), root length (33.62% and 43.31%), seedling fresh weight (78.58% and 89.77%) and dry weight (9.31% and 28.12%) of rice compared to the control. Based on morphological and molecular analyses, isolates TL-B31f and FY-R41f were identified as Aspergillus tubingensis and Talaromyces veerkampii, respectively. Furthermore, after 55 days of inoculation with the two isolates, the soil content of available phosphate was significantly increased by 42.52% and 48.51%, respectively, compared to the control. In addition, high-throughput sequencing analysis showed that compared with the control, the microbial community composition of the two isolates treatments was reconstructed by increasing or decreasing some specific microbes, while soil properties, such as pH, soil organic matter (SOM), total phosphorus (TP), and available phosphate (AP) might play important roles in modulating rice growth by influencing the composition of microbial communities.ConclusionsOverall, our findings highlight the potential of these isolates to be developed into novel biofertilizers for crop growth in non-grain lands. |
| format | Article |
| id | doaj-art-710262d59864494dbfa2fa2464c214fa |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-710262d59864494dbfa2fa2464c214fa2025-08-20T03:05:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-08-011610.3389/fpls.2025.16180731618073Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted landXuqing Li0Xiaoxu Ren1Han Chen2Yukang Xin3Tiefeng Zhou4Jianli Yan5Jun Xu6Munazza Ijaz7Temoor Ahmed8Temoor Ahmed9Bin Li10Qurban Ali11Institute of Vegetable, Hangzhou Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetable, Hangzhou Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetable, Hangzhou Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang Engineering Research Center for Biological Control of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaInstitute of Vegetable, Hangzhou Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetable, Hangzhou Academy of Agricultural Sciences, Hangzhou, ChinaInstitute for Plant Protection and Fertilizer, Agricultural Technology Extension Center of Fuyang District, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang Engineering Research Center for Biological Control of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Life Sciences, Western Caspian University, Baku, AzerbaijanXianghu Laboratory, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang Engineering Research Center for Biological Control of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesIntroductionLand cultivation is the cornerstone of national food security. However, with the development of non-grain production on cultivated land, China has to use less cultivated land to feed a larger population of the world. To effectively resolve issues caused by non-grain production on cultivated land, Zhejiang Province has initiated efforts to restore non-grain-converted land back to grain cultivation. Whereas, the discovery and application of plant growth-promoting fungi (PGPF) can offer promising solutions to these challenges.MethodsPGPF was isolated and identified from soil converted from non-grain lands based on bioassays for plant growth promoting traits, and then their impacts on soil properties and microbial community structure were also investigated.ResultsIn this study, 15 fungal isolates from 108 soil samples were considered as potential PGPF due to their ability to solubilize phosphate (11.91 to 31.65 mm), produce both siderophores (17.09 to 24.66 mm) and indole-3-acetic acid (8.79 to 50.23 μg/mL or 36.72 to 96.50 μg/mL). Results of in vivo assays showed that isolates TL-B31f and FY-R41f could cause a great increase in plant height (15.30% and 13.84%), root length (33.62% and 43.31%), seedling fresh weight (78.58% and 89.77%) and dry weight (9.31% and 28.12%) of rice compared to the control. Based on morphological and molecular analyses, isolates TL-B31f and FY-R41f were identified as Aspergillus tubingensis and Talaromyces veerkampii, respectively. Furthermore, after 55 days of inoculation with the two isolates, the soil content of available phosphate was significantly increased by 42.52% and 48.51%, respectively, compared to the control. In addition, high-throughput sequencing analysis showed that compared with the control, the microbial community composition of the two isolates treatments was reconstructed by increasing or decreasing some specific microbes, while soil properties, such as pH, soil organic matter (SOM), total phosphorus (TP), and available phosphate (AP) might play important roles in modulating rice growth by influencing the composition of microbial communities.ConclusionsOverall, our findings highlight the potential of these isolates to be developed into novel biofertilizers for crop growth in non-grain lands.https://www.frontiersin.org/articles/10.3389/fpls.2025.1618073/fullnon-grain converted landsplant growth promoting fungiidentificationsoil propertiesmicrobial community |
| spellingShingle | Xuqing Li Xiaoxu Ren Han Chen Yukang Xin Tiefeng Zhou Jianli Yan Jun Xu Munazza Ijaz Temoor Ahmed Temoor Ahmed Bin Li Qurban Ali Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land Frontiers in Plant Science non-grain converted lands plant growth promoting fungi identification soil properties microbial community |
| title | Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land |
| title_full | Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land |
| title_fullStr | Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land |
| title_full_unstemmed | Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land |
| title_short | Isolation of multiple plant growth-promoting fungi and their effect on rice growth improvement on non-grain converted land |
| title_sort | isolation of multiple plant growth promoting fungi and their effect on rice growth improvement on non grain converted land |
| topic | non-grain converted lands plant growth promoting fungi identification soil properties microbial community |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1618073/full |
| work_keys_str_mv | AT xuqingli isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT xiaoxuren isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT hanchen isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT yukangxin isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT tiefengzhou isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT jianliyan isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT junxu isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT munazzaijaz isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT temoorahmed isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT temoorahmed isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT binli isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland AT qurbanali isolationofmultipleplantgrowthpromotingfungiandtheireffectonricegrowthimprovementonnongrainconvertedland |