Kinetics of Enantiomerically Enriched Synthesis of Solketal Esters Using Native and SBA-15 supported P. Fluorescens Lipase
The studies showed that alkaline lipase from Pseudomonas fluorescens enables an irreversible transesterification of vinyl esters to give enantiomeric excess (eeR) of about 80% using vinyl butyrate as acyl donor and diisopropyl ether as a solvent, at partially optimized conditions. For the native lip...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences Committee of Chemical and Process Engineering
2017-06-01
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| Series: | Chemical and Process Engineering |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/cpe.2017.38.issue-2/cpe-2017-0016/cpe-2017-0016.xml?format=INT |
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| Summary: | The studies showed that alkaline lipase from Pseudomonas fluorescens enables an irreversible transesterification of vinyl esters to give enantiomeric excess (eeR) of about 80% using vinyl butyrate as acyl donor and diisopropyl ether as a solvent, at partially optimized conditions. For the native lipase the process was adequately described by a five-parameter Ping-Pong Bi Bi model for both enantiomers plus expression accounting for the formation of enzyme-acyl donor complex, but for the same lipase supported on mesoporous materials of SBA-15-Oc type, R-product inhibition also had to be taken into account. The use of hydrophobic support increased by more than two-fold the rate of the S-solketal conversion but even more that of R-solketal. Thus the immobilization of lipase had very positive effect on the process kinetics but decreased its enantioselectivity. |
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| ISSN: | 2300-1925 |