Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs
Staphylococcus aureus (S. aureus) is bacterium that threatens public health because it causes infections and food intoxication. For this reason, within the scope of this study, it was aimed to determine the presence of S. aureus, antibiotic resistance profiles, and biofilm formation in ground beef a...
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Sakarya University
2024-06-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/3474988 |
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| author | Tuğba Sarı Ceren Baskan |
| author_facet | Tuğba Sarı Ceren Baskan |
| author_sort | Tuğba Sarı |
| collection | DOAJ |
| description | Staphylococcus aureus (S. aureus) is bacterium that threatens public health because it causes infections and food intoxication. For this reason, within the scope of this study, it was aimed to determine the presence of S. aureus, antibiotic resistance profiles, and biofilm formation in ground beef and meatball samples consumed in Amasya. In the study, 60 meat samples purchased from Amasya were used as material. First, conventional culture technique and PCR testing were used for the isolation of S. aureus. Secondly, antibiotic resistance profiles of the S. aureus were analyzed by disc diffusion. Finally, biofilm production of the S. aureus was analyzed phenotypically by the microtiter plate method and genotypically by PCR. Through the study, we identified 58 S. aureus isolates that were confirmed phenotypically and genotypically. Disc diffusion results showed that all S. aureus were sensitive to imipenem and piperacillin-tazobactam, but resistant to methicillin 43.10% (25/58), erythromycin 41.37 % (24/58), penicillin 58.62% (34/58), gentamicin 10.34% (6/58), chloramphenicol 17.24% (10/58), tobramycin 6.89% (4/58), and levofloxacin 1.72% (1/58). Biofilm production was determined as 58.62% (34/58) in the microtiter plate. In the PCR analysis, the icaA or icaD gene of a total of 4 (6.89%) different isolates was evaluated as positive. As a result, the presence of antibiotic-resistant S. aureus in ground beef and meatballs and the production of biofilm by the bacteria pose a potential risk. Therefore, it is important for human health to take the necessary precautions to reduce the risk of S. aureus contamination during the processing, and transportation. |
| format | Article |
| id | doaj-art-70fa14f1c1254a7285faefcd6e8707c8 |
| institution | Kabale University |
| issn | 2147-835X |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-70fa14f1c1254a7285faefcd6e8707c82024-12-23T08:17:32ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2024-06-0128350551610.16984/saufenbilder.137602828Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and MeatballsTuğba Sarı0https://orcid.org/0009-0006-9166-4647Ceren Baskan1https://orcid.org/0000-0001-7849-4459AMASYA UNIVERSITYAMASYA ÜNİVERSİTESİStaphylococcus aureus (S. aureus) is bacterium that threatens public health because it causes infections and food intoxication. For this reason, within the scope of this study, it was aimed to determine the presence of S. aureus, antibiotic resistance profiles, and biofilm formation in ground beef and meatball samples consumed in Amasya. In the study, 60 meat samples purchased from Amasya were used as material. First, conventional culture technique and PCR testing were used for the isolation of S. aureus. Secondly, antibiotic resistance profiles of the S. aureus were analyzed by disc diffusion. Finally, biofilm production of the S. aureus was analyzed phenotypically by the microtiter plate method and genotypically by PCR. Through the study, we identified 58 S. aureus isolates that were confirmed phenotypically and genotypically. Disc diffusion results showed that all S. aureus were sensitive to imipenem and piperacillin-tazobactam, but resistant to methicillin 43.10% (25/58), erythromycin 41.37 % (24/58), penicillin 58.62% (34/58), gentamicin 10.34% (6/58), chloramphenicol 17.24% (10/58), tobramycin 6.89% (4/58), and levofloxacin 1.72% (1/58). Biofilm production was determined as 58.62% (34/58) in the microtiter plate. In the PCR analysis, the icaA or icaD gene of a total of 4 (6.89%) different isolates was evaluated as positive. As a result, the presence of antibiotic-resistant S. aureus in ground beef and meatballs and the production of biofilm by the bacteria pose a potential risk. Therefore, it is important for human health to take the necessary precautions to reduce the risk of S. aureus contamination during the processing, and transportation.https://dergipark.org.tr/tr/download/article-file/3474988antibiotic resistancebiofilmground beefmeatballss. aureus |
| spellingShingle | Tuğba Sarı Ceren Baskan Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi antibiotic resistance biofilm ground beef meatballs s. aureus |
| title | Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs |
| title_full | Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs |
| title_fullStr | Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs |
| title_full_unstemmed | Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs |
| title_short | Investigation of Biofilm Production and Determination of Antibiotic Resistance Profile of Staphylococcus aureus Isolated from Ground Beef and Meatballs |
| title_sort | investigation of biofilm production and determination of antibiotic resistance profile of staphylococcus aureus isolated from ground beef and meatballs |
| topic | antibiotic resistance biofilm ground beef meatballs s. aureus |
| url | https://dergipark.org.tr/tr/download/article-file/3474988 |
| work_keys_str_mv | AT tugbasarı investigationofbiofilmproductionanddeterminationofantibioticresistanceprofileofstaphylococcusaureusisolatedfromgroundbeefandmeatballs AT cerenbaskan investigationofbiofilmproductionanddeterminationofantibioticresistanceprofileofstaphylococcusaureusisolatedfromgroundbeefandmeatballs |