Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity
Abstract In this study, we conducted a comprehensive investigation into the Enterobacter cloacae complex (ECC), a group of notorious pathogens responsible for various hospital-acquired infections. We aimed to gain critical insights into antimicrobial resistance profiles and genomic diversity among 1...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-81595-5 |
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| author | Thunchanok Yaikhan Kamonnut Singkhamanan Pawarisa Luenglusontigit Arnon Chukamnerd Natakorn Nokchan Nutwadee Chintakovid Sarunyou Chusri Rattanaruji Pomwised Monwadee Wonglapsuwan Kittinun Leetanaporn Surasak Sangkhathat Komwit Surachat |
| author_facet | Thunchanok Yaikhan Kamonnut Singkhamanan Pawarisa Luenglusontigit Arnon Chukamnerd Natakorn Nokchan Nutwadee Chintakovid Sarunyou Chusri Rattanaruji Pomwised Monwadee Wonglapsuwan Kittinun Leetanaporn Surasak Sangkhathat Komwit Surachat |
| author_sort | Thunchanok Yaikhan |
| collection | DOAJ |
| description | Abstract In this study, we conducted a comprehensive investigation into the Enterobacter cloacae complex (ECC), a group of notorious pathogens responsible for various hospital-acquired infections. We aimed to gain critical insights into antimicrobial resistance profiles and genomic diversity among 17 ECC isolates, which were previously collected as part of a short-term surveillance effort for 6 months in 2019. We identified two novel sequence types (ST-1936 in E. bugandensis PSU30 and ST-1937 in E. roggenkampii PSU45) among the 14 distinct STs identified in our ECC isolates. Furthermore, our expanded investigation revealed 296 novel STs within the NCBI Reference Sequence database. We identified six isolates carrying the mcr-9 gene, highlighting a significant concern in antimicrobial resistance (AMR). These genes confer a reduced susceptibility to colistin, a critical last-resort drug for the treatment of multidrug-resistant (MDR) infection. In addition to the AMR complexity, we found that three isolates carried the bla NDM gene on IncN2 plasmids, further emphasizing the urgency of monitoring and managing ECC-related infections. Our study provided evidence of intra-hospital transmission involving E. asburiae isolates PSU37, PSU39, and PSU40, all collected from the nasopharynx of three individuals in the intensive care unit (ICU) of the same hospital. These findings highlight the need for stringent infection control measures to prevent similar outbreaks and emphasize the importance of effective surveillance and management strategies to address ECC-related challenges. |
| format | Article |
| id | doaj-art-70f4bec5c4df42fdb55355171b43d0ca |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-70f4bec5c4df42fdb55355171b43d0ca2025-02-09T12:32:49ZengNature PortfolioScientific Reports2045-23222025-02-0115111310.1038/s41598-024-81595-5Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversityThunchanok Yaikhan0Kamonnut Singkhamanan1Pawarisa Luenglusontigit2Arnon Chukamnerd3Natakorn Nokchan4Nutwadee Chintakovid5Sarunyou Chusri6Rattanaruji Pomwised7Monwadee Wonglapsuwan8Kittinun Leetanaporn9Surasak Sangkhathat10Komwit Surachat11Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityDivision of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla UniversityDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityDivision of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla UniversityDivision of Biological Science, Faculty of Science, Prince of Songkla UniversityDivision of Biological Science, Faculty of Science, Prince of Songkla UniversityDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityTranslational Medicine Research Center, Faculty of Medicine, Prince of Songkla UniversityDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla UniversityAbstract In this study, we conducted a comprehensive investigation into the Enterobacter cloacae complex (ECC), a group of notorious pathogens responsible for various hospital-acquired infections. We aimed to gain critical insights into antimicrobial resistance profiles and genomic diversity among 17 ECC isolates, which were previously collected as part of a short-term surveillance effort for 6 months in 2019. We identified two novel sequence types (ST-1936 in E. bugandensis PSU30 and ST-1937 in E. roggenkampii PSU45) among the 14 distinct STs identified in our ECC isolates. Furthermore, our expanded investigation revealed 296 novel STs within the NCBI Reference Sequence database. We identified six isolates carrying the mcr-9 gene, highlighting a significant concern in antimicrobial resistance (AMR). These genes confer a reduced susceptibility to colistin, a critical last-resort drug for the treatment of multidrug-resistant (MDR) infection. In addition to the AMR complexity, we found that three isolates carried the bla NDM gene on IncN2 plasmids, further emphasizing the urgency of monitoring and managing ECC-related infections. Our study provided evidence of intra-hospital transmission involving E. asburiae isolates PSU37, PSU39, and PSU40, all collected from the nasopharynx of three individuals in the intensive care unit (ICU) of the same hospital. These findings highlight the need for stringent infection control measures to prevent similar outbreaks and emphasize the importance of effective surveillance and management strategies to address ECC-related challenges.https://doi.org/10.1038/s41598-024-81595-5Enterobacter cloacae complexNovel STWhole genome sequencingComprehensive genomic profiling |
| spellingShingle | Thunchanok Yaikhan Kamonnut Singkhamanan Pawarisa Luenglusontigit Arnon Chukamnerd Natakorn Nokchan Nutwadee Chintakovid Sarunyou Chusri Rattanaruji Pomwised Monwadee Wonglapsuwan Kittinun Leetanaporn Surasak Sangkhathat Komwit Surachat Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity Scientific Reports Enterobacter cloacae complex Novel ST Whole genome sequencing Comprehensive genomic profiling |
| title | Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity |
| title_full | Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity |
| title_fullStr | Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity |
| title_full_unstemmed | Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity |
| title_short | Genomic analysis of Enterobacter cloacae complex from Southern Thailand reveals insights into multidrug resistance genotypes and genetic diversity |
| title_sort | genomic analysis of enterobacter cloacae complex from southern thailand reveals insights into multidrug resistance genotypes and genetic diversity |
| topic | Enterobacter cloacae complex Novel ST Whole genome sequencing Comprehensive genomic profiling |
| url | https://doi.org/10.1038/s41598-024-81595-5 |
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