Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development
Abstract This study elucidated the role of DHA-modulated genes in the development and growth of Atlantic bluefin tuna (Thunnus thynnus) larvae ingesting increasing levels of DHA in their rotifer prey. The effect of feeding low, medium, and high rotifer (Brachionus rotundiformis) DHA levels (2.0, 3.6...
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Nature Portfolio
2024-10-01
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| Online Access: | https://doi.org/10.1038/s41598-024-74152-7 |
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| author | William Koven Eran Yanowski Luke Gardner Oriya Nixon Barbara Block |
| author_facet | William Koven Eran Yanowski Luke Gardner Oriya Nixon Barbara Block |
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| description | Abstract This study elucidated the role of DHA-modulated genes in the development and growth of Atlantic bluefin tuna (Thunnus thynnus) larvae ingesting increasing levels of DHA in their rotifer prey. The effect of feeding low, medium, and high rotifer (Brachionus rotundiformis) DHA levels (2.0, 3.6 and 10.9 mg DHA g−1 DW, respectively) was tested on 2–15 days post hatching (dph) bluefin tuna larvae. Larval DHA content markedly (P < 0.05) increased in a DHA dose-dependent manner (1.5, 3.9, 6.1 mg DHA g−1 DW larva, respectively), that was positively correlated with larval prey consumption and growth (P < 0.05). Gene ontology enrichment analyses of differentially expressed genes (DEGs) demonstrated dietary DHA significantly (P < 0.05) affected different genes and biological processes at different developmental ages. The number of DHA up-regulated DEGs was highest in 10 dph larvae (491), compared to 5 (12) and 15 dph fish (34), and were mainly involved in neural and synaptic development in the brain and spinal cord. In contrast, DHA in older 15 dph larvae elicited fewer DEGs but played critical roles over a wider range of developing organs. The emerging picture underscores the importance of DHA-modulated gene expression as a driving force in bluefin tuna larval development and growth. |
| format | Article |
| id | doaj-art-70e50b2c03fc4e52a3cc870761554835 |
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| publishDate | 2024-10-01 |
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| spelling | doaj-art-70e50b2c03fc4e52a3cc8707615548352025-08-20T02:31:03ZengNature PortfolioScientific Reports2045-23222024-10-0114111410.1038/s41598-024-74152-7Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae developmentWilliam Koven0Eran Yanowski1Luke Gardner2Oriya Nixon3Barbara Block4Israel Oceanographic and Limnological Research, The National Center for Mariculture (NCM)Israel Oceanographic and Limnological Research, The National Center for Mariculture (NCM)Hopkins Marine Station of Stanford UniversityIsrael Oceanographic and Limnological Research, The National Center for Mariculture (NCM)Hopkins Marine Station of Stanford UniversityAbstract This study elucidated the role of DHA-modulated genes in the development and growth of Atlantic bluefin tuna (Thunnus thynnus) larvae ingesting increasing levels of DHA in their rotifer prey. The effect of feeding low, medium, and high rotifer (Brachionus rotundiformis) DHA levels (2.0, 3.6 and 10.9 mg DHA g−1 DW, respectively) was tested on 2–15 days post hatching (dph) bluefin tuna larvae. Larval DHA content markedly (P < 0.05) increased in a DHA dose-dependent manner (1.5, 3.9, 6.1 mg DHA g−1 DW larva, respectively), that was positively correlated with larval prey consumption and growth (P < 0.05). Gene ontology enrichment analyses of differentially expressed genes (DEGs) demonstrated dietary DHA significantly (P < 0.05) affected different genes and biological processes at different developmental ages. The number of DHA up-regulated DEGs was highest in 10 dph larvae (491), compared to 5 (12) and 15 dph fish (34), and were mainly involved in neural and synaptic development in the brain and spinal cord. In contrast, DHA in older 15 dph larvae elicited fewer DEGs but played critical roles over a wider range of developing organs. The emerging picture underscores the importance of DHA-modulated gene expression as a driving force in bluefin tuna larval development and growth.https://doi.org/10.1038/s41598-024-74152-7Gene regulationBluefin tunaLarvaeDocosahexaenoic acidNeuronsSynaptic function |
| spellingShingle | William Koven Eran Yanowski Luke Gardner Oriya Nixon Barbara Block Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development Scientific Reports Gene regulation Bluefin tuna Larvae Docosahexaenoic acid Neurons Synaptic function |
| title | Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development |
| title_full | Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development |
| title_fullStr | Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development |
| title_full_unstemmed | Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development |
| title_short | Docosahexaenoic acid (DHA) is a driving force regulating gene expression in bluefin tuna (Thunnus thynnus) larvae development |
| title_sort | docosahexaenoic acid dha is a driving force regulating gene expression in bluefin tuna thunnus thynnus larvae development |
| topic | Gene regulation Bluefin tuna Larvae Docosahexaenoic acid Neurons Synaptic function |
| url | https://doi.org/10.1038/s41598-024-74152-7 |
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