Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol
Objective: Type-2 diabetes (T2D) is a multifactorial disease that occurs as a result of impaired insulin secretion and increased glucose. Researchers emphasize that oxidative stress prevention and improvement strategies are important in the general treatment of T2D. It is reported that delta-9-tetra...
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Istanbul University Press
2021-12-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C4AB0896A1E4466DBBE21CDC62997F5C |
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| author | Ebrar Tatar Beti Pesen Zeynep Mine Coşkun Yazıcı Sema Bolkent |
| author_facet | Ebrar Tatar Beti Pesen Zeynep Mine Coşkun Yazıcı Sema Bolkent |
| author_sort | Ebrar Tatar |
| collection | DOAJ |
| description | Objective: Type-2 diabetes (T2D) is a multifactorial disease that occurs as a result of impaired insulin secretion and increased glucose. Researchers emphasize that oxidative stress prevention and improvement strategies are important in the general treatment of T2D. It is reported that delta-9-tetrahydrocannabinol (THC), a component of the cannabis plant, has positive effects against oxidative stress and inflammation. Therefore, this study explored the inhibitory effects of THC on oxidative damage of testis in type-2 diabetic rats. Materials and Methods: Adult Spraque-Dawley rats were separated into 4 groups. In the control group, physiological saline was given intraperitoneally. In the T2D model group (T2DM), streptozotocin (STZ) + nicotinamide (NAD) was administered intraperitoneally. Three mg/kg/day THC was given to the THC and T2DM+THC groups for 7 days, intraperitoneally. Glutathione (GSH), malondialdehyde (MDA), and protein carbonyl levels (PCO), and superoxide dismutase (SOD) activity were measured spectrophotometrically in testicular tissue. Total oxidant and antioxidant status were determined by ELISA. Results: Testicular GSH level and SOD activity were significantly decreased in T2D, while MDA and PCO levels increased. In contrast to this effect, the THC treatment increased GSH levels and SOD activity in diabetics, but decreased MDA and PCO levels. Conclusion: According to the study results, THC may have an oxidative stress inhibitory effect on diabetic rat testis. |
| format | Article |
| id | doaj-art-70e455e239bf4961a5d2a8d885e6c21c |
| institution | OA Journals |
| issn | 2618-6144 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-70e455e239bf4961a5d2a8d885e6c21c2025-08-20T02:10:56ZengIstanbul University PressEuropean Journal of Biology2618-61442021-12-01802919610.26650/EurJBiol.2021.1001540123456Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-TetrahydrocannabinolEbrar Tatar0https://orcid.org/0000-0003-2273-7487Beti Pesen1https://orcid.org/0000-0003-1449-9749Zeynep Mine Coşkun Yazıcı2https://orcid.org/0000-0003-4791-6537Sema Bolkent3https://orcid.org/0000-0001-8463-5561Demiroğlu Bilim Üniversitesi, Istanbul, TurkiyeDemiroğlu Bilim Üniversitesi, Istanbul, TurkiyeDemiroğlu Bilim Üniversitesi, Istanbul, Turkiyeİstanbul Üniversitesi-Cerrahpaşa, Istanbul, TurkiyeObjective: Type-2 diabetes (T2D) is a multifactorial disease that occurs as a result of impaired insulin secretion and increased glucose. Researchers emphasize that oxidative stress prevention and improvement strategies are important in the general treatment of T2D. It is reported that delta-9-tetrahydrocannabinol (THC), a component of the cannabis plant, has positive effects against oxidative stress and inflammation. Therefore, this study explored the inhibitory effects of THC on oxidative damage of testis in type-2 diabetic rats. Materials and Methods: Adult Spraque-Dawley rats were separated into 4 groups. In the control group, physiological saline was given intraperitoneally. In the T2D model group (T2DM), streptozotocin (STZ) + nicotinamide (NAD) was administered intraperitoneally. Three mg/kg/day THC was given to the THC and T2DM+THC groups for 7 days, intraperitoneally. Glutathione (GSH), malondialdehyde (MDA), and protein carbonyl levels (PCO), and superoxide dismutase (SOD) activity were measured spectrophotometrically in testicular tissue. Total oxidant and antioxidant status were determined by ELISA. Results: Testicular GSH level and SOD activity were significantly decreased in T2D, while MDA and PCO levels increased. In contrast to this effect, the THC treatment increased GSH levels and SOD activity in diabetics, but decreased MDA and PCO levels. Conclusion: According to the study results, THC may have an oxidative stress inhibitory effect on diabetic rat testis.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C4AB0896A1E4466DBBE21CDC62997F5Cdiabetestetrahydrocannabinoltestisoxidative stress |
| spellingShingle | Ebrar Tatar Beti Pesen Zeynep Mine Coşkun Yazıcı Sema Bolkent Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol European Journal of Biology diabetes tetrahydrocannabinol testis oxidative stress |
| title | Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol |
| title_full | Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol |
| title_fullStr | Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol |
| title_full_unstemmed | Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol |
| title_short | Oxidative Stress Status in Testis of Type-2 Diabetic Rats Treated with Delta-9-Tetrahydrocannabinol |
| title_sort | oxidative stress status in testis of type 2 diabetic rats treated with delta 9 tetrahydrocannabinol |
| topic | diabetes tetrahydrocannabinol testis oxidative stress |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C4AB0896A1E4466DBBE21CDC62997F5C |
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