Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i>
The typical clinical signs of <i>Nocardia seriolae</i> infection include white nodules, ranging from 0.1 to 10 mm in diameter, distributed across various internal organs. However, the structural composition of nodules of different sizes remains unexplored. In this study, natural cases of...
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MDPI AG
2024-12-01
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| author | Chengwei Li Wenhui Wang Fan Wu Hao Pu Lei Liao |
| author_facet | Chengwei Li Wenhui Wang Fan Wu Hao Pu Lei Liao |
| author_sort | Chengwei Li |
| collection | DOAJ |
| description | The typical clinical signs of <i>Nocardia seriolae</i> infection include white nodules, ranging from 0.1 to 10 mm in diameter, distributed across various internal organs. However, the structural composition of nodules of different sizes remains unexplored. In this study, natural cases of largemouth bass (<i>Micropterus salmoides</i>) were collected, and pathogenic bacteria were isolated and confirmed through a re-infection experiment. The isolated bacteria were identified as <i>N. seriolae</i> through <i>16S rRNA</i> and <i>gyrB</i> gene sequencing. Healthy largemouth bass were infected with the isolate using an immersion infection and observed continuously over 56 days. Samples were successfully obtained from the incubation, prodromal, symptomatic, and convalescent phases, allowing for gross, histological, and ultrastructural observations of nodular lesion progression. Results demonstrated two types of nodular lesions: necrotic foci and granulomas. Macroscopically visible nodules larger than 1 mm, observed primarily in the liver, spleen, kidney, and muscle tissues of moribund fish, exhibited coagulative necrosis and were identified as the principal cause of mortality. Conversely, granulomas, with diameters less than 1 mm, were consistently present in the spleen, kidney, and liver during the symptomatic and convalescent phases. |
| format | Article |
| id | doaj-art-70d9abb4e7804edda92096784638e828 |
| institution | DOAJ |
| issn | 2410-3888 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Fishes |
| spelling | doaj-art-70d9abb4e7804edda92096784638e8282025-08-20T02:55:37ZengMDPI AGFishes2410-38882024-12-0191251510.3390/fishes9120515Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i>Chengwei Li0Wenhui Wang1Fan Wu2Hao Pu3Lei Liao4College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Husbandry and Veterinary, Chengdu Agricultural College, Chengdu 611130, ChinaCollege of Animal Husbandry and Veterinary, Chengdu Agricultural College, Chengdu 611130, ChinaCollege of Animal Husbandry and Veterinary, Chengdu Agricultural College, Chengdu 611130, ChinaThe typical clinical signs of <i>Nocardia seriolae</i> infection include white nodules, ranging from 0.1 to 10 mm in diameter, distributed across various internal organs. However, the structural composition of nodules of different sizes remains unexplored. In this study, natural cases of largemouth bass (<i>Micropterus salmoides</i>) were collected, and pathogenic bacteria were isolated and confirmed through a re-infection experiment. The isolated bacteria were identified as <i>N. seriolae</i> through <i>16S rRNA</i> and <i>gyrB</i> gene sequencing. Healthy largemouth bass were infected with the isolate using an immersion infection and observed continuously over 56 days. Samples were successfully obtained from the incubation, prodromal, symptomatic, and convalescent phases, allowing for gross, histological, and ultrastructural observations of nodular lesion progression. Results demonstrated two types of nodular lesions: necrotic foci and granulomas. Macroscopically visible nodules larger than 1 mm, observed primarily in the liver, spleen, kidney, and muscle tissues of moribund fish, exhibited coagulative necrosis and were identified as the principal cause of mortality. Conversely, granulomas, with diameters less than 1 mm, were consistently present in the spleen, kidney, and liver during the symptomatic and convalescent phases.https://www.mdpi.com/2410-3888/9/12/515<i>Nocardia seriolae</i><i>Micropterus salmoides</i>necrotic focigranulomas |
| spellingShingle | Chengwei Li Wenhui Wang Fan Wu Hao Pu Lei Liao Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i> Fishes <i>Nocardia seriolae</i> <i>Micropterus salmoides</i> necrotic foci granulomas |
| title | Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i> |
| title_full | Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i> |
| title_fullStr | Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i> |
| title_full_unstemmed | Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i> |
| title_short | Pathological Progress of Two Types of Nodules in <i>Micropterus salmoides</i> Infected with <i>Nocardia seriolae</i> |
| title_sort | pathological progress of two types of nodules in i micropterus salmoides i infected with i nocardia seriolae i |
| topic | <i>Nocardia seriolae</i> <i>Micropterus salmoides</i> necrotic foci granulomas |
| url | https://www.mdpi.com/2410-3888/9/12/515 |
| work_keys_str_mv | AT chengweili pathologicalprogressoftwotypesofnodulesinimicropterussalmoidesiinfectedwithinocardiaseriolaei AT wenhuiwang pathologicalprogressoftwotypesofnodulesinimicropterussalmoidesiinfectedwithinocardiaseriolaei AT fanwu pathologicalprogressoftwotypesofnodulesinimicropterussalmoidesiinfectedwithinocardiaseriolaei AT haopu pathologicalprogressoftwotypesofnodulesinimicropterussalmoidesiinfectedwithinocardiaseriolaei AT leiliao pathologicalprogressoftwotypesofnodulesinimicropterussalmoidesiinfectedwithinocardiaseriolaei |