Mimicking immune complexes for efficient antibody responses
Efficient antibody responses are crucial for combating infectious diseases and vaccination remains a cornerstone of this effort. This study introduces a novel approach for enhancing immune responses in wild-type mice by utilizing pre-formed immune complexes, using the receptor-binding domain (RBD) o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1570487/full |
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| author | Jonathan Schönfelder Omar El Ayoubi Oles Havryliuk Rüdiger Groß Alina Seidel Tamam Bakchoul Jan Münch Hassan Jumaa Corinna S. Setz |
| author_facet | Jonathan Schönfelder Omar El Ayoubi Oles Havryliuk Rüdiger Groß Alina Seidel Tamam Bakchoul Jan Münch Hassan Jumaa Corinna S. Setz |
| author_sort | Jonathan Schönfelder |
| collection | DOAJ |
| description | Efficient antibody responses are crucial for combating infectious diseases and vaccination remains a cornerstone of this effort. This study introduces a novel approach for enhancing immune responses in wild-type mice by utilizing pre-formed immune complexes, using the receptor-binding domain (RBD) of SARS-CoV-2 as a model antigen to illustrate the broader potential of the concept. Specifically, we found that pre-treating the antigen with bis-maleimide, a chemical linker that facilitates protein cross-linking, significantly enhances antibody production. Moreover, in vitro cross-linking of antigen to unrelated IgG using bis-maleimide generated immune complexes that markedly enhanced antigen-specific antibody responses, likely by mimicking natural memory-like mechanisms, suggesting that bis-maleimide pre-treated antigens may similarly engage IgG in vivo. In contrast, antigen crosslinking with IgA or IgM did not yield comparable effects, highlighting the unique capacity of IgG to boost immunogenicity. By leveraging the principles of immune memory, this study demonstrates the potential of pre-formed immune complexes to significantly enhance vaccine efficacy using an antigen-independent strategy broadly applicable to diverse pathogens. |
| format | Article |
| id | doaj-art-70d76052a0854f84bffdbd2377627f85 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-70d76052a0854f84bffdbd2377627f852025-08-20T02:20:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15704871570487Mimicking immune complexes for efficient antibody responsesJonathan Schönfelder0Omar El Ayoubi1Oles Havryliuk2Rüdiger Groß3Alina Seidel4Tamam Bakchoul5Jan Münch6Hassan Jumaa7Corinna S. Setz8Institute of Immunology, Ulm University Medical Center, Ulm, GermanyInstitute of Immunology, Ulm University Medical Center, Ulm, GermanyInstitute of Immunology, Ulm University Medical Center, Ulm, GermanyInstitute of Molecular Virology, Ulm University Medical Center, Ulm, GermanyInstitute of Molecular Virology, Ulm University Medical Center, Ulm, GermanyCentre for Clinical Transfusion Medicine, University Hospital of Tübingen, Tübingen, GermanyInstitute of Molecular Virology, Ulm University Medical Center, Ulm, GermanyInstitute of Immunology, Ulm University Medical Center, Ulm, GermanyInstitute of Immunology, Ulm University Medical Center, Ulm, GermanyEfficient antibody responses are crucial for combating infectious diseases and vaccination remains a cornerstone of this effort. This study introduces a novel approach for enhancing immune responses in wild-type mice by utilizing pre-formed immune complexes, using the receptor-binding domain (RBD) of SARS-CoV-2 as a model antigen to illustrate the broader potential of the concept. Specifically, we found that pre-treating the antigen with bis-maleimide, a chemical linker that facilitates protein cross-linking, significantly enhances antibody production. Moreover, in vitro cross-linking of antigen to unrelated IgG using bis-maleimide generated immune complexes that markedly enhanced antigen-specific antibody responses, likely by mimicking natural memory-like mechanisms, suggesting that bis-maleimide pre-treated antigens may similarly engage IgG in vivo. In contrast, antigen crosslinking with IgA or IgM did not yield comparable effects, highlighting the unique capacity of IgG to boost immunogenicity. By leveraging the principles of immune memory, this study demonstrates the potential of pre-formed immune complexes to significantly enhance vaccine efficacy using an antigen-independent strategy broadly applicable to diverse pathogens.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1570487/fullIgGB cellsantibody responsesadjuvantmemory |
| spellingShingle | Jonathan Schönfelder Omar El Ayoubi Oles Havryliuk Rüdiger Groß Alina Seidel Tamam Bakchoul Jan Münch Hassan Jumaa Corinna S. Setz Mimicking immune complexes for efficient antibody responses Frontiers in Immunology IgG B cells antibody responses adjuvant memory |
| title | Mimicking immune complexes for efficient antibody responses |
| title_full | Mimicking immune complexes for efficient antibody responses |
| title_fullStr | Mimicking immune complexes for efficient antibody responses |
| title_full_unstemmed | Mimicking immune complexes for efficient antibody responses |
| title_short | Mimicking immune complexes for efficient antibody responses |
| title_sort | mimicking immune complexes for efficient antibody responses |
| topic | IgG B cells antibody responses adjuvant memory |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1570487/full |
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