Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration
Background. Intracerebral hemorrhage (ICH, IH) is a stroke syndrome characterized by high mortality and disability rates. However, the apoptotic mechanisms underlying ICH remain incompletely understood. We employed bioinformatics methods to identify key apoptotic genes associated with ICH, thereby o...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/6189986 |
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| author | Pandi Chen Gengfan Ye Jia Li Kuan Feng Guangyao Zhu Maosong Chen Wei Chen |
| author_facet | Pandi Chen Gengfan Ye Jia Li Kuan Feng Guangyao Zhu Maosong Chen Wei Chen |
| author_sort | Pandi Chen |
| collection | DOAJ |
| description | Background. Intracerebral hemorrhage (ICH, IH) is a stroke syndrome characterized by high mortality and disability rates. However, the apoptotic mechanisms underlying ICH remain incompletely understood. We employed bioinformatics methods to identify key apoptotic genes associated with ICH, thereby offering potential therapeutic targets and contributing to a deeper understanding of the pathogenic mechanisms of apoptosis in ICH. Materials and Methods. We retrieved microarray data of ICH patients from the Gene Expression Omnibus database and intersected it with apoptotic genes, filtering out apoptosis-related differentially expressed genes (ARDEGs). Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted, and a logic regression-based diagnostic model for ICH was constructed. Key genes were identified through least absolute shrinkage and selection operator regression after model validation. Subsequently, mRNA-transcription factor (mRNA-TF), mRNA–microRNA (mRNA–miRNA), mRNA-RNA-binding protein (mRNA-RBP), and mRNA-drug regulatory networks were established, followed by immune infiltration analysis. Results. We identified 29 ARDEGs related to ICH, primarily participating in biological processes such as the extrinsic apoptotic signaling pathway and neuroinflammatory response. The ICH model confirmed PPP1R15A, IL1R1, and TYROBP as key genes with diagnostic and therapeutic value. Thirteen immune cell types showed a correlation with ICH; notably, activated dendritic cells and CD56dim natural killer cells exhibited significant correlations, and key genes were positively correlated with all 13 immune cell types. The regulatory networks (mRNA-TF, mRNA–miRNA, mRNA-RBP, and mRNA-drug) provided insights into the apoptotic gene regulation mechanisms and potential drugs in ICH. Conclusion. Through bioinformatics analysis, we identified PPP1R15A, IL1R1, and TYROBP as crucial genes in the occurrence and progression of ICH, with the potential involvement of Activated dendritic cells and CD56dim natural killer cells in these processes. |
| format | Article |
| id | doaj-art-70cd9729bc7345498d98233bfdeeba3c |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-70cd9729bc7345498d98233bfdeeba3c2025-01-02T22:41:03ZengWileyJournal of Immunology Research2314-71562024-01-01202410.1155/2024/6189986Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune InfiltrationPandi Chen0Gengfan Ye1Jia Li2Kuan Feng3Guangyao Zhu4Maosong Chen5Wei Chen6Department of NeurosurgeryDepartment of NeurosurgeryDepartment of NeurosurgeryDepartment of NeurosurgeryDepartment of NeurosurgeryDepartment of NeurosurgeryDepartment of NeurosurgeryBackground. Intracerebral hemorrhage (ICH, IH) is a stroke syndrome characterized by high mortality and disability rates. However, the apoptotic mechanisms underlying ICH remain incompletely understood. We employed bioinformatics methods to identify key apoptotic genes associated with ICH, thereby offering potential therapeutic targets and contributing to a deeper understanding of the pathogenic mechanisms of apoptosis in ICH. Materials and Methods. We retrieved microarray data of ICH patients from the Gene Expression Omnibus database and intersected it with apoptotic genes, filtering out apoptosis-related differentially expressed genes (ARDEGs). Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were conducted, and a logic regression-based diagnostic model for ICH was constructed. Key genes were identified through least absolute shrinkage and selection operator regression after model validation. Subsequently, mRNA-transcription factor (mRNA-TF), mRNA–microRNA (mRNA–miRNA), mRNA-RNA-binding protein (mRNA-RBP), and mRNA-drug regulatory networks were established, followed by immune infiltration analysis. Results. We identified 29 ARDEGs related to ICH, primarily participating in biological processes such as the extrinsic apoptotic signaling pathway and neuroinflammatory response. The ICH model confirmed PPP1R15A, IL1R1, and TYROBP as key genes with diagnostic and therapeutic value. Thirteen immune cell types showed a correlation with ICH; notably, activated dendritic cells and CD56dim natural killer cells exhibited significant correlations, and key genes were positively correlated with all 13 immune cell types. The regulatory networks (mRNA-TF, mRNA–miRNA, mRNA-RBP, and mRNA-drug) provided insights into the apoptotic gene regulation mechanisms and potential drugs in ICH. Conclusion. Through bioinformatics analysis, we identified PPP1R15A, IL1R1, and TYROBP as crucial genes in the occurrence and progression of ICH, with the potential involvement of Activated dendritic cells and CD56dim natural killer cells in these processes.http://dx.doi.org/10.1155/2024/6189986 |
| spellingShingle | Pandi Chen Gengfan Ye Jia Li Kuan Feng Guangyao Zhu Maosong Chen Wei Chen Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration Journal of Immunology Research |
| title | Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration |
| title_full | Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration |
| title_fullStr | Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration |
| title_full_unstemmed | Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration |
| title_short | Integrative Bioinformatics Analysis Unravel the Association between Intracerebral Hemorrhage and Cellular Apoptosis through Immune Infiltration |
| title_sort | integrative bioinformatics analysis unravel the association between intracerebral hemorrhage and cellular apoptosis through immune infiltration |
| url | http://dx.doi.org/10.1155/2024/6189986 |
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