Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry
Background: The efficiency of recombinant protein production in Yarrowia lipolytica is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Def...
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Elsevier
2025-01-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345824000290 |
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| author | Oliver Birrenbach Peter Czermak |
| author_facet | Oliver Birrenbach Peter Czermak |
| author_sort | Oliver Birrenbach |
| collection | DOAJ |
| description | Background: The efficiency of recombinant protein production in Yarrowia lipolytica is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Defined media also increase selectivity, reduce contamination risks, and enable precise nutrient control, leading to better growth and higher productivity. We used a design of experiments approach based on the elemental composition of yeast for the arithmetic development and optimization of a defined medium for the Y. lipolytica auxotrophic strain PO1f. Results: Statistically supported optimal concentrations of 10 g*L−1 glucose and 2.29 g*L−1 leucine enabled superior growth in the new in silico yeast (ISY) medium. Specific growth rates of 0.305 h−1 were achieved for the auxotrophic Yarrowia strain. Thiamine hydrochloride was a growth-limiting component, and higher concentrations increased the cell density of Y. lipolytica PO1f cultures by a factor of 30. Conclusions: ISY medium was suitable not only for the cultivation of Y. lipolytica but also universally applicable for Yarrowia-like and other yeasts, achieving better growth rates and yields compared to existing chemically defined media.How to cite: Birrenbach O, Czermak P. Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.10.001. |
| format | Article |
| id | doaj-art-70c81059f99d45f9b79bb86aa9527773 |
| institution | OA Journals |
| issn | 0717-3458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj-art-70c81059f99d45f9b79bb86aa95277732025-08-20T02:37:30ZengElsevierElectronic Journal of Biotechnology0717-34582025-01-0173323710.1016/j.ejbt.2024.10.001Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicryOliver Birrenbach0Peter Czermak1Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Central Hesse, Giessen, GermanyBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Central Hesse, Giessen, Germany; Faculty of Biology and Chemistry, Justus Liebig University, Giessen, Germany; Corresponding author.Background: The efficiency of recombinant protein production in Yarrowia lipolytica is strongly influenced by the culture medium. Complex media have limited applications because the composition is undefined and variable, whereas chemically defined media achieve better yields and reproducibility. Defined media also increase selectivity, reduce contamination risks, and enable precise nutrient control, leading to better growth and higher productivity. We used a design of experiments approach based on the elemental composition of yeast for the arithmetic development and optimization of a defined medium for the Y. lipolytica auxotrophic strain PO1f. Results: Statistically supported optimal concentrations of 10 g*L−1 glucose and 2.29 g*L−1 leucine enabled superior growth in the new in silico yeast (ISY) medium. Specific growth rates of 0.305 h−1 were achieved for the auxotrophic Yarrowia strain. Thiamine hydrochloride was a growth-limiting component, and higher concentrations increased the cell density of Y. lipolytica PO1f cultures by a factor of 30. Conclusions: ISY medium was suitable not only for the cultivation of Y. lipolytica but also universally applicable for Yarrowia-like and other yeasts, achieving better growth rates and yields compared to existing chemically defined media.How to cite: Birrenbach O, Czermak P. Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry. Electron J Biotechnol 2025;73. https://doi.org/10.1016/j.ejbt.2024.10.001.http://www.sciencedirect.com/science/article/pii/S0717345824000290AuxotrophyBiological mimicryChemically defined mediumElemental compositionIn silico yeast mediumLeucine |
| spellingShingle | Oliver Birrenbach Peter Czermak Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry Electronic Journal of Biotechnology Auxotrophy Biological mimicry Chemically defined medium Elemental composition In silico yeast medium Leucine |
| title | Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry |
| title_full | Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry |
| title_fullStr | Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry |
| title_full_unstemmed | Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry |
| title_short | Development of a chemically defined medium for Yarrowia yeasts using a strategy of biological mimicry |
| title_sort | development of a chemically defined medium for yarrowia yeasts using a strategy of biological mimicry |
| topic | Auxotrophy Biological mimicry Chemically defined medium Elemental composition In silico yeast medium Leucine |
| url | http://www.sciencedirect.com/science/article/pii/S0717345824000290 |
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