Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae
To further explore the biological roles of MoRei1 in the rice blast fungus Magnaporthe oryzae, we carried out MoREI1 gene deletion, phenotypic analysis of ΔMorei1 mutants, gene complementation and identification of its interaction proteins. The results showed that the ΔMorei1 mutants were defective...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2022-08-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.06.153 |
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| author | TANG Shuai XU Zhe LÜ Wuyun TONG Qi XIAO Yu WANG Zhengyi |
| author_facet | TANG Shuai XU Zhe LÜ Wuyun TONG Qi XIAO Yu WANG Zhengyi |
| author_sort | TANG Shuai |
| collection | DOAJ |
| description | To further explore the biological roles of MoRei1 in the rice blast fungus Magnaporthe oryzae, we carried out MoREI1 gene deletion, phenotypic analysis of ΔMorei1 mutants, gene complementation and identification of its interaction proteins. The results showed that the ΔMorei1 mutants were defective in sexual reproduction, appressorium formation and pathogenicity. Deletion of MoREI1 led to significant increase of sensitivity to cell wall damaging and oxidative stress agents, and delay of glycogen mobilization and degradation during appressorium development. Moreover, the MoREI1 gene could partially complement the defects of Saccharomyces cerevisiae Δrei1 mutants, indicating that MoRei1 is a functional homolog of yeast Rei1, and may share a similar role in ribosome biogenesis. MoRei1 physically interacts with MoAlb1, a predicted nuclear export factor. Interestingly, deletion of MoREI1 resulted in the impairment of nuclear localization of MoAlb1. These results lay a foundation to clarify the roles of MoRei1 in fungal morphogenesis and pathogenicity in M. oryzae. |
| format | Article |
| id | doaj-art-709e2da70ade47e88a4525414601564c |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-709e2da70ade47e88a4525414601564c2025-08-20T03:58:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552022-08-014844345210.3785/j.issn.1008-9209.2021.06.15310089209Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzaeTANG ShuaiXU ZheLÜ WuyunTONG QiXIAO YuWANG ZhengyiTo further explore the biological roles of MoRei1 in the rice blast fungus Magnaporthe oryzae, we carried out MoREI1 gene deletion, phenotypic analysis of ΔMorei1 mutants, gene complementation and identification of its interaction proteins. The results showed that the ΔMorei1 mutants were defective in sexual reproduction, appressorium formation and pathogenicity. Deletion of MoREI1 led to significant increase of sensitivity to cell wall damaging and oxidative stress agents, and delay of glycogen mobilization and degradation during appressorium development. Moreover, the MoREI1 gene could partially complement the defects of Saccharomyces cerevisiae Δrei1 mutants, indicating that MoRei1 is a functional homolog of yeast Rei1, and may share a similar role in ribosome biogenesis. MoRei1 physically interacts with MoAlb1, a predicted nuclear export factor. Interestingly, deletion of MoREI1 resulted in the impairment of nuclear localization of MoAlb1. These results lay a foundation to clarify the roles of MoRei1 in fungal morphogenesis and pathogenicity in M. oryzae.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.06.153<italic>Magnaporthe oryzae</italic> ribosome biogenesis factor MoRei1functional analysisMoAlb1 protein |
| spellingShingle | TANG Shuai XU Zhe LÜ Wuyun TONG Qi XIAO Yu WANG Zhengyi Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae 浙江大学学报. 农业与生命科学版 <italic>Magnaporthe oryzae</italic> ribosome biogenesis factor MoRei1 functional analysis MoAlb1 protein |
| title | Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae |
| title_full | Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae |
| title_fullStr | Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae |
| title_full_unstemmed | Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae |
| title_short | Functional analysis of the putative ribosome biogenesis factor MoRei1 in Magnaporthe oryzae |
| title_sort | functional analysis of the putative ribosome biogenesis factor morei1 in magnaporthe oryzae |
| topic | <italic>Magnaporthe oryzae</italic> ribosome biogenesis factor MoRei1 functional analysis MoAlb1 protein |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.06.153 |
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