Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia

Abstract Leptospirosis is a significant zoonotic disease that causes high economic losses in cattle production due to its association with abortions, stillbirths, infertility, and reduced milk yields. However, the epidemiology of bovine leptospirosis in Ethiopia is poorly understood. From October 20...

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Main Authors: Dereje Tulu Robi, Ararsa Bogale, Melkam Aleme, Beksisa Urge
Format: Article
Language:English
Published: BMC 2024-12-01
Series:BMC Veterinary Research
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Online Access:https://doi.org/10.1186/s12917-024-04418-9
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author Dereje Tulu Robi
Ararsa Bogale
Melkam Aleme
Beksisa Urge
author_facet Dereje Tulu Robi
Ararsa Bogale
Melkam Aleme
Beksisa Urge
author_sort Dereje Tulu Robi
collection DOAJ
description Abstract Leptospirosis is a significant zoonotic disease that causes high economic losses in cattle production due to its association with abortions, stillbirths, infertility, and reduced milk yields. However, the epidemiology of bovine leptospirosis in Ethiopia is poorly understood. From October 2020 to October 2021, a cross-sectional study was conducted to investigate the seroprevalence of serovar Hardjo in cattle in southwest Ethiopia, as well as the associated risk factors. To test for the existence of L. Hardjo antibodies, blood samples were taken from 461 cattle. Indirect ELISA was used to identify the presence of antibodies against L. Hardjo in sera samples. We conducted a multivariable random-effect logistic regression analysis to identify potential risk factors associated with L. Hardjo seropositivity. An overall L. Hardjo seroprevalence of 24.7% (95% CI: 20.2–48.8) and 53.5% (95% CI: 45.7–90.5) was recorded at the animal level and the herd level, respectively, in the study areas. This study revealed six factors influencing L. Hardjo seropositivity in cattle herds. Large herds had twice the odds of seropositivity (OR = 2.0, 95% CI: 1.1–3.8) compared to small herds. Co-grazing cattle exhibited higher odds (OR = 2.2, 95% CI: 1.2–4.1) of seropositivity. Extensive management systems significantly increased the odds (OR = 10.3, 95% CI: 1.7–61.8) compared to semi-intensive systems. Highland cattle had higher odds (OR = 3.7, 95% CI: 1.4–10.3) than lowland cattle. Older cattle (OR = 4.6, 95% CI: 2.4–8.9) were more likely to be seropositive. At the herd level, extensive management (OR = 2.8, 95% CI: 1.3–5.8) and large herds (OR = 2.5, 95% CI: 1.3–4.7) increased the risk of seropositivity. Herds with sheep/goats (OR = 2.3, 95% CI: 1.3–4.1) were also at higher risk, highlighting significant L. Hardjo seropositivity risk factors in cattle herds. The study findings showed that leptospirosis was highly prevalent across the study areas. As a result, use proper management, raise zoonotic awareness for leptospirosis, and conduct molecular bovine leptospirosis research in study areas were recommended.
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spelling doaj-art-7099d547e5be469bb2a2da9171fde5c52025-08-20T02:30:56ZengBMCBMC Veterinary Research1746-61482024-12-0120111210.1186/s12917-024-04418-9Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest EthiopiaDereje Tulu Robi0Ararsa Bogale1Melkam Aleme2Beksisa Urge3Ethiopian Institute of Agricultural Research, Tepi Agricultural Research CenterEthiopian Institute of Agricultural Research, Holeta Agricultural Research CenterEthiopian Institute of Agricultural Research, Tepi Agricultural Research CenterEthiopian Institute of Agricultural Research, Holeta Agricultural Research CenterAbstract Leptospirosis is a significant zoonotic disease that causes high economic losses in cattle production due to its association with abortions, stillbirths, infertility, and reduced milk yields. However, the epidemiology of bovine leptospirosis in Ethiopia is poorly understood. From October 2020 to October 2021, a cross-sectional study was conducted to investigate the seroprevalence of serovar Hardjo in cattle in southwest Ethiopia, as well as the associated risk factors. To test for the existence of L. Hardjo antibodies, blood samples were taken from 461 cattle. Indirect ELISA was used to identify the presence of antibodies against L. Hardjo in sera samples. We conducted a multivariable random-effect logistic regression analysis to identify potential risk factors associated with L. Hardjo seropositivity. An overall L. Hardjo seroprevalence of 24.7% (95% CI: 20.2–48.8) and 53.5% (95% CI: 45.7–90.5) was recorded at the animal level and the herd level, respectively, in the study areas. This study revealed six factors influencing L. Hardjo seropositivity in cattle herds. Large herds had twice the odds of seropositivity (OR = 2.0, 95% CI: 1.1–3.8) compared to small herds. Co-grazing cattle exhibited higher odds (OR = 2.2, 95% CI: 1.2–4.1) of seropositivity. Extensive management systems significantly increased the odds (OR = 10.3, 95% CI: 1.7–61.8) compared to semi-intensive systems. Highland cattle had higher odds (OR = 3.7, 95% CI: 1.4–10.3) than lowland cattle. Older cattle (OR = 4.6, 95% CI: 2.4–8.9) were more likely to be seropositive. At the herd level, extensive management (OR = 2.8, 95% CI: 1.3–5.8) and large herds (OR = 2.5, 95% CI: 1.3–4.7) increased the risk of seropositivity. Herds with sheep/goats (OR = 2.3, 95% CI: 1.3–4.1) were also at higher risk, highlighting significant L. Hardjo seropositivity risk factors in cattle herds. The study findings showed that leptospirosis was highly prevalent across the study areas. As a result, use proper management, raise zoonotic awareness for leptospirosis, and conduct molecular bovine leptospirosis research in study areas were recommended.https://doi.org/10.1186/s12917-024-04418-9Serovar HardjoZoonosisCattlePrevalenceRisk factorsEthiopia
spellingShingle Dereje Tulu Robi
Ararsa Bogale
Melkam Aleme
Beksisa Urge
Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia
BMC Veterinary Research
Serovar Hardjo
Zoonosis
Cattle
Prevalence
Risk factors
Ethiopia
title Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia
title_full Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia
title_fullStr Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia
title_full_unstemmed Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia
title_short Herd and animal level seroprevalence and associated risk factors of Leptospira interrogans sensu lato serovar Hardjo in cattle in southwest Ethiopia
title_sort herd and animal level seroprevalence and associated risk factors of leptospira interrogans sensu lato serovar hardjo in cattle in southwest ethiopia
topic Serovar Hardjo
Zoonosis
Cattle
Prevalence
Risk factors
Ethiopia
url https://doi.org/10.1186/s12917-024-04418-9
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