Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3
Gliclazide (GLZ), an oral antihyperglycemic medication, has additional beneficial effects, such as anti-inflammatory and antioxidant properties, besides lowering blood glucose levels. In this study, the radioprotective effect of GLZ was evaluated against ionizing radiation (IR)-induced intestinal in...
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Termedia Publishing House
2024-12-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Gliclazide-protects-ionizing-radiation-induced-intestinal-injury-r-nin-mice-by-inhibiting-oxidative-stress-and-caspase-3,85,55196,1,1.html |
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| author | Soroush Arzani Soghra Farzipour Fereshteh Talebpour Amiri Seyed Jalal Hosseinimehr |
| author_facet | Soroush Arzani Soghra Farzipour Fereshteh Talebpour Amiri Seyed Jalal Hosseinimehr |
| author_sort | Soroush Arzani |
| collection | DOAJ |
| description | Gliclazide (GLZ), an oral antihyperglycemic medication, has additional beneficial effects, such as anti-inflammatory and antioxidant properties, besides lowering blood glucose levels. In this study, the radioprotective effect of GLZ was evaluated against ionizing radiation (IR)-induced intestinal injury in mice. Eight groups of mice were randomized as follows: control, GLZ (5, 10, and 25 mg/kg), IR (6 Gy), and IR + GLZ (at 5, 10, and 25 mg/kg). GLZ was administered to the mice for eight consecutive days, after which they were exposed to X-rays at a single dose of 6 Gy. After irradiation, biochemical parameters, immunohistochemical, and histological examinations were conducted on the ileum of the mice. IR exposure increased the levels of malondialdehyde and protein carbonyl, while glutathione levels, as oxidative stress biomarkers, decreased. Apoptosis in ileum tissues was also assessed. Furthermore, histopathological changes were observed in the irradiated mice. GLZ treatment significantly mitigated these changes. The administration of GLZ resulted in a marked decrease in caspase-3 immunoreactivity in the ileum of irradiated mice. This preclinical study exhibited that GLZ has a radioprotective effect against intestinal injury by inhibiting oxidative stress and apoptosis. |
| format | Article |
| id | doaj-art-70972f02239046e78289b699a5d5a5e1 |
| institution | Kabale University |
| issn | 0860-7796 2353-9461 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Termedia Publishing House |
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| series | BioTechnologia |
| spelling | doaj-art-70972f02239046e78289b699a5d5a5e12025-01-27T11:25:46ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612024-12-01105436737610.5114/bta.2024.14525755196Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3Soroush ArzaniSoghra FarzipourFereshteh Talebpour AmiriSeyed Jalal HosseinimehrGliclazide (GLZ), an oral antihyperglycemic medication, has additional beneficial effects, such as anti-inflammatory and antioxidant properties, besides lowering blood glucose levels. In this study, the radioprotective effect of GLZ was evaluated against ionizing radiation (IR)-induced intestinal injury in mice. Eight groups of mice were randomized as follows: control, GLZ (5, 10, and 25 mg/kg), IR (6 Gy), and IR + GLZ (at 5, 10, and 25 mg/kg). GLZ was administered to the mice for eight consecutive days, after which they were exposed to X-rays at a single dose of 6 Gy. After irradiation, biochemical parameters, immunohistochemical, and histological examinations were conducted on the ileum of the mice. IR exposure increased the levels of malondialdehyde and protein carbonyl, while glutathione levels, as oxidative stress biomarkers, decreased. Apoptosis in ileum tissues was also assessed. Furthermore, histopathological changes were observed in the irradiated mice. GLZ treatment significantly mitigated these changes. The administration of GLZ resulted in a marked decrease in caspase-3 immunoreactivity in the ileum of irradiated mice. This preclinical study exhibited that GLZ has a radioprotective effect against intestinal injury by inhibiting oxidative stress and apoptosis.https://www.termedia.pl/Gliclazide-protects-ionizing-radiation-induced-intestinal-injury-r-nin-mice-by-inhibiting-oxidative-stress-and-caspase-3,85,55196,1,1.htmlgliclazide caspase-3 oxidative stress intestinal injury radioprotective ionizing radiation |
| spellingShingle | Soroush Arzani Soghra Farzipour Fereshteh Talebpour Amiri Seyed Jalal Hosseinimehr Gliclazide protects ionizing radiation-induced intestinal injury in mice by inhibiting oxidative stress and caspase-3 BioTechnologia gliclazide caspase-3 oxidative stress intestinal injury radioprotective ionizing radiation |
| title | Gliclazide protects ionizing radiation-induced intestinal injury
in mice by inhibiting oxidative stress and caspase-3 |
| title_full | Gliclazide protects ionizing radiation-induced intestinal injury
in mice by inhibiting oxidative stress and caspase-3 |
| title_fullStr | Gliclazide protects ionizing radiation-induced intestinal injury
in mice by inhibiting oxidative stress and caspase-3 |
| title_full_unstemmed | Gliclazide protects ionizing radiation-induced intestinal injury
in mice by inhibiting oxidative stress and caspase-3 |
| title_short | Gliclazide protects ionizing radiation-induced intestinal injury
in mice by inhibiting oxidative stress and caspase-3 |
| title_sort | gliclazide protects ionizing radiation induced intestinal injury in mice by inhibiting oxidative stress and caspase 3 |
| topic | gliclazide caspase-3 oxidative stress intestinal injury radioprotective ionizing radiation |
| url | https://www.termedia.pl/Gliclazide-protects-ionizing-radiation-induced-intestinal-injury-r-nin-mice-by-inhibiting-oxidative-stress-and-caspase-3,85,55196,1,1.html |
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