Ion-pair reversed-phase chromatography analysis of oligonucleotides using ultra-short (20 x 2.1 mm) columns. Tutorial

Ion-pair reversed-phase chromatography analysis of oligonucleotides using ultra-short (20 x 2.1 mm) columns. Tutorial

With this work, we present a comprehensive tutorial for the analysis of oligonucleotides (ONs, 5 to 100 mer) using ion-pair reversed-phase liquid chromatography (IP-RPLC) on ultra-short columns (20 × 2.1 mm). We explore the impact of ion-pairing (IP) agents on ON retention and demonstrate that while...

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Bibliographic Details
Main Authors: Szabolcs Fekete, Mateusz Imiołek, Matthew Lauber
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Journal of Chromatography Open
Subjects:
Oligonucleotide separation
Ion-pairing
Retention modeling
Ultra-short column
Platform method
Online Access:http://www.sciencedirect.com/science/article/pii/S2772391724000744
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