Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail
This study aimed to investigate the potential role of the vasoactive intestinal peptide receptor-1 (<i>VIPR-1</i>) gene polymorphisms and haplotypes in influencing egg production performance and egg quality parameters in laying-type quail. Genomic DNA was extracted from 150 quail across...
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2025-05-01
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| author | Xinle Wang Huricha Chen Ying Lei Qiankun Wang Gan Li Junyan Bai |
| author_facet | Xinle Wang Huricha Chen Ying Lei Qiankun Wang Gan Li Junyan Bai |
| author_sort | Xinle Wang |
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| description | This study aimed to investigate the potential role of the vasoactive intestinal peptide receptor-1 (<i>VIPR-1</i>) gene polymorphisms and haplotypes in influencing egg production performance and egg quality parameters in laying-type quail. Genomic DNA was extracted from 150 quail across three strains: Chinese yellow (CY, <i>n</i> = 50), Beijing white (BW, <i>n</i> = 50), and Korean (KO, <i>n</i> = 50). We designed two pairs of primers and initiated PCR amplification, after which the amplified products were sent to a testing company for purification. Sanger sequencing was employed to identify single nucleotide polymorphisms (SNPs) within the <i>VIPR-1</i> gene. Two SNP sites were selected for genotyping; g.1603402T>G was analyzed using PCR-RFLP with the <i>Bsr</i>D I enzyme, while g.1614884A>G was genotyped using the <i>Hpy</i>CH4 IV enzyme. The association results revealed that the g.1603402T>G site showed significant association with egg shell thickness (EST) in the BW strain (<i>p</i> < 0.05). There were no significant associations between these two loci and the remaining egg quality traits in the BW and KO strains (<i>p</i> > 0.05). Differences in egg quality and laying performance among haplotype combinations were not significant (<i>p</i> > 0.05). In conclusion, the <i>VIPR-1</i> gene, with its identified polymorphisms and haplotypes, has potential as a molecular marker that could improve egg shell thickness in BW quail. |
| format | Article |
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| institution | OA Journals |
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| publishDate | 2025-05-01 |
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| spelling | doaj-art-7062ebd97a87403c8f55cfe6b478a2cf2025-08-20T02:33:36ZengMDPI AGAnimals2076-26152025-05-011510137310.3390/ani15101373Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type QuailXinle Wang0Huricha Chen1Ying Lei2Qiankun Wang3Gan Li4Junyan Bai5College of Animal Science, Henan University of Science and Technology, Luoyang 471023, ChinaSchool of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaCollege of Animal Science, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Animal Science, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Animal Science, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Animal Science, Henan University of Science and Technology, Luoyang 471023, ChinaThis study aimed to investigate the potential role of the vasoactive intestinal peptide receptor-1 (<i>VIPR-1</i>) gene polymorphisms and haplotypes in influencing egg production performance and egg quality parameters in laying-type quail. Genomic DNA was extracted from 150 quail across three strains: Chinese yellow (CY, <i>n</i> = 50), Beijing white (BW, <i>n</i> = 50), and Korean (KO, <i>n</i> = 50). We designed two pairs of primers and initiated PCR amplification, after which the amplified products were sent to a testing company for purification. Sanger sequencing was employed to identify single nucleotide polymorphisms (SNPs) within the <i>VIPR-1</i> gene. Two SNP sites were selected for genotyping; g.1603402T>G was analyzed using PCR-RFLP with the <i>Bsr</i>D I enzyme, while g.1614884A>G was genotyped using the <i>Hpy</i>CH4 IV enzyme. The association results revealed that the g.1603402T>G site showed significant association with egg shell thickness (EST) in the BW strain (<i>p</i> < 0.05). There were no significant associations between these two loci and the remaining egg quality traits in the BW and KO strains (<i>p</i> > 0.05). Differences in egg quality and laying performance among haplotype combinations were not significant (<i>p</i> > 0.05). In conclusion, the <i>VIPR-1</i> gene, with its identified polymorphisms and haplotypes, has potential as a molecular marker that could improve egg shell thickness in BW quail.https://www.mdpi.com/2076-2615/15/10/1373<i>VIPR-1</i>polymorphismegg qualitylaying performancequail |
| spellingShingle | Xinle Wang Huricha Chen Ying Lei Qiankun Wang Gan Li Junyan Bai Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail Animals <i>VIPR-1</i> polymorphism egg quality laying performance quail |
| title | Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail |
| title_full | Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail |
| title_fullStr | Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail |
| title_full_unstemmed | Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail |
| title_short | Association of Novel Mutations in the Vasoactive Intestinal Peptide Receptor-1 Gene with Egg Shell Thickness in Three Strains of Laying-Type Quail |
| title_sort | association of novel mutations in the vasoactive intestinal peptide receptor 1 gene with egg shell thickness in three strains of laying type quail |
| topic | <i>VIPR-1</i> polymorphism egg quality laying performance quail |
| url | https://www.mdpi.com/2076-2615/15/10/1373 |
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