ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle
Phosphorylation post-translational modification plays an important role in postmortem muscle quality traits. Adenosine triphosphate (ATP) is an energy source and a key substrate of phosphorylation which provides the phosphatase groups to proteins in the presence of protein kinases. However, in postm...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311920633615 |
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| author | Chi REN Cheng-li HOU De-quan ZHANG Xin LI Xiong XIAO Yu-qiang BAI |
| author_facet | Chi REN Cheng-li HOU De-quan ZHANG Xin LI Xiong XIAO Yu-qiang BAI |
| author_sort | Chi REN |
| collection | DOAJ |
| description | Phosphorylation post-translational modification plays an important role in postmortem muscle quality traits. Adenosine triphosphate (ATP) is an energy source and a key substrate of phosphorylation which provides the phosphatase groups to proteins in the presence of protein kinases. However, in postmortem muscle, the effects of ATP content on phosphorylation are poorly studied. The study investigated the effect of ATP on protein phosphorylation and degradation in postmortem ovine muscle. The ground muscle with/without additional ATP were treated/control groups and stored at 25 and 4°C, respectively. The ATP content led to different changes of pH value between the ATP-treated and control groups. The phosphorylation level of myofibrillar proteins was higher (P<0.05) in ATP-treated group compared to the control group at both temperatures, which suggested that ATP played a vital role in postmortem protein phosphorylation. A slower degradation rate of µ-calpain, desmin and troponin T was observed in the ATP-treated group which showed that there was a negative relationship between ATP level and the degradation of proteins. These observations clearly highlighted the role of ATP on the development of meat quality by regulating the phosphorylation and degradation of myofibrillar proteins in postmortem ovine muscle. |
| format | Article |
| id | doaj-art-705e3ce9ecae4f14a3401c67cb753c5e |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-705e3ce9ecae4f14a3401c67cb753c5e2025-08-20T03:56:13ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192021-01-0120131131810.1016/S2095-3119(20)63361-5ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscleChi REN0Cheng-li HOU1De-quan ZHANG2Xin LI3Xiong XIAO4Yu-qiang BAI5Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.ChinaCorrespondence ZHANG De-quan, Tel/Fax: +86-10-62818740; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, P.R.ChinaPhosphorylation post-translational modification plays an important role in postmortem muscle quality traits. Adenosine triphosphate (ATP) is an energy source and a key substrate of phosphorylation which provides the phosphatase groups to proteins in the presence of protein kinases. However, in postmortem muscle, the effects of ATP content on phosphorylation are poorly studied. The study investigated the effect of ATP on protein phosphorylation and degradation in postmortem ovine muscle. The ground muscle with/without additional ATP were treated/control groups and stored at 25 and 4°C, respectively. The ATP content led to different changes of pH value between the ATP-treated and control groups. The phosphorylation level of myofibrillar proteins was higher (P<0.05) in ATP-treated group compared to the control group at both temperatures, which suggested that ATP played a vital role in postmortem protein phosphorylation. A slower degradation rate of µ-calpain, desmin and troponin T was observed in the ATP-treated group which showed that there was a negative relationship between ATP level and the degradation of proteins. These observations clearly highlighted the role of ATP on the development of meat quality by regulating the phosphorylation and degradation of myofibrillar proteins in postmortem ovine muscle.http://www.sciencedirect.com/science/article/pii/S2095311920633615ATPpostmortem ovine musclephosphorylationprotein degradation |
| spellingShingle | Chi REN Cheng-li HOU De-quan ZHANG Xin LI Xiong XIAO Yu-qiang BAI ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle Journal of Integrative Agriculture ATP postmortem ovine muscle phosphorylation protein degradation |
| title | ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle |
| title_full | ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle |
| title_fullStr | ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle |
| title_full_unstemmed | ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle |
| title_short | ATP regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle |
| title_sort | atp regulates the phosphorylation and degradation of myofibrillar proteins in ground ovine muscle |
| topic | ATP postmortem ovine muscle phosphorylation protein degradation |
| url | http://www.sciencedirect.com/science/article/pii/S2095311920633615 |
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