Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency
Objectives This research first investigated the effect of mesoporous silica nanoparticles (nMS) carrying chlorhexidine and silver (nMS-nAg-Chx) on periodontitis-related biofilms. This study aimed to investigate (1) the antibacterial activity on Porphyromonas gingivalis (P. gingivalis) biofilm; (2) t...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2361403 |
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| author | Lixin Fang Yishuang Zhang Long Cheng Hao Zheng Yiyi Wang Lu Qin Yingchun Cai Lei Cheng Wen Zhou Fei Liu Suping Wang |
| author_facet | Lixin Fang Yishuang Zhang Long Cheng Hao Zheng Yiyi Wang Lu Qin Yingchun Cai Lei Cheng Wen Zhou Fei Liu Suping Wang |
| author_sort | Lixin Fang |
| collection | DOAJ |
| description | Objectives This research first investigated the effect of mesoporous silica nanoparticles (nMS) carrying chlorhexidine and silver (nMS-nAg-Chx) on periodontitis-related biofilms. This study aimed to investigate (1) the antibacterial activity on Porphyromonas gingivalis (P. gingivalis) biofilm; (2) the suppressing effect on virulence of P. gingivalis biofilm; (3) the regulating effect on periodontitis-related multispecies biofilm.Methods Silver nanoparticles (nAg) and chlorhexidine (Chx) were co-loaded into nMS to form nMS-nAg-Chx. Inhibitory zone test and minimum inhibitory concentration (MIC) against P. gingivalis were tested. Growth curves, crystal violet (CV) staining, live/dead staining and scanning electron microscopy (SEM) observation were performed. Biofilm virulence was assessed. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and Quantitative Real Time-PCR (qPCR) were performed to validate the activity and composition changes of multispecies biofilm (P. gingivalis, Streptococcus gordonii and Streptococcus sanguinis).Results nMS-nAg-Chx inhibited P. gingivalis biofilm dose-dependently (p<0.05), with MIC of 18.75 µg/mL. There were fewer live bacteria, less biomass and less virulence in nMS-nAg-Chx groups (p<0.05). nMS-nAg-Chx inhibited and modified periodontitis-related biofilms. The proportion of pathogenic bacteria decreased from 16.08 to 1.07% and that of helpful bacteria increased from 82.65 to 94.31% in 25 μg/mL nMS-nAg-Chx group for 72 h.Conclusions nMS-nAg-Chx inhibited P. gingivalis growth, decreased biofilm virulence and modulated periodontitis-related multispecies biofilms toward healthy tendency. pH-sensitive nMS-nAg-Chx inhibit the pathogens and regulate oral microecology, showing great potential in periodontitis adjunctive therapy. |
| format | Article |
| id | doaj-art-705ab3650f2c455e9d5286c42ebb01b4 |
| institution | OA Journals |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-705ab3650f2c455e9d5286c42ebb01b42025-08-20T02:23:09ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2361403Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendencyLixin Fang0Yishuang Zhang1Long Cheng2Hao Zheng3Yiyi Wang4Lu Qin5Yingchun Cai6Lei Cheng7Wen Zhou8Fei Liu9Suping Wang10Stomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, ChinaSchool and Hospital of Stomatology, Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, Fuzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaStomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaObjectives This research first investigated the effect of mesoporous silica nanoparticles (nMS) carrying chlorhexidine and silver (nMS-nAg-Chx) on periodontitis-related biofilms. This study aimed to investigate (1) the antibacterial activity on Porphyromonas gingivalis (P. gingivalis) biofilm; (2) the suppressing effect on virulence of P. gingivalis biofilm; (3) the regulating effect on periodontitis-related multispecies biofilm.Methods Silver nanoparticles (nAg) and chlorhexidine (Chx) were co-loaded into nMS to form nMS-nAg-Chx. Inhibitory zone test and minimum inhibitory concentration (MIC) against P. gingivalis were tested. Growth curves, crystal violet (CV) staining, live/dead staining and scanning electron microscopy (SEM) observation were performed. Biofilm virulence was assessed. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and Quantitative Real Time-PCR (qPCR) were performed to validate the activity and composition changes of multispecies biofilm (P. gingivalis, Streptococcus gordonii and Streptococcus sanguinis).Results nMS-nAg-Chx inhibited P. gingivalis biofilm dose-dependently (p<0.05), with MIC of 18.75 µg/mL. There were fewer live bacteria, less biomass and less virulence in nMS-nAg-Chx groups (p<0.05). nMS-nAg-Chx inhibited and modified periodontitis-related biofilms. The proportion of pathogenic bacteria decreased from 16.08 to 1.07% and that of helpful bacteria increased from 82.65 to 94.31% in 25 μg/mL nMS-nAg-Chx group for 72 h.Conclusions nMS-nAg-Chx inhibited P. gingivalis growth, decreased biofilm virulence and modulated periodontitis-related multispecies biofilms toward healthy tendency. pH-sensitive nMS-nAg-Chx inhibit the pathogens and regulate oral microecology, showing great potential in periodontitis adjunctive therapy.https://www.tandfonline.com/doi/10.1080/20002297.2024.2361403Mesoporous silica nanoparticlesdrug delivery systemPorphyromonas gingivalisantibacterialantibiofilmperiodontitis |
| spellingShingle | Lixin Fang Yishuang Zhang Long Cheng Hao Zheng Yiyi Wang Lu Qin Yingchun Cai Lei Cheng Wen Zhou Fei Liu Suping Wang Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency Journal of Oral Microbiology Mesoporous silica nanoparticles drug delivery system Porphyromonas gingivalis antibacterial antibiofilm periodontitis |
| title | Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency |
| title_full | Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency |
| title_fullStr | Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency |
| title_full_unstemmed | Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency |
| title_short | Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency |
| title_sort | silica nanoparticles containing nano silver and chlorhexidine to suppress porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency |
| topic | Mesoporous silica nanoparticles drug delivery system Porphyromonas gingivalis antibacterial antibiofilm periodontitis |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2361403 |
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