Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia.
Objective: The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes. Mate...
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Network for the Veterinarians of Bangladesh
2025-03-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=196560 |
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| author | Amirudin Dg Malewa Rusdin Rusdin Mardiah Mangun Isyana Khaerunnisa Dwi Lestari Yulius Duma. |
| author_facet | Amirudin Dg Malewa Rusdin Rusdin Mardiah Mangun Isyana Khaerunnisa Dwi Lestari Yulius Duma. |
| author_sort | Amirudin Dg Malewa |
| collection | DOAJ |
| description | Objective: The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes.
Materials and Methods: This study used 39 Napu buffaloes (9 males and 30 females). Morphometric measurements were performed using a measuring tape. The GH and IGF-1 gene diversity analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques.
Results: The results showed that there is diversity in BW and body size of Napu buffaloes due to the influence of age and sex. The results of PCR-RFLP analysis of the GH|AluI gene showed 0% VV, 100% LL, and 0% VL genotypes. While the IGF-1|SnaBI gene shows the genotype AA 0%, BB 100%, and AB 0%. The sequencing results of the GH and IGF-1 genes in Napu buffaloes did not find mutations.
Conclusion: Age and sex in Napu buffaloes may affect BW and body size. The GH and IGF-1 genes in Napu buffaloes are monomorphic, so no association can be made with the morphometric parameters of Napu buffaloes. [J Adv Vet Anim Res 2025; 12(1.000): 205-213] |
| format | Article |
| id | doaj-art-705a5bafdc6c4b27ba783868ced0d576 |
| institution | OA Journals |
| issn | 2311-7710 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj-art-705a5bafdc6c4b27ba783868ced0d5762025-08-20T02:29:42ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102025-03-0112120521310.5455/javar.2025.l887196560Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia.Amirudin Dg Malewa0Rusdin Rusdin1Mardiah Mangun2Isyana Khaerunnisa3Dwi Lestari4Yulius Duma.5Department of Animal Husbandry, Faculty of Animal Science and Fisheries, Tadulako University, Palu 94119, Indonesia Department of Animal Husbandry, Faculty of Animal Science and Fisheries, Tadulako University, Palu 94119, Indonesia Department of Animal Husbandry, Faculty of Animal Science and Fisheries, Tadulako University, Palu 94119, Indonesia Research Center for Applied Zoology, National Research and Innovation Agency, Bogor 16911, Indonesia Research Center for Applied Zoology, National Research and Innovation Agency, Bogor 16911, Indonesia Department of Animal Husbandry, Faculty of Animal Science and Fisheries, Tadulako University, Palu 94119, Indonesia.Objective: The objectives of this study are to measure the body weight (BW) and morphometric parameters of Napu buffaloes, identify the growth hormone (GH) and insulin-like growth factor-1 (IGF-1) gene polymorphisms, and associate them with the BW and morphometric parameters of Napu buffaloes. Materials and Methods: This study used 39 Napu buffaloes (9 males and 30 females). Morphometric measurements were performed using a measuring tape. The GH and IGF-1 gene diversity analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing techniques. Results: The results showed that there is diversity in BW and body size of Napu buffaloes due to the influence of age and sex. The results of PCR-RFLP analysis of the GH|AluI gene showed 0% VV, 100% LL, and 0% VL genotypes. While the IGF-1|SnaBI gene shows the genotype AA 0%, BB 100%, and AB 0%. The sequencing results of the GH and IGF-1 genes in Napu buffaloes did not find mutations. Conclusion: Age and sex in Napu buffaloes may affect BW and body size. The GH and IGF-1 genes in Napu buffaloes are monomorphic, so no association can be made with the morphometric parameters of Napu buffaloes. [J Adv Vet Anim Res 2025; 12(1.000): 205-213]http://www.ejmanager.com/fulltextpdf.php?mno=196560igf-1; gh; morphometric; napu buffalo; polymorphism |
| spellingShingle | Amirudin Dg Malewa Rusdin Rusdin Mardiah Mangun Isyana Khaerunnisa Dwi Lestari Yulius Duma. Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia. Journal of Advanced Veterinary and Animal Research igf-1; gh; morphometric; napu buffalo; polymorphism |
| title | Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia. |
| title_full | Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia. |
| title_fullStr | Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia. |
| title_full_unstemmed | Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia. |
| title_short | Morphometric traits and identification of GH and IGF-1 gene polymorphisms in Napu buffalo (Bubalus bubalis) from Central Sulawesi, Indonesia. |
| title_sort | morphometric traits and identification of gh and igf 1 gene polymorphisms in napu buffalo bubalus bubalis from central sulawesi indonesia |
| topic | igf-1; gh; morphometric; napu buffalo; polymorphism |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=196560 |
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