The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation
Rafflesia is an endangered endophytic holoparasitic plant that lives the majority of its life inside the tissues of its sole plant host, Tetrastigma. Rafflesia floral buds emerge to produce the world’s largest single flower. Like other plants, holoparasites harbor a diverse microbiome, the role(s) o...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Plant Interactions |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2024.2304221 |
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| author | Jeanmaire Molina Roche C. de Guzman Adhityo Wicaksono Theodore Muth Ronniel Pedales Denia Diaz Ali Budhi Kusuma Chloe Li Hudson Margolis Feruza Karnitskiy Alysa Estopace Patricia Atanelov Max Bukhbinder Danilo Tandang John Rey Callado Joseph W. Morin Ian Fontanilla Destiny Davis Stephen Jones Mick Erickson James Adams Kyle Wallick David Kidwell-Slak Ari Novy Susan Pell |
| author_facet | Jeanmaire Molina Roche C. de Guzman Adhityo Wicaksono Theodore Muth Ronniel Pedales Denia Diaz Ali Budhi Kusuma Chloe Li Hudson Margolis Feruza Karnitskiy Alysa Estopace Patricia Atanelov Max Bukhbinder Danilo Tandang John Rey Callado Joseph W. Morin Ian Fontanilla Destiny Davis Stephen Jones Mick Erickson James Adams Kyle Wallick David Kidwell-Slak Ari Novy Susan Pell |
| author_sort | Jeanmaire Molina |
| collection | DOAJ |
| description | Rafflesia is an endangered endophytic holoparasitic plant that lives the majority of its life inside the tissues of its sole plant host, Tetrastigma. Rafflesia floral buds emerge to produce the world’s largest single flower. Like other plants, holoparasites harbor a diverse microbiome, the role(s) of which has remained largely unstudied. We characterized the bacterial microbiome of seeds of Rafflesia speciosa and cuttings of its host. We found evidence that R. speciosa seed has similar bacterial profiles to its infected host, which suggests that seeds sequester certain host bacteria, as well as acquire unique bacterial taxa from biotic associates of the fruit. We did not find evidence of mycorrhizal taxa in the microbiome. This is the first study of the microbial endophytes associated with any Rafflesia species and its host, a tripartite holobiont, and provides insights on its cryptic microbial partners. We discuss how this may aid horticultural propagation of Rafflesia. |
| format | Article |
| id | doaj-art-70552be6803044288a7b9194bd5eeacd |
| institution | OA Journals |
| issn | 1742-9145 1742-9153 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj-art-70552be6803044288a7b9194bd5eeacd2025-08-20T02:01:00ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532024-12-0119110.1080/17429145.2024.2304221The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivationJeanmaire Molina0Roche C. de Guzman1Adhityo Wicaksono2Theodore Muth3Ronniel Pedales4Denia Diaz5Ali Budhi Kusuma6Chloe Li7Hudson Margolis8Feruza Karnitskiy9Alysa Estopace10Patricia Atanelov11Max Bukhbinder12Danilo Tandang13John Rey Callado14Joseph W. Morin15Ian Fontanilla16Destiny Davis17Stephen Jones18Mick Erickson19James Adams20Kyle Wallick21David Kidwell-Slak22Ari Novy23Susan Pell24Biology Department, Pace University, New York, NY, USABioengineering Program, Department of Engineering, Hofstra University, Hempstead, NY, USADivision of Biotechnology, Genbinesia Foundation, Gresik, IndonesiaBrooklyn College, City University of New York, Brooklyn, USAInstitute of Biology, University of the Philippines Diliman, Quezon City, PhilippinesBiology Department, Pace University, New York, NY, USAIndonesian Centre for Extremophile Bioresources and Biotechnology (ICEBB), Sumbawa University of Technology, Sumbawa, IndonesiaUrban Barcode Research Program, DNA Learning Center, Cold Spring Harbor, NY, USAUrban Barcode Research Program, DNA Learning Center, Cold Spring Harbor, NY, USADepartment of Life Sciences, Long Island University, Brooklyn, NY, USAInstitute of Biology, University of the Philippines Diliman, Quezon City, PhilippinesDepartment of Life Sciences, Long Island University, Brooklyn, NY, USADepartment of Life Sciences, Long Island University, Brooklyn, NY, USAPhilippine National Museum, Manila, PhilippinesDepartment of Science and Technology-Forest Products Research and Development Institute, Laguna, PhilippinesDepartment of Life Sciences, Long Island University, Brooklyn, NY, USAInstitute of Biology, University of the Philippines Diliman, Quezon City, PhilippinesU.S. Botanic Garden, Washington, DC, USAU.S. Botanic Garden, Washington, DC, USAU.S. Botanic Garden, Washington, DC, USAU.S. Botanic Garden, Washington, DC, USAU.S. Botanic Garden, Washington, DC, USAU.S. Botanic Garden, Washington, DC, USASan Diego Botanic Garden, Encinitas, CA, USAU.S. Botanic Garden, Washington, DC, USARafflesia is an endangered endophytic holoparasitic plant that lives the majority of its life inside the tissues of its sole plant host, Tetrastigma. Rafflesia floral buds emerge to produce the world’s largest single flower. Like other plants, holoparasites harbor a diverse microbiome, the role(s) of which has remained largely unstudied. We characterized the bacterial microbiome of seeds of Rafflesia speciosa and cuttings of its host. We found evidence that R. speciosa seed has similar bacterial profiles to its infected host, which suggests that seeds sequester certain host bacteria, as well as acquire unique bacterial taxa from biotic associates of the fruit. We did not find evidence of mycorrhizal taxa in the microbiome. This is the first study of the microbial endophytes associated with any Rafflesia species and its host, a tripartite holobiont, and provides insights on its cryptic microbial partners. We discuss how this may aid horticultural propagation of Rafflesia.https://www.tandfonline.com/doi/10.1080/17429145.2024.2304221HolobiontholoparasiteOrobanchephhytobiomeTetrastigma |
| spellingShingle | Jeanmaire Molina Roche C. de Guzman Adhityo Wicaksono Theodore Muth Ronniel Pedales Denia Diaz Ali Budhi Kusuma Chloe Li Hudson Margolis Feruza Karnitskiy Alysa Estopace Patricia Atanelov Max Bukhbinder Danilo Tandang John Rey Callado Joseph W. Morin Ian Fontanilla Destiny Davis Stephen Jones Mick Erickson James Adams Kyle Wallick David Kidwell-Slak Ari Novy Susan Pell The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation Journal of Plant Interactions Holobiont holoparasite Orobanche phhytobiome Tetrastigma |
| title | The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation |
| title_full | The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation |
| title_fullStr | The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation |
| title_full_unstemmed | The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation |
| title_short | The endophyte’s endophytes: the microbial partners of the endangered plant parasite Rafflesia speciosa (Rafflesiaceae) reveal clues about its cryptic biology and cues for cultivation |
| title_sort | endophyte s endophytes the microbial partners of the endangered plant parasite rafflesia speciosa rafflesiaceae reveal clues about its cryptic biology and cues for cultivation |
| topic | Holobiont holoparasite Orobanche phhytobiome Tetrastigma |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2024.2304221 |
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