Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse
Background and Aim. Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. We aim to investigate the efficacy of ADMSCs transplantation in the acute liver failure (ALF) caused by carbon tetrachlorid...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/103643 |
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| author | Lisi Deng Guangze Liu Xin Wu Yaping Wang Minghua Tong Bing Liu Kuidong Wang Yuting Peng Xiangping Kong |
| author_facet | Lisi Deng Guangze Liu Xin Wu Yaping Wang Minghua Tong Bing Liu Kuidong Wang Yuting Peng Xiangping Kong |
| author_sort | Lisi Deng |
| collection | DOAJ |
| description | Background and Aim. Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. We aim to investigate the efficacy of ADMSCs transplantation in the acute liver failure (ALF) caused by carbon tetrachloride (CCl4) in mice. Methods. ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. ALF models were established by infusion of CCl4 and divided into two groups: control group; EGFP-ADMSCs transplantation group. The restoration of biological functions of the livers receiving transplantation was assessed via a variety of approaches such as survival rates, live function parameters, histological localization of EGFP-ADMSCs, and Immunofluorescence analysis. Results. ADMSCs were positive for CD105, CD44 but negative for CD45, CD34 and had adipogenic, osteogenic differentiation potential. The survival rate of transplantation group significantly increased compared to PBS group. Furthermore, the transplanted cells were well integrated into injured livers and produced albumin, cytokeratin-18. Conclusion. Direct transplantation of ADMSCs is an effective treatment for ALF. The transplanted ADMSCs exhibit the potential to differentiate into hepatocyte-like cells in the injured livers. |
| format | Article |
| id | doaj-art-70523c9e95fb4902bb1a7878d5cafa3a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-70523c9e95fb4902bb1a7878d5cafa3a2025-02-03T07:25:40ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/103643103643Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in MouseLisi Deng0Guangze Liu1Xin Wu2Yaping Wang3Minghua Tong4Bing Liu5Kuidong Wang6Yuting Peng7Xiangping Kong8Center of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaGuangzhou Eight People’s Hospital, Guangdong 510060, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaCenter of Infectious Diseases, 458th Hospital of PLA, No. 801 Dongfengdong Road, Guangzhou, Guangdong 510600, ChinaBackground and Aim. Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. We aim to investigate the efficacy of ADMSCs transplantation in the acute liver failure (ALF) caused by carbon tetrachloride (CCl4) in mice. Methods. ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. ALF models were established by infusion of CCl4 and divided into two groups: control group; EGFP-ADMSCs transplantation group. The restoration of biological functions of the livers receiving transplantation was assessed via a variety of approaches such as survival rates, live function parameters, histological localization of EGFP-ADMSCs, and Immunofluorescence analysis. Results. ADMSCs were positive for CD105, CD44 but negative for CD45, CD34 and had adipogenic, osteogenic differentiation potential. The survival rate of transplantation group significantly increased compared to PBS group. Furthermore, the transplanted cells were well integrated into injured livers and produced albumin, cytokeratin-18. Conclusion. Direct transplantation of ADMSCs is an effective treatment for ALF. The transplanted ADMSCs exhibit the potential to differentiate into hepatocyte-like cells in the injured livers.http://dx.doi.org/10.1155/2014/103643 |
| spellingShingle | Lisi Deng Guangze Liu Xin Wu Yaping Wang Minghua Tong Bing Liu Kuidong Wang Yuting Peng Xiangping Kong Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse The Scientific World Journal |
| title | Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse |
| title_full | Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse |
| title_fullStr | Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse |
| title_full_unstemmed | Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse |
| title_short | Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse |
| title_sort | adipose derived mesenchymal stem cells efficiently rescue carbon tetrachloride induced acute liver failure in mouse |
| url | http://dx.doi.org/10.1155/2014/103643 |
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