Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition
During the last few decades, the use of nanoparticles due to their unique properties, biodegradability, biocompatibility, and nanometer size to reduce the side effects of drugs, especially in cancer treatment, has been considered. Chitosan nanoparticles, as biopolymers, are known as drug carriers du...
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Elsevier
2025-03-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625000980 |
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| author | Parisa Rahimi Ensieh Ghasemi Davoud Esmaeili Malak Hekmati |
| author_facet | Parisa Rahimi Ensieh Ghasemi Davoud Esmaeili Malak Hekmati |
| author_sort | Parisa Rahimi |
| collection | DOAJ |
| description | During the last few decades, the use of nanoparticles due to their unique properties, biodegradability, biocompatibility, and nanometer size to reduce the side effects of drugs, especially in cancer treatment, has been considered. Chitosan nanoparticles, as biopolymers, are known as drug carriers due to their anti-bacterial and anti-cancer properties and for passing through the membrane barrier of cells. Also, Ficus carica sap has high amounts of polyphenol compounds, which can be a valuable source of antioxidants. In the present study, Chitosan-ficus carica sap nanocomposite was synthesized and The structural properties and morphology of functionalized Nano-chitosan were investigated using FTIR, SEM, and TEM analysis. In GC–MS chromatogram of alcoholic extract of Ficus carica sap, It shows the identified compounds in Ficus carica sap and the percentage of each compound. A total of 28 compounds were identified. Among the identified compounds, C11H13NO2 by 17.40 %, and C13H22O3 by 12.56 % had the highest amount compared to other compounds.The MTT test was carried out with different compound concentrations (12.5–25–50-6.25-3.12-1.56-0.78-0.39-0.19-0.009-04 μg/mL) and the MTT concentration was determined against the Hela cell line. The Hela cell line was treated with MTT concentration, and the amount of apoptosis was investigated by Flowcytometry technique. Also, MIC, MBC, and disk diffusion tests were performed for study compound antibacterial activity against major Gram-positive and Gram-negative bacteria according to CLSI2022. The cells were treated with an MTT concentration of 0.8 μg/mL with chitosan nanoparticles functionalized with Ficus carica sap. In this study, Chitosan-ficus carica sap nanocomposite the MIC and MBC against Gram-positive and Gram-negative bacteria determined 200 μg/mL. In the treatment group, apoptosis was determined 46.91 % and In the untreated group, apoptosis was 10.66 %. the results indicated apoptosis after treatment increased 4.4 times.Due to the effect of this nanocomposite compound in low concentrations against gram-positive and gram-negative bacteria inhibition and the property of inducing apoptosis in Hela cell lines, it is possible to research and use this compound as a therapeutic and preventive medicine against vaginal infections and cervical cancer. |
| format | Article |
| id | doaj-art-704d30030e864407b9aff624a72be4e9 |
| institution | DOAJ |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Results in Chemistry |
| spelling | doaj-art-704d30030e864407b9aff624a72be4e92025-08-20T02:56:51ZengElsevierResults in Chemistry2211-71562025-03-011410211510.1016/j.rechem.2025.102115Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibitionParisa Rahimi0Ensieh Ghasemi1Davoud Esmaeili2Malak Hekmati3Department of Nano Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, IranDepartment of Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, IranDepartment of Microbiology and Applied Virology Research Center Baqiyatallah University of Medical Sciences, Tehran, IranDepartment of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; Corresponding author.During the last few decades, the use of nanoparticles due to their unique properties, biodegradability, biocompatibility, and nanometer size to reduce the side effects of drugs, especially in cancer treatment, has been considered. Chitosan nanoparticles, as biopolymers, are known as drug carriers due to their anti-bacterial and anti-cancer properties and for passing through the membrane barrier of cells. Also, Ficus carica sap has high amounts of polyphenol compounds, which can be a valuable source of antioxidants. In the present study, Chitosan-ficus carica sap nanocomposite was synthesized and The structural properties and morphology of functionalized Nano-chitosan were investigated using FTIR, SEM, and TEM analysis. In GC–MS chromatogram of alcoholic extract of Ficus carica sap, It shows the identified compounds in Ficus carica sap and the percentage of each compound. A total of 28 compounds were identified. Among the identified compounds, C11H13NO2 by 17.40 %, and C13H22O3 by 12.56 % had the highest amount compared to other compounds.The MTT test was carried out with different compound concentrations (12.5–25–50-6.25-3.12-1.56-0.78-0.39-0.19-0.009-04 μg/mL) and the MTT concentration was determined against the Hela cell line. The Hela cell line was treated with MTT concentration, and the amount of apoptosis was investigated by Flowcytometry technique. Also, MIC, MBC, and disk diffusion tests were performed for study compound antibacterial activity against major Gram-positive and Gram-negative bacteria according to CLSI2022. The cells were treated with an MTT concentration of 0.8 μg/mL with chitosan nanoparticles functionalized with Ficus carica sap. In this study, Chitosan-ficus carica sap nanocomposite the MIC and MBC against Gram-positive and Gram-negative bacteria determined 200 μg/mL. In the treatment group, apoptosis was determined 46.91 % and In the untreated group, apoptosis was 10.66 %. the results indicated apoptosis after treatment increased 4.4 times.Due to the effect of this nanocomposite compound in low concentrations against gram-positive and gram-negative bacteria inhibition and the property of inducing apoptosis in Hela cell lines, it is possible to research and use this compound as a therapeutic and preventive medicine against vaginal infections and cervical cancer.http://www.sciencedirect.com/science/article/pii/S2211715625000980Chitosan nanoparticlesFicus carica sapApoptosisAnti-bacterialCervical CancerHuman papillomavirus |
| spellingShingle | Parisa Rahimi Ensieh Ghasemi Davoud Esmaeili Malak Hekmati Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition Results in Chemistry Chitosan nanoparticles Ficus carica sap Apoptosis Anti-bacterial Cervical Cancer Human papillomavirus |
| title | Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition |
| title_full | Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition |
| title_fullStr | Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition |
| title_full_unstemmed | Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition |
| title_short | Synthesis of Ficus carica sap-functionalized Nano-chitosan for biomedical applications: Apoptosis induction and tumor cell line growth inhibition |
| title_sort | synthesis of ficus carica sap functionalized nano chitosan for biomedical applications apoptosis induction and tumor cell line growth inhibition |
| topic | Chitosan nanoparticles Ficus carica sap Apoptosis Anti-bacterial Cervical Cancer Human papillomavirus |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625000980 |
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