The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust
This study was aimed at investigating the effects of Eucheuma cottonii (EC) in oxidative stress and the signaling for mucin synthesis in rat lungs chronically exposed to coal dust. Coal dust with concomitant oral administration of ethanolic extract of EC at doses of 150 (EC150) or 300 mg/kg BW (EC30...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2013/528146 |
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| author | Nia Kania Elly Mayangsari Bambang Setiawan Dian Nugrahenny Frans Tony Endang Sri Wahyuni M. Aris Widodo |
| author_facet | Nia Kania Elly Mayangsari Bambang Setiawan Dian Nugrahenny Frans Tony Endang Sri Wahyuni M. Aris Widodo |
| author_sort | Nia Kania |
| collection | DOAJ |
| description | This study was aimed at investigating the effects of Eucheuma cottonii (EC) in oxidative stress and the signaling for mucin synthesis in rat lungs chronically exposed to coal dust. Coal dust with concomitant oral administration of ethanolic extract of EC at doses of 150 (EC150) or 300 mg/kg BW (EC300) compared to exposed to PM10 coal dust at doses of 6.25 (CD6.25), 12.5 (CD12.5), or 25 mg/m3 (CD25) (an hour daily for 6 months) and nonexposure group (control). The malondialdehyde (MDA), epidermal growth factor (EGF), transforming growth factor (TGF)-α, epidermal growth factor receptor (EGFR), and MUC5AC levels were determined in the lung. The administration of EC300 significantly (p < 0.05) reduced the MDA levels in groups exposed to all doses of coal dust compared to the respective coal dust-exposed nonsupplemented groups. Although not statistically significant,EC reduced the EGF levels and EGFR expressions in CD12.5 and CD25 groups and decreased the TGF-α, level and MUC5AC expression in CD25 group compared to the respective coal dust-exposed nonsupplemented groups. EC was able to decrease oxidative stress and was also able to decrease signaling for mucin synthesis, at least a part, via reducing the ligand in chronic coal dust exposure. |
| format | Article |
| id | doaj-art-704cab5e912f470cb04aacb6ee89998a |
| institution | OA Journals |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | Journal of Toxicology |
| spelling | doaj-art-704cab5e912f470cb04aacb6ee89998a2025-08-20T02:06:46ZengWileyJournal of Toxicology1687-81911687-82052013-01-01201310.1155/2013/528146528146The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal DustNia Kania0Elly Mayangsari1Bambang Setiawan2Dian Nugrahenny3Frans Tony4Endang Sri Wahyuni5M. Aris Widodo6Department of Pathology, Ulin General Hospital, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin,South Kalimantan, IndonesiaLaboratory of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, IndonesiaDepartment of Medical Chemistry and Biochemistry, Faculty of Medicine, University of Lambung Mangkurat, Banjarmasin, South Kalimantan, IndonesiaLaboratory of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, IndonesiaDepartment of Marine, Faculty of Fisheries, University of Lambung Mangkurat, Banjarbaru, South Kalimantan, IndonesiaLaboratory of Physiology, Faculty of Medicine, Brawijaya University, Malang, East Java, IndonesiaLaboratory of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, IndonesiaThis study was aimed at investigating the effects of Eucheuma cottonii (EC) in oxidative stress and the signaling for mucin synthesis in rat lungs chronically exposed to coal dust. Coal dust with concomitant oral administration of ethanolic extract of EC at doses of 150 (EC150) or 300 mg/kg BW (EC300) compared to exposed to PM10 coal dust at doses of 6.25 (CD6.25), 12.5 (CD12.5), or 25 mg/m3 (CD25) (an hour daily for 6 months) and nonexposure group (control). The malondialdehyde (MDA), epidermal growth factor (EGF), transforming growth factor (TGF)-α, epidermal growth factor receptor (EGFR), and MUC5AC levels were determined in the lung. The administration of EC300 significantly (p < 0.05) reduced the MDA levels in groups exposed to all doses of coal dust compared to the respective coal dust-exposed nonsupplemented groups. Although not statistically significant,EC reduced the EGF levels and EGFR expressions in CD12.5 and CD25 groups and decreased the TGF-α, level and MUC5AC expression in CD25 group compared to the respective coal dust-exposed nonsupplemented groups. EC was able to decrease oxidative stress and was also able to decrease signaling for mucin synthesis, at least a part, via reducing the ligand in chronic coal dust exposure.http://dx.doi.org/10.1155/2013/528146 |
| spellingShingle | Nia Kania Elly Mayangsari Bambang Setiawan Dian Nugrahenny Frans Tony Endang Sri Wahyuni M. Aris Widodo The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust Journal of Toxicology |
| title | The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust |
| title_full | The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust |
| title_fullStr | The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust |
| title_full_unstemmed | The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust |
| title_short | The Effects of Eucheuma cottonii on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust |
| title_sort | effects of eucheuma cottonii on signaling pathway inducing mucin synthesis in rat lungs chronically exposed to particulate matter 10 pm10 coal dust |
| url | http://dx.doi.org/10.1155/2013/528146 |
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