Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection
The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework (MOF) was synthesized and applied in loop-mediated isothermal amplification (named Cu-TCPP@LAMP), which can inhibit the no...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000319 |
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| author | Jiale Gao Xiaodong Sun Yongxin Liu Bing Niu Qin Chen Xueen Fang |
| author_facet | Jiale Gao Xiaodong Sun Yongxin Liu Bing Niu Qin Chen Xueen Fang |
| author_sort | Jiale Gao |
| collection | DOAJ |
| description | The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework (MOF) was synthesized and applied in loop-mediated isothermal amplification (named Cu-TCPP@LAMP), which can inhibit the non-specific amplification by absorbing and precise temperature releasing of single primer. As thus, Cu-TCPP@LAMP can achieve high sensitivity and specific amplification of the target gene. As a result, peanut and soybean allergens genes contained in food were successfully detected with a favorable detection sensitivity (5 ng/μL for peanuts and 10 ng/μL for soybeans) and reliable repeatability (The coefficient of variation was 3.38% for peanuts and 3.33% for soybeans). Moreover, the established method was utilized for detection of several commercial products, and had a high consistency with the standard method. Apart from food allergens, this novel assay can be widely used in other areas, such as pathogen detection, tumor nucleic acid detection and so on. |
| format | Article |
| id | doaj-art-703f9ff41a444e6880b8175f3a01ee70 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-703f9ff41a444e6880b8175f3a01ee702025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112517881798Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detectionJiale Gao0Xiaodong Sun1Yongxin Liu2Bing Niu3Qin Chen4Xueen Fang5Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Engineering Research Center of Organ Repair, School of Medicine, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, ChinaShanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China; Corresponding authors.Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China; Corresponding authors.The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework (MOF) was synthesized and applied in loop-mediated isothermal amplification (named Cu-TCPP@LAMP), which can inhibit the non-specific amplification by absorbing and precise temperature releasing of single primer. As thus, Cu-TCPP@LAMP can achieve high sensitivity and specific amplification of the target gene. As a result, peanut and soybean allergens genes contained in food were successfully detected with a favorable detection sensitivity (5 ng/μL for peanuts and 10 ng/μL for soybeans) and reliable repeatability (The coefficient of variation was 3.38% for peanuts and 3.33% for soybeans). Moreover, the established method was utilized for detection of several commercial products, and had a high consistency with the standard method. Apart from food allergens, this novel assay can be widely used in other areas, such as pathogen detection, tumor nucleic acid detection and so on.http://www.sciencedirect.com/science/article/pii/S2213453023000319Cu-TCPP nanosheetPlant allergenNucleic acid detectionLoop-mediated isothermal amplification |
| spellingShingle | Jiale Gao Xiaodong Sun Yongxin Liu Bing Niu Qin Chen Xueen Fang Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection Food Science and Human Wellness Cu-TCPP nanosheet Plant allergen Nucleic acid detection Loop-mediated isothermal amplification |
| title | Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection |
| title_full | Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection |
| title_fullStr | Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection |
| title_full_unstemmed | Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection |
| title_short | Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection |
| title_sort | ultrathin metal organic framework nanosheets cu tcpp based isothermal nucleic acid amplification for food allergen detection |
| topic | Cu-TCPP nanosheet Plant allergen Nucleic acid detection Loop-mediated isothermal amplification |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000319 |
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