Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair

The inhibitory effect of compound soaking solution on the quality deterioration of fish fillets during storage and its repair effect on a cell oxidative damage model were investigated. Water holding capacity, cooking loss, thawing loss, thiobarbituric acid and sensory evaluation were used to verify...

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Main Authors: Qing Shao, Zhongqiang Wang, Shumin Yi
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/14/3/442
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author Qing Shao
Zhongqiang Wang
Shumin Yi
author_facet Qing Shao
Zhongqiang Wang
Shumin Yi
author_sort Qing Shao
collection DOAJ
description The inhibitory effect of compound soaking solution on the quality deterioration of fish fillets during storage and its repair effect on a cell oxidative damage model were investigated. Water holding capacity, cooking loss, thawing loss, thiobarbituric acid and sensory evaluation were used to verify that the composite soaking solution could improve the water loss and quality deterioration of fillets during frozen storage. At 180 d, water holding capacity was increased by 4.59% in the compound soaking solution group compared with the control. Cooking loss decreased by 6.47%, and thawing loss decreased by 13.06% (<i>p</i> < 0.05). The TBA value was reduced by 50%, and the degree of lipid oxidation was lower (<i>p</i> < 0.05). The results of the microstructure analysis showed that the tissue structure of fillets treated by the compound soaking solution was more orderly. The oxidative damage model of cells was achieved by soaking in treated fish fillet digestive juice, which inhibited the increase in reactive oxygen species content, maintained the integrity of the cell structure, and increased cell viability by 32.24% (<i>p</i> < 0.05). Compound soaking solution treatment could inhibit the quality deterioration of fish fillets during storage, and the digestive solution of fish fillets could improve the oxidative stress injury of Caco-2 cells induced by H<sub>2</sub>O<sub>2</sub>.
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spelling doaj-art-702e43f627f543afa03372fa997d55e62025-08-20T02:12:30ZengMDPI AGFoods2304-81582025-01-0114344210.3390/foods14030442Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant RepairQing Shao0Zhongqiang Wang1Shumin Yi2National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, ChinaNational & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, ChinaNational & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, ChinaThe inhibitory effect of compound soaking solution on the quality deterioration of fish fillets during storage and its repair effect on a cell oxidative damage model were investigated. Water holding capacity, cooking loss, thawing loss, thiobarbituric acid and sensory evaluation were used to verify that the composite soaking solution could improve the water loss and quality deterioration of fillets during frozen storage. At 180 d, water holding capacity was increased by 4.59% in the compound soaking solution group compared with the control. Cooking loss decreased by 6.47%, and thawing loss decreased by 13.06% (<i>p</i> < 0.05). The TBA value was reduced by 50%, and the degree of lipid oxidation was lower (<i>p</i> < 0.05). The results of the microstructure analysis showed that the tissue structure of fillets treated by the compound soaking solution was more orderly. The oxidative damage model of cells was achieved by soaking in treated fish fillet digestive juice, which inhibited the increase in reactive oxygen species content, maintained the integrity of the cell structure, and increased cell viability by 32.24% (<i>p</i> < 0.05). Compound soaking solution treatment could inhibit the quality deterioration of fish fillets during storage, and the digestive solution of fish fillets could improve the oxidative stress injury of Caco-2 cells induced by H<sub>2</sub>O<sub>2</sub>.https://www.mdpi.com/2304-8158/14/3/442compound soaking solutionsea bassphysicochemical qualityCaco-2 celloxidative stress injury
spellingShingle Qing Shao
Zhongqiang Wang
Shumin Yi
Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair
Foods
compound soaking solution
sea bass
physicochemical quality
Caco-2 cell
oxidative stress injury
title Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair
title_full Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair
title_fullStr Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair
title_full_unstemmed Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair
title_short Application of Composite Soaking Solution in Fillet Storage and Caco-2 Cell Antioxidant Repair
title_sort application of composite soaking solution in fillet storage and caco 2 cell antioxidant repair
topic compound soaking solution
sea bass
physicochemical quality
Caco-2 cell
oxidative stress injury
url https://www.mdpi.com/2304-8158/14/3/442
work_keys_str_mv AT qingshao applicationofcompositesoakingsolutioninfilletstorageandcaco2cellantioxidantrepair
AT zhongqiangwang applicationofcompositesoakingsolutioninfilletstorageandcaco2cellantioxidantrepair
AT shuminyi applicationofcompositesoakingsolutioninfilletstorageandcaco2cellantioxidantrepair