Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits
Purpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitre...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/583040 |
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| author | Nakul Mandal Geoffrey P. Lewis Steven K. Fisher Steffen Heegaard Jan U. Prause Morten la Cour Henrik Vorum Bent Honoré |
| author_facet | Nakul Mandal Geoffrey P. Lewis Steven K. Fisher Steffen Heegaard Jan U. Prause Morten la Cour Henrik Vorum Bent Honoré |
| author_sort | Nakul Mandal |
| collection | DOAJ |
| description | Purpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days the eyes were enucleated and the vitreous was removed. The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry. Results. Ten protein spots were found to be at least twofold differentially expressed when comparing the vitreous samples of the sham and retinal detachment surgery groups. Protein spots that were upregulated in the vitreous following retinal detachment were identified as albumin fragments, and those downregulated were found to be peroxiredoxin 2, collagen-Iα1 fragment, and α-1-antiproteinase F. Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications. |
| format | Article |
| id | doaj-art-70236e1cb288400397b8e86b76e8850a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-70236e1cb288400397b8e86b76e8850a2025-02-03T05:45:26ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/583040583040Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in RabbitsNakul Mandal0Geoffrey P. Lewis1Steven K. Fisher2Steffen Heegaard3Jan U. Prause4Morten la Cour5Henrik Vorum6Bent Honoré7Department of Ophthalmology, Lund University, 22185 Lund, SwedenNeuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106-5060, USANeuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106-5060, USADepartment of Ophthalmology, University of Copenhagen, 2600 Glostrup, DenmarkEye Pathology Institute, University of Copenhagen, 2100 Copenhagen, DenmarkDepartment of Ophthalmology, University of Copenhagen, 2600 Glostrup, DenmarkDepartment of Ophthalmology, Aalborg University Hospital, 9000 Aalborg, DenmarkDepartment of Biomedicine, Aarhus University, 8000 Aarhus, DenmarkPurpose. The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy. Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach. Materials and Methods. Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits. Sham surgery was undertaken in five other rabbits that were used as controls. After seven days the eyes were enucleated and the vitreous was removed. The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry. Results. Ten protein spots were found to be at least twofold differentially expressed when comparing the vitreous samples of the sham and retinal detachment surgery groups. Protein spots that were upregulated in the vitreous following retinal detachment were identified as albumin fragments, and those downregulated were found to be peroxiredoxin 2, collagen-Iα1 fragment, and α-1-antiproteinase F. Conclusions. Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications.http://dx.doi.org/10.1155/2015/583040 |
| spellingShingle | Nakul Mandal Geoffrey P. Lewis Steven K. Fisher Steffen Heegaard Jan U. Prause Morten la Cour Henrik Vorum Bent Honoré Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits Journal of Ophthalmology |
| title | Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits |
| title_full | Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits |
| title_fullStr | Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits |
| title_full_unstemmed | Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits |
| title_short | Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits |
| title_sort | proteomic analysis of the vitreous following experimental retinal detachment in rabbits |
| url | http://dx.doi.org/10.1155/2015/583040 |
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