Comparison of the Effectiveness of Nigella Sativa and Vitamin E in Preventing Intra‐abdominal Adhesions
ABSTRACT Objective To compare the efficacy of locally applied Nigella sativa (NS) oil and Vitamin E (vit E) in a rat adhesion model. Animals Thirty‐six male Wistar rats (90 days old, 240 ± 37 g) Methods Animals were assigned to three groups: control (sterile saline), NS oil (10 mg/kg), and vit E (50...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-07-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70446 |
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| Summary: | ABSTRACT Objective To compare the efficacy of locally applied Nigella sativa (NS) oil and Vitamin E (vit E) in a rat adhesion model. Animals Thirty‐six male Wistar rats (90 days old, 240 ± 37 g) Methods Animals were assigned to three groups: control (sterile saline), NS oil (10 mg/kg), and vit E (50 mg/kg). After inducing intra‐abdominal adhesion, the treatments were administered intraperitoneally. On day 8, rats were euthanized, and adhesions were scored macroscopically. Levels of IL‐6, TNF‐α, PGE2, TGF‐β1, VEGF, IL‐10 and IL‐1β were measured, and histopathological evaluation and Picro‐Sirius Red staining were performed. Results Adhesion scores did not significantly differ between groups (p = 0.680). TNF‐α levels were significantly higher in the control group compared to the vit E group (p = 0.011), with no significant difference between the NS oil and vit E groups. PGE2 levels differed significantly between the NS oil and vit E groups (p = 0.023). IL‐1β, IL‐6, IL‐10, TGF‐β1 and VEGF levels did not show significant differences (p > 0.05). Histopathology revealed reduced fibrosis and inflammation in the vit E group compared to NS oil (p < 0.001). Picro‐Sirius Red staining showed improved collagen maturation in the vit E group, followed by NS oil, with the control group exhibiting less mature collagen. Conclusion Both treatments showed efficacy, but vit E was superior in reducing inflammation and fibrosis and enhancing collagen maturation. |
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| ISSN: | 2053-1095 |