LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer
Pyroptosis, a newly discovered form of programmed cell death, is involved in the occurrence, development and drug resistance of a variety of tumors and has attracted increasing attention in recent years. LINC00365 is a novel lncRNA that has...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2024-10-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024173 |
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| author | Yang Weiqing Huang Xiang Lv Weibin Jin Yuelong Zhu Yiping |
| author_facet | Yang Weiqing Huang Xiang Lv Weibin Jin Yuelong Zhu Yiping |
| author_sort | Yang Weiqing |
| collection | DOAJ |
| description | Pyroptosis, a newly discovered form of programmed cell death, is involved in the occurrence, development and drug resistance of a variety of tumors and has attracted increasing attention in recent years. LINC00365 is a novel lncRNA that has rarely been reported before. We previously reported that LINC00365 expression in colorectal cancer is closely associated with poor patient outcomes. Additionally, LINC00365 was confirmed to be positively correlated with miR-221-5p, and miR-221-5p is negatively correlated with gasdermin-D (GSDMD) in colorectal cancer tissues. Bioinformatics analysis and luciferase reporter gene experiments revealed that GSDMD is the target gene of miR-221-5p. Cell function experiments and nude mouse tumor transplantation assays confirmed that LINC00365 could regulate the expressions of pyroptosis-related proteins such as Caspase-1, Caspase-11, NLRP3 and GSDMD. RNA pulldown and RNA immunoprecipitation experiments further elucidated the mechanism by which LINC00365 regulates miR-221-5p. In the present study, we observe that LINC00365 promotes the expression of miR-221-5p by binding to the Dicer enzyme to inhibit GSDMD and plays an antipyroptotic role. Our findings suggest that LINC00365 may serve as a molecular biomarker for estimating the prognosis of patients with colorectal cancer and as a potential therapeutic target for colorectal cancer. |
| format | Article |
| id | doaj-art-700fa6c0af464d3d94240dfec754b5e9 |
| institution | DOAJ |
| issn | 1672-9145 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-700fa6c0af464d3d94240dfec754b5e92025-08-20T03:10:42ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-10-015752954110.3724/abbs.202417320d259ccLINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancerYang Weiqing0Huang Xiang1Lv Weibin2Jin Yuelong3Zhu Yiping4["School of Graduate Studies, Wannan Medical College, Wuhu 241002, China","Department of Oncology, the First Affiliated Hospital of Wannan Medical College, Wuhu 241002, China"]["School of Public Health, Wannan Medical College, Wuhu 241002, China"]["School of Graduate Studies, Wannan Medical College, Wuhu 241002, China","Department of Oncology, the First Affiliated Hospital of Wannan Medical College, Wuhu 241002, China"]["School of Public Health, Wannan Medical College, Wuhu 241002, China"]["Department of Oncology, the First Affiliated Hospital of Wannan Medical College, Wuhu 241002, China"]Pyroptosis, a newly discovered form of programmed cell death, is involved in the occurrence, development and drug resistance of a variety of tumors and has attracted increasing attention in recent years. LINC00365 is a novel lncRNA that has rarely been reported before. We previously reported that LINC00365 expression in colorectal cancer is closely associated with poor patient outcomes. Additionally, LINC00365 was confirmed to be positively correlated with miR-221-5p, and miR-221-5p is negatively correlated with gasdermin-D (GSDMD) in colorectal cancer tissues. Bioinformatics analysis and luciferase reporter gene experiments revealed that GSDMD is the target gene of miR-221-5p. Cell function experiments and nude mouse tumor transplantation assays confirmed that LINC00365 could regulate the expressions of pyroptosis-related proteins such as Caspase-1, Caspase-11, NLRP3 and GSDMD. RNA pulldown and RNA immunoprecipitation experiments further elucidated the mechanism by which LINC00365 regulates miR-221-5p. In the present study, we observe that LINC00365 promotes the expression of miR-221-5p by binding to the Dicer enzyme to inhibit GSDMD and plays an antipyroptotic role. Our findings suggest that LINC00365 may serve as a molecular biomarker for estimating the prognosis of patients with colorectal cancer and as a potential therapeutic target for colorectal cancer.https://www.sciengine.com/doi/10.3724/abbs.2024173LINC00365miR-221-5ppyroptosiscolorectal cancer |
| spellingShingle | Yang Weiqing Huang Xiang Lv Weibin Jin Yuelong Zhu Yiping LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer Acta Biochimica et Biophysica Sinica LINC00365 miR-221-5p pyroptosis colorectal cancer |
| title | LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer |
| title_full | LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer |
| title_fullStr | LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer |
| title_full_unstemmed | LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer |
| title_short | LINC00365 promotes miR-221-5p to inhibit pyroptosis via Dicer in colorectal cancer |
| title_sort | linc00365 promotes mir 221 5p to inhibit pyroptosis via dicer in colorectal cancer |
| topic | LINC00365 miR-221-5p pyroptosis colorectal cancer |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024173 |
| work_keys_str_mv | AT yangweiqing linc00365promotesmir2215ptoinhibitpyroptosisviadicerincolorectalcancer AT huangxiang linc00365promotesmir2215ptoinhibitpyroptosisviadicerincolorectalcancer AT lvweibin linc00365promotesmir2215ptoinhibitpyroptosisviadicerincolorectalcancer AT jinyuelong linc00365promotesmir2215ptoinhibitpyroptosisviadicerincolorectalcancer AT zhuyiping linc00365promotesmir2215ptoinhibitpyroptosisviadicerincolorectalcancer |