Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer
High-grade serous cancer is the most common type of ovarian cancer and is usually diagnosed at advanced stages with high mortality due to recurrence and eventual resistance to standard platinum therapy. The aim of this study was to compare two-dimensional (2D) versus tridimensional (3D) cell culture...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580824002024 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850138654891048960 |
|---|---|
| author | Naya El Mokbel Alicia A. Goyeneche Rewati Prakash Benjamin N. Forgie Farah H. Abdalbari Xing Zeng Basile Tessier-Cloutier Shuk On Annie Leung Carlos M. Telleria |
| author_facet | Naya El Mokbel Alicia A. Goyeneche Rewati Prakash Benjamin N. Forgie Farah H. Abdalbari Xing Zeng Basile Tessier-Cloutier Shuk On Annie Leung Carlos M. Telleria |
| author_sort | Naya El Mokbel |
| collection | DOAJ |
| description | High-grade serous cancer is the most common type of ovarian cancer and is usually diagnosed at advanced stages with high mortality due to recurrence and eventual resistance to standard platinum therapy. The aim of this study was to compare two-dimensional (2D) versus tridimensional (3D) cell culture as a preclinical model of response to carboplatin, paclitaxel and niraparib using PEO1, PEO4 and PEO6 cell lines, which were generated from the same patient along disease progression. Morphologically, cells formed flat adherent layers versus spheroidal structures with different compaction patterns in 2D and 3D respectively. In 2D, apoptosis was rare whereas in 3D cells formed a multilayered structure with an outer layer of live proliferating cells and an inner core of apoptotic cells. Furthermore, a differential capacity to produce ATP was observed among the cell lines in 3D but not in 2D. While response to carboplatin, paclitaxel and niraparib in both settings followed a similar trend, a lower sensitivity was observed in 3D with respect to 2D. Overall, 3D cell culture is likely more reflective of the in vivo cellular tumor behavior and more suitable of therapeutic evaluation given its added complexity absent in 2D. |
| format | Article |
| id | doaj-art-700bf3ed34844eb6b08df2d216c52d67 |
| institution | OA Journals |
| issn | 2405-5808 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-700bf3ed34844eb6b08df2d216c52d672025-08-20T02:30:32ZengElsevierBiochemistry and Biophysics Reports2405-58082024-12-014010183810.1016/j.bbrep.2024.101838Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancerNaya El Mokbel0Alicia A. Goyeneche1Rewati Prakash2Benjamin N. Forgie3Farah H. Abdalbari4Xing Zeng5Basile Tessier-Cloutier6Shuk On Annie Leung7Carlos M. Telleria8McGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, CanadaMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, CanadaMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, CanadaMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, CanadaMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, CanadaMcGill University Health Centre, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Montreal, QC, CanadaMcGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, CanadaMcGill University Health Centre, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Montreal, QC, Canada; Research Institute of the McGill University Health Centre, Cancer Research Program, Montreal, QC, Canada; Corresponding author. McGill University Health Centre, Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Montreal, QC, Canada.McGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada; Research Institute of the McGill University Health Centre, Cancer Research Program, Montreal, QC, Canada; Corresponding author. McGill University, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Montreal, QC, Canada.High-grade serous cancer is the most common type of ovarian cancer and is usually diagnosed at advanced stages with high mortality due to recurrence and eventual resistance to standard platinum therapy. The aim of this study was to compare two-dimensional (2D) versus tridimensional (3D) cell culture as a preclinical model of response to carboplatin, paclitaxel and niraparib using PEO1, PEO4 and PEO6 cell lines, which were generated from the same patient along disease progression. Morphologically, cells formed flat adherent layers versus spheroidal structures with different compaction patterns in 2D and 3D respectively. In 2D, apoptosis was rare whereas in 3D cells formed a multilayered structure with an outer layer of live proliferating cells and an inner core of apoptotic cells. Furthermore, a differential capacity to produce ATP was observed among the cell lines in 3D but not in 2D. While response to carboplatin, paclitaxel and niraparib in both settings followed a similar trend, a lower sensitivity was observed in 3D with respect to 2D. Overall, 3D cell culture is likely more reflective of the in vivo cellular tumor behavior and more suitable of therapeutic evaluation given its added complexity absent in 2D.http://www.sciencedirect.com/science/article/pii/S2405580824002024Ultra-low attachmentAnchorage-free3D culture2D cultureHigh grade serous ovarian cancerAnticancer drugs |
| spellingShingle | Naya El Mokbel Alicia A. Goyeneche Rewati Prakash Benjamin N. Forgie Farah H. Abdalbari Xing Zeng Basile Tessier-Cloutier Shuk On Annie Leung Carlos M. Telleria Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer Biochemistry and Biophysics Reports Ultra-low attachment Anchorage-free 3D culture 2D culture High grade serous ovarian cancer Anticancer drugs |
| title | Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer |
| title_full | Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer |
| title_fullStr | Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer |
| title_full_unstemmed | Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer |
| title_short | Comparison of two-dimensional and three-dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high-grade serous ovarian cancer |
| title_sort | comparison of two dimensional and three dimensional culture systems and their responses to chemotherapy in cells representing disease progression of high grade serous ovarian cancer |
| topic | Ultra-low attachment Anchorage-free 3D culture 2D culture High grade serous ovarian cancer Anticancer drugs |
| url | http://www.sciencedirect.com/science/article/pii/S2405580824002024 |
| work_keys_str_mv | AT nayaelmokbel comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT aliciaagoyeneche comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT rewatiprakash comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT benjaminnforgie comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT farahhabdalbari comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT xingzeng comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT basiletessiercloutier comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT shukonannieleung comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer AT carlosmtelleria comparisonoftwodimensionalandthreedimensionalculturesystemsandtheirresponsestochemotherapyincellsrepresentingdiseaseprogressionofhighgradeserousovariancancer |