A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis
A major health and financial burden in the chicken sector is salmonella infection. It is difficult to create an oral vaccination that can provide strong intestinal mucosal immunity in birds, particularly cross-protection against several Salmonella serotypes. As a result, the poultry industry needs a...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000264 |
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| author | Reyhaneh Sadat Moosavi-Kohnehsari Mahnaz Jafari-Sohi Tohid Piri-Gharaghie Shakiba Tolou-Shikhzadeh-Yazdi Mona Aghassizadeh-Sherbaf Romina Hosseinzadeh |
| author_facet | Reyhaneh Sadat Moosavi-Kohnehsari Mahnaz Jafari-Sohi Tohid Piri-Gharaghie Shakiba Tolou-Shikhzadeh-Yazdi Mona Aghassizadeh-Sherbaf Romina Hosseinzadeh |
| author_sort | Reyhaneh Sadat Moosavi-Kohnehsari |
| collection | DOAJ |
| description | A major health and financial burden in the chicken sector is salmonella infection. It is difficult to create an oral vaccination that can provide strong intestinal mucosal immunity in birds, particularly cross-protection against several Salmonella serotypes. As a result, the poultry industry needs a powerful oral vaccination platform that uses live bacterial vectors to prevent various Salmonella serotypes. The genetically engineered L. lactis was given orally to birds as a vaccine after a multi-epitope vector was created using a reverse vaccinology technique. After the plasmid was digested, the target group produced a 72 kDa protein called multi-epitop. Birds that received the L. lactis/pNZ8121-Multi epitope vaccination showed increased levels of interferon (IFN-γ) and NFkB1α, increased transcription rates of cytokines, and a significant presence of IgY antibodies specific to the multi epitope gene in their serum. Salmonella infection is a severe health and economic burden in the poultry industry, according to spleen sections from the L. lactis/pNZ8121-Multi epitope. Developing an oral vaccine that can provide birds robust intestinal mucosal immunity—specifically, cross-protection against many Salmonella serotypes—is challenging. The results provide a fresh method for creating new immunological candidate multi-epitome genes by using the food-grade, non-pathogenic Lactococcus lactis as a protein cell factory. This method provides a unique technique to assess the long-term sustainability, cost, safety, and usefulness of experimental pharmaceutical products. |
| format | Article |
| id | doaj-art-6ff6928f780d4601ac28b2153a054925 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-6ff6928f780d4601ac28b2153a0549252025-01-26T05:03:12ZengElsevierPoultry Science0032-57912025-02-011042104789A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactisReyhaneh Sadat Moosavi-Kohnehsari0Mahnaz Jafari-Sohi1Tohid Piri-Gharaghie2Shakiba Tolou-Shikhzadeh-Yazdi3Mona Aghassizadeh-Sherbaf4Romina Hosseinzadeh5Department of Biology, Faculty of Basic Sciences, East Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranBiotechnology Research Center, Faculty of Biological Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran; Correspondence author: Biotechnology Research Center, Faculty of Biological Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.Department of Biology, Faculty of Sciences, Islamic Azad University, Mashhad Branch, Mashhad, IranDepartment of Biology, Faculty of Basic Sciences, Islamic Azad University, East-Tehran Branch, Tehran, IranDepartment of Microbiology, Faculty of Basic Sciences, East Tehran Branch, Islamic Azad University, Tehran, IranA major health and financial burden in the chicken sector is salmonella infection. It is difficult to create an oral vaccination that can provide strong intestinal mucosal immunity in birds, particularly cross-protection against several Salmonella serotypes. As a result, the poultry industry needs a powerful oral vaccination platform that uses live bacterial vectors to prevent various Salmonella serotypes. The genetically engineered L. lactis was given orally to birds as a vaccine after a multi-epitope vector was created using a reverse vaccinology technique. After the plasmid was digested, the target group produced a 72 kDa protein called multi-epitop. Birds that received the L. lactis/pNZ8121-Multi epitope vaccination showed increased levels of interferon (IFN-γ) and NFkB1α, increased transcription rates of cytokines, and a significant presence of IgY antibodies specific to the multi epitope gene in their serum. Salmonella infection is a severe health and economic burden in the poultry industry, according to spleen sections from the L. lactis/pNZ8121-Multi epitope. Developing an oral vaccine that can provide birds robust intestinal mucosal immunity—specifically, cross-protection against many Salmonella serotypes—is challenging. The results provide a fresh method for creating new immunological candidate multi-epitome genes by using the food-grade, non-pathogenic Lactococcus lactis as a protein cell factory. This method provides a unique technique to assess the long-term sustainability, cost, safety, and usefulness of experimental pharmaceutical products.http://www.sciencedirect.com/science/article/pii/S0032579125000264Salmonella vaccineReverse vaccinologyLive bacterial vectors |
| spellingShingle | Reyhaneh Sadat Moosavi-Kohnehsari Mahnaz Jafari-Sohi Tohid Piri-Gharaghie Shakiba Tolou-Shikhzadeh-Yazdi Mona Aghassizadeh-Sherbaf Romina Hosseinzadeh A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis Poultry Science Salmonella vaccine Reverse vaccinology Live bacterial vectors |
| title | A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis |
| title_full | A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis |
| title_fullStr | A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis |
| title_full_unstemmed | A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis |
| title_short | A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis |
| title_sort | new vaccination approach for salmonellosis employing a multi epitope vaccine based on live microbial cell factory from lactococcus lactis |
| topic | Salmonella vaccine Reverse vaccinology Live bacterial vectors |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000264 |
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