White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District

Vanamei shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname (L. vannamei) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of lo...

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Main Authors: Ali Usman, Sri Andayani, Endang Yuli Herawati
Format: Article
Language:English
Published: University of Brawijaya 2018-10-01
Series:Journal of Experimental Life Science
Subjects:
Online Access:https://jels.ub.ac.id/index.php/jels/article/view/296
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author Ali Usman
Sri Andayani
Endang Yuli Herawati
author_facet Ali Usman
Sri Andayani
Endang Yuli Herawati
author_sort Ali Usman
collection DOAJ
description Vanamei shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname (L. vannamei) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of losses for farmers so it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp farms (L. vannamei) in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds site). Detection of White Spot Syndrome Virus using Nested PCR with shrimp section taken is swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs from Bangil 0 - 15%, Kraton 0% and Rejoso 0 - 15%.
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spelling doaj-art-6ff43e89bd7342a3a4059bbf8a7517442025-08-20T02:33:11ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552018-10-0183173176https://doi.org/10.21776/ub.jels.2018.008.03.07White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan DistrictAli Usman0Sri Andayani1Endang Yuli Herawati2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaVanamei shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname (L. vannamei) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of losses for farmers so it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp farms (L. vannamei) in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds site). Detection of White Spot Syndrome Virus using Nested PCR with shrimp section taken is swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs from Bangil 0 - 15%, Kraton 0% and Rejoso 0 - 15%.https://jels.ub.ac.id/index.php/jels/article/view/296pcrprevalenceshrimpvannameiwssv
spellingShingle Ali Usman
Sri Andayani
Endang Yuli Herawati
White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District
Journal of Experimental Life Science
pcr
prevalence
shrimp
vannamei
wssv
title White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District
title_full White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District
title_fullStr White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District
title_full_unstemmed White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District
title_short White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District
title_sort white spot syndrome virus wssv detection at traditional ponds of lithopenaeus vannamei in pasuruan district
topic pcr
prevalence
shrimp
vannamei
wssv
url https://jels.ub.ac.id/index.php/jels/article/view/296
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AT endangyuliherawati whitespotsyndromeviruswssvdetectionattraditionalpondsoflithopenaeusvannameiinpasuruandistrict