Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance
Quantitative nuclear magnetic resonance is a powerful tool in drug analysis because of its speed, precision, and efficiency. In present study, the application of variable temperature proton quantitative nuclear magnetic resonance (VT-1H-qNMR) for the calibration of three C-glycosylflavones including...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/4934309 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849413891584425984 |
|---|---|
| author | Jing Liu Yang Liu Zhong Dai Lan He Shuangcheng Ma |
| author_facet | Jing Liu Yang Liu Zhong Dai Lan He Shuangcheng Ma |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Quantitative nuclear magnetic resonance is a powerful tool in drug analysis because of its speed, precision, and efficiency. In present study, the application of variable temperature proton quantitative nuclear magnetic resonance (VT-1H-qNMR) for the calibration of three C-glycosylflavones including orientin, isoorientin, and schaftoside as reference substances was reported. Since there was conformational equilibrium due to the restricted rotation around the C(sp3)-C(sp2) bond in C-glycosylflavones, the conformational behaviors were investigated by VT-NMR and verified by molecular mechanics (MM) calculation. The VT-1H-qNMR method was validated including the linearity, limit of quantification, precision, and stability. The results were consistent with those obtained from mass balance approach. VT-1H-qNMR can be deployed as an effective tool in analyzing C-glycosylflavones. |
| format | Article |
| id | doaj-art-6fe0fd016bc741799d2053d3aa34fb6f |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-6fe0fd016bc741799d2053d3aa34fb6f2025-08-20T03:34:00ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/49343094934309Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic ResonanceJing Liu0Yang Liu1Zhong Dai2Lan He3Shuangcheng Ma4National Institutes for Food and Drug Control, Beijing 100050, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaQuantitative nuclear magnetic resonance is a powerful tool in drug analysis because of its speed, precision, and efficiency. In present study, the application of variable temperature proton quantitative nuclear magnetic resonance (VT-1H-qNMR) for the calibration of three C-glycosylflavones including orientin, isoorientin, and schaftoside as reference substances was reported. Since there was conformational equilibrium due to the restricted rotation around the C(sp3)-C(sp2) bond in C-glycosylflavones, the conformational behaviors were investigated by VT-NMR and verified by molecular mechanics (MM) calculation. The VT-1H-qNMR method was validated including the linearity, limit of quantification, precision, and stability. The results were consistent with those obtained from mass balance approach. VT-1H-qNMR can be deployed as an effective tool in analyzing C-glycosylflavones.http://dx.doi.org/10.1155/2017/4934309 |
| spellingShingle | Jing Liu Yang Liu Zhong Dai Lan He Shuangcheng Ma Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance Journal of Analytical Methods in Chemistry |
| title | Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance |
| title_full | Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance |
| title_fullStr | Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance |
| title_full_unstemmed | Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance |
| title_short | Structural and Quantitative Analysis of Three C-Glycosylflavones by Variable Temperature Proton Quantitative Nuclear Magnetic Resonance |
| title_sort | structural and quantitative analysis of three c glycosylflavones by variable temperature proton quantitative nuclear magnetic resonance |
| url | http://dx.doi.org/10.1155/2017/4934309 |
| work_keys_str_mv | AT jingliu structuralandquantitativeanalysisofthreecglycosylflavonesbyvariabletemperatureprotonquantitativenuclearmagneticresonance AT yangliu structuralandquantitativeanalysisofthreecglycosylflavonesbyvariabletemperatureprotonquantitativenuclearmagneticresonance AT zhongdai structuralandquantitativeanalysisofthreecglycosylflavonesbyvariabletemperatureprotonquantitativenuclearmagneticresonance AT lanhe structuralandquantitativeanalysisofthreecglycosylflavonesbyvariabletemperatureprotonquantitativenuclearmagneticresonance AT shuangchengma structuralandquantitativeanalysisofthreecglycosylflavonesbyvariabletemperatureprotonquantitativenuclearmagneticresonance |