Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum
Marine fungi have been found in all habitats and are able to adapt to their environmental niche conditions. In this study, a combination of liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) and gas chromatography coupled to mass spectrometry (GC-MS) analytical approaches w...
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| Format: | Article |
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Académie des sciences
2024-03-01
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| Series: | Comptes Rendus. Chimie |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.276/ |
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| author | Le, Van-Tuyen Bertrand, Samuel Brandolini-Bunlon, Marion Gentil, Emmanuel Robiou du Pont, Thibaut Rabesaotra, Vony Wielgosz-Collin, Gaëtane Mossion, Aurélie Grovel, Olivier |
| author_facet | Le, Van-Tuyen Bertrand, Samuel Brandolini-Bunlon, Marion Gentil, Emmanuel Robiou du Pont, Thibaut Rabesaotra, Vony Wielgosz-Collin, Gaëtane Mossion, Aurélie Grovel, Olivier |
| author_sort | Le, Van-Tuyen |
| collection | DOAJ |
| description | Marine fungi have been found in all habitats and are able to adapt to their environmental niche conditions. In this study, a combination of liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) and gas chromatography coupled to mass spectrometry (GC-MS) analytical approaches was used to analyse the metabolic changes of a marine-sourced Penicillium restrictum strain isolated from a marine shellfish area. The P. restrictum MMS417 strain was grown on seven different media including an ecological one with two different water sources (synthetic seawater and distilled water), conditions following the One-Strain-Many-Compounds (OSMAC) approach. Extracts of all media were analysed by LC-HRMS (lipids and specialised metabolites profiling) and GC-MS (fatty acids profiling). Acquired data were analysed using a multiblock strategy to highlight metabolic modification in regard to water conditions and to environmentally relevant conditions (mussel-based culture medium). This revealed that fatty acid composition of lipids was the most altered part of the explored metabolisms whether looking to the effect of water or of environmentally relevant conditions. In particular, data showed that P. restrictum MMS417 is able to produce lipids that include fatty acids usually produced by the mussel itself. This study also provides insight into the P. restrictum adaptation to marine salinity through fatty acids alteration and shows that lipid metabolism is far more altered in an OSMAC approach than the specialized metabolism. This study finally highlights the need for using environment-mimicking culture conditions to reveal the metabolic potentialities of marine microbes. |
| format | Article |
| id | doaj-art-6fda572bfd7540649bce7dc179ffafec |
| institution | Kabale University |
| issn | 1878-1543 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus. Chimie |
| spelling | doaj-art-6fda572bfd7540649bce7dc179ffafec2025-02-07T13:38:15ZengAcadémie des sciencesComptes Rendus. Chimie1878-15432024-03-0126S2496610.5802/crchim.27610.5802/crchim.276Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictumLe, Van-Tuyen0Bertrand, Samuel1https://orcid.org/0000-0002-5934-7012Brandolini-Bunlon, Marion2https://orcid.org/0000-0003-0131-8216Gentil, Emmanuel3https://orcid.org/0000-0001-8108-3693Robiou du Pont, Thibaut4https://orcid.org/0009-0005-7121-7711Rabesaotra, Vony5https://orcid.org/0000-0002-2670-3754Wielgosz-Collin, Gaëtane6https://orcid.org/0000-0001-7355-8193Mossion, Aurélie7https://orcid.org/0000-0002-8391-8938Grovel, Olivier8https://orcid.org/0000-0003-3165-4771Biotechnology Division, Vietnam-Korea Institute of Science and Technology, Hoa Lac High-tech Park, Thach That province, Ha Noi, Vietnam; Nantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, France; Phu Tho College of Pharmacy, 290000 Phu Tho, VietnamNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceUniversité Clermont Auvergne, INRAE, UNH, Plateforme d’Exploration du Métabolisme, MetaboHUB Clermont, 63000 Clermont-Ferrand, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMER, UR 2160, F-44000 Nantes, FranceMarine fungi have been found in all habitats and are able to adapt to their environmental niche conditions. In this study, a combination of liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) and gas chromatography coupled to mass spectrometry (GC-MS) analytical approaches was used to analyse the metabolic changes of a marine-sourced Penicillium restrictum strain isolated from a marine shellfish area. The P. restrictum MMS417 strain was grown on seven different media including an ecological one with two different water sources (synthetic seawater and distilled water), conditions following the One-Strain-Many-Compounds (OSMAC) approach. Extracts of all media were analysed by LC-HRMS (lipids and specialised metabolites profiling) and GC-MS (fatty acids profiling). Acquired data were analysed using a multiblock strategy to highlight metabolic modification in regard to water conditions and to environmentally relevant conditions (mussel-based culture medium). This revealed that fatty acid composition of lipids was the most altered part of the explored metabolisms whether looking to the effect of water or of environmentally relevant conditions. In particular, data showed that P. restrictum MMS417 is able to produce lipids that include fatty acids usually produced by the mussel itself. This study also provides insight into the P. restrictum adaptation to marine salinity through fatty acids alteration and shows that lipid metabolism is far more altered in an OSMAC approach than the specialized metabolism. This study finally highlights the need for using environment-mimicking culture conditions to reveal the metabolic potentialities of marine microbes.https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.276/Marine-derived fungiLipidsOne-strain-many-compounds (OSMAC)Ecological mediaMetabolomeMultiblock |
| spellingShingle | Le, Van-Tuyen Bertrand, Samuel Brandolini-Bunlon, Marion Gentil, Emmanuel Robiou du Pont, Thibaut Rabesaotra, Vony Wielgosz-Collin, Gaëtane Mossion, Aurélie Grovel, Olivier Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum Comptes Rendus. Chimie Marine-derived fungi Lipids One-strain-many-compounds (OSMAC) Ecological media Metabolome Multiblock |
| title | Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum |
| title_full | Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum |
| title_fullStr | Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum |
| title_full_unstemmed | Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum |
| title_short | Global metabolome changes induced by environmentally relevant conditions in a marine-sourced Penicillium restrictum |
| title_sort | global metabolome changes induced by environmentally relevant conditions in a marine sourced penicillium restrictum |
| topic | Marine-derived fungi Lipids One-strain-many-compounds (OSMAC) Ecological media Metabolome Multiblock |
| url | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.276/ |
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