Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve

The quality of fermented milk can be improved by the exopolysaccharides (EPSs) produced by probiotics. Bifidobacterium breve H4-2 and H9-3 with different EPS production were used as co-fermented probiotics to ferment goat milk in the present study. The physicochemical properties of fermented goat mi...

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Main Authors: Mengmeng Niu, Qiwen Jin, Huanxin Guo, Xiangchen Meng
Format: Article
Language:English
Published: Tsinghua University Press 2025-11-01
Series:Food Science of Animal Products
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Online Access:https://www.sciopen.com/article/10.26599/FSAP.2025.9240121
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author Mengmeng Niu
Qiwen Jin
Huanxin Guo
Xiangchen Meng
author_facet Mengmeng Niu
Qiwen Jin
Huanxin Guo
Xiangchen Meng
author_sort Mengmeng Niu
collection DOAJ
description The quality of fermented milk can be improved by the exopolysaccharides (EPSs) produced by probiotics. Bifidobacterium breve H4-2 and H9-3 with different EPS production were used as co-fermented probiotics to ferment goat milk in the present study. The physicochemical properties of fermented goat milk and the interaction between goat milk casein and CEPS (EPSs separated from starter culture and B. breve H4-2 fermented goat milk) were investigated. Results indicated that both B. breve H4-2 and H9-3 have similar gene clusters for EPS synthesis, but B. breve H4-2 exhibits a higher ability in EPS productions. Compared to the starter culture group, combining B. breve H4-2 or H9-3 with a starter culture significantly increased the levels of EPSs ((724.16 ± 1.54) and (607.15 ± 4.55) mg/L) and lactic acid ((14.62 ± 0.02) and (16.87 ± 0.01) mg/mL) at the late storage period. Co-fermentation also enhanced the water holding capacity, firmness, consistency, cohesion, and viscosity index, reduced dehydration in fermented goat milk. Moreover, the interaction study revealed that CEPS reduced the particle size and increased the ζ-potential absolute value of the CEPS and casein mixture system. Additionally, CEPS effectively mitigated casein aggregation, thereby enhancing the stability of fermented goat milk. These findings present a novel perspective for the development of a natural starter.
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publisher Tsinghua University Press
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spelling doaj-art-6fd0586f3f6a4f99b3cfbf4cfb5d016a2025-08-20T03:32:45ZengTsinghua University PressFood Science of Animal Products2958-41242958-37802025-11-0133924012110.26599/FSAP.2025.9240121Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breveMengmeng Niu0Qiwen Jin1Huanxin Guo2Xiangchen Meng3Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaThe quality of fermented milk can be improved by the exopolysaccharides (EPSs) produced by probiotics. Bifidobacterium breve H4-2 and H9-3 with different EPS production were used as co-fermented probiotics to ferment goat milk in the present study. The physicochemical properties of fermented goat milk and the interaction between goat milk casein and CEPS (EPSs separated from starter culture and B. breve H4-2 fermented goat milk) were investigated. Results indicated that both B. breve H4-2 and H9-3 have similar gene clusters for EPS synthesis, but B. breve H4-2 exhibits a higher ability in EPS productions. Compared to the starter culture group, combining B. breve H4-2 or H9-3 with a starter culture significantly increased the levels of EPSs ((724.16 ± 1.54) and (607.15 ± 4.55) mg/L) and lactic acid ((14.62 ± 0.02) and (16.87 ± 0.01) mg/mL) at the late storage period. Co-fermentation also enhanced the water holding capacity, firmness, consistency, cohesion, and viscosity index, reduced dehydration in fermented goat milk. Moreover, the interaction study revealed that CEPS reduced the particle size and increased the ζ-potential absolute value of the CEPS and casein mixture system. Additionally, CEPS effectively mitigated casein aggregation, thereby enhancing the stability of fermented goat milk. These findings present a novel perspective for the development of a natural starter.https://www.sciopen.com/article/10.26599/FSAP.2025.9240121bifidobacterium breveexopolysaccharidefermented goat milkcasein
spellingShingle Mengmeng Niu
Qiwen Jin
Huanxin Guo
Xiangchen Meng
Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve
Food Science of Animal Products
bifidobacterium breve
exopolysaccharide
fermented goat milk
casein
title Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve
title_full Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve
title_fullStr Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve
title_full_unstemmed Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve
title_short Study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide-producing Bifidobacterium breve
title_sort study on improving the quality of fermented goat milk and its mechanism by exopolysaccharide producing bifidobacterium breve
topic bifidobacterium breve
exopolysaccharide
fermented goat milk
casein
url https://www.sciopen.com/article/10.26599/FSAP.2025.9240121
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