Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress
The study aims to investigate the immunological role of chlorogenic acid (CA) on the intestinal health of broiler under chronic heat stress. 240 broilers were randomly divided into 4 groups: control group with a feeding temperature of (22 ± 2) °C, and the other three groups with a feeding temperatur...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125005425 |
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| author | Aftab Shaukat Sana Hanif Rizwan Shukat Muhammad Tahir Aleem Irfan Shaukat Mikhlid H. Almutairi Bader O. Almutairi Mubashar Hassan Shahid Ali Rajput Shu-cheng Huang Kasim Sakran Abass Ren-Wei Su |
| author_facet | Aftab Shaukat Sana Hanif Rizwan Shukat Muhammad Tahir Aleem Irfan Shaukat Mikhlid H. Almutairi Bader O. Almutairi Mubashar Hassan Shahid Ali Rajput Shu-cheng Huang Kasim Sakran Abass Ren-Wei Su |
| author_sort | Aftab Shaukat |
| collection | DOAJ |
| description | The study aims to investigate the immunological role of chlorogenic acid (CA) on the intestinal health of broiler under chronic heat stress. 240 broilers were randomly divided into 4 groups: control group with a feeding temperature of (22 ± 2) °C, and the other three groups with a feeding temperature of (30 ± 2) °C. The chronic heat stress group (HS group) was fed with the basic diet daily, while the high-dose CA groups (HCA group) and low-dose (LCA group) were supplemented with CA at 600 mg/kg and 300 mg/kg, respectively. The health performance, intestinal antioxidant indicators, inflammatory factors, heat shock proteins, and barrier integrity-related protein expression were tested. The results showed that 600 mg/kg CA in the basic diet was promoted the morphological and cellular performance of intestinal epithelial cells of heat-stressed broilers and significantly increased the ADG (P < 0.05). HCA significantly improved the activity of SOD and CAT in the intestinal tract of heat-stressed broilers (P < 0.05), and significantly reduced the content of peroxides MDA (P < 0.05). HCA significantly inhibited the concentration of IL-1β, IL-6, and TNF-α (P < 0.05), significantly increased the content of IL-10 (P < 0.05). Further, HCA significantly downregulated the proteins and genes expression of HSP27, HSP70, and HSP90 (P < 0.05), and significantly upregulated the proteins and genes expression of Claudin1, Occludin, and ZO-1 (P < 0.05), thereby promoting intestinal barrier integrity. These findings highlight the potential of CA as a functional additive to mitigate the adverse effects of heat stress in poultry production. |
| format | Article |
| id | doaj-art-6fc50bd962bc4a3ab8be8d9435f1e812 |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-6fc50bd962bc4a3ab8be8d9435f1e8122025-08-20T03:08:22ZengElsevierPoultry Science0032-57912025-08-01104810530010.1016/j.psj.2025.105300Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stressAftab Shaukat0Sana Hanif1Rizwan Shukat2Muhammad Tahir Aleem3Irfan Shaukat4Mikhlid H. Almutairi5Bader O. Almutairi6Mubashar Hassan7Shahid Ali Rajput8Shu-cheng Huang9Kasim Sakran Abass10Ren-Wei Su11College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaHubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Wuhan University of Technology, Wuhan 430070, ChinaFaculty of Food, Nutrition & Home Sciences, University of Agriculture, Faisalabad 38000, PakistanDepartment of Pharmacology, Shantou University Medical College, Shantou 515041, ChinaDepartment of Biochemistry, University of Narowal, Narowal 51600, PakistanZoology Department, College of Science, King Saud University, P.O. Box: 2455, 11451 Riyadh, Saudi ArabiaZoology Department, College of Science, King Saud University, P.O. Box: 2455, 11451 Riyadh, Saudi ArabiaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaFaculty of Veterinary and Animal Sciences, MNS-University of Agriculture, Multan 66000, PakistanCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, ChinaDepartment of Physiology, Biochemistry, and Pharmacology; College of Veterinary Medicine; University of Kirkuk; Kirkuk 36001, IraqCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaThe study aims to investigate the immunological role of chlorogenic acid (CA) on the intestinal health of broiler under chronic heat stress. 240 broilers were randomly divided into 4 groups: control group with a feeding temperature of (22 ± 2) °C, and the other three groups with a feeding temperature of (30 ± 2) °C. The chronic heat stress group (HS group) was fed with the basic diet daily, while the high-dose CA groups (HCA group) and low-dose (LCA group) were supplemented with CA at 600 mg/kg and 300 mg/kg, respectively. The health performance, intestinal antioxidant indicators, inflammatory factors, heat shock proteins, and barrier integrity-related protein expression were tested. The results showed that 600 mg/kg CA in the basic diet was promoted the morphological and cellular performance of intestinal epithelial cells of heat-stressed broilers and significantly increased the ADG (P < 0.05). HCA significantly improved the activity of SOD and CAT in the intestinal tract of heat-stressed broilers (P < 0.05), and significantly reduced the content of peroxides MDA (P < 0.05). HCA significantly inhibited the concentration of IL-1β, IL-6, and TNF-α (P < 0.05), significantly increased the content of IL-10 (P < 0.05). Further, HCA significantly downregulated the proteins and genes expression of HSP27, HSP70, and HSP90 (P < 0.05), and significantly upregulated the proteins and genes expression of Claudin1, Occludin, and ZO-1 (P < 0.05), thereby promoting intestinal barrier integrity. These findings highlight the potential of CA as a functional additive to mitigate the adverse effects of heat stress in poultry production.http://www.sciencedirect.com/science/article/pii/S0032579125005425BroilerChronic heat stressChlorogenic acidIntestinal barrierInflammatory factorsHeat shock protein |
| spellingShingle | Aftab Shaukat Sana Hanif Rizwan Shukat Muhammad Tahir Aleem Irfan Shaukat Mikhlid H. Almutairi Bader O. Almutairi Mubashar Hassan Shahid Ali Rajput Shu-cheng Huang Kasim Sakran Abass Ren-Wei Su Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress Poultry Science Broiler Chronic heat stress Chlorogenic acid Intestinal barrier Inflammatory factors Heat shock protein |
| title | Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress |
| title_full | Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress |
| title_fullStr | Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress |
| title_full_unstemmed | Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress |
| title_short | Immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress |
| title_sort | immunological role of chlorogenic acid in broiler intestinal health under chronic heat stress |
| topic | Broiler Chronic heat stress Chlorogenic acid Intestinal barrier Inflammatory factors Heat shock protein |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125005425 |
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