Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis
Vital pulp therapy is critically important for the long-term preservation of teeth with pulpitis. However, the anti-inflammatory effects of pulp-capping materials used in clinics remain unsatisfactory. Pulpitis is tightly connected with oxidative stresses; therefore, scavenging excessive reactive ox...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000407 |
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| author | Lixin Zhang Xianling Ning Jingyi Cui Yanqiang Zhao Fei Xie Ningxin Zhu Dan Wang Man Qin Zhiqiang Lin Yuanyuan Wang |
| author_facet | Lixin Zhang Xianling Ning Jingyi Cui Yanqiang Zhao Fei Xie Ningxin Zhu Dan Wang Man Qin Zhiqiang Lin Yuanyuan Wang |
| author_sort | Lixin Zhang |
| collection | DOAJ |
| description | Vital pulp therapy is critically important for the long-term preservation of teeth with pulpitis. However, the anti-inflammatory effects of pulp-capping materials used in clinics remain unsatisfactory. Pulpitis is tightly connected with oxidative stresses; therefore, scavenging excessive reactive oxygen species (ROS) is a prospective treatment. Here a thermosensitive and injectable hydrogel that forms in situ was developed. By using poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide)-based triblock copolymer (PPP) as a scaffold to carry Prussian blue nanoparticles (PBNPs), with effective ROS scavenging capability via multiple enzyme-like activities, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)activities. The developed PBNPs@PPP demonstrated anti-oxidative stress and anti-inflammation capabilities in vitro. As a direct pulp capping material, PBNPs@PPP suppressed inflammation progression in pulpitis in rats while also reducing cell necrosis and inflammatory cell infiltration in pulp tissue. RNA sequencing analyses showed that the functions of PBNPs@PPP were highly involved in anti-inflammatory pathways. Through experimental verification, it was found that PBNPs@PPP suppressed inflammation progression in pulpitis by inhibiting the NF-κB/NLRP3 pathway, which reduced the transmission of downstream inflammation pathways. Overall, use of this PBNPs@PPP hydrogel has potential to be a new, safe and efficient strategy for vital pulp therapy to treat pulpitis. |
| format | Article |
| id | doaj-art-6fc05a3184354e689b4774a83674d1af |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-6fc05a3184354e689b4774a83674d1af2025-01-30T05:14:49ZengElsevierMaterials Today Bio2590-00642025-04-0131101482Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitisLixin Zhang0Xianling Ning1Jingyi Cui2Yanqiang Zhao3Fei Xie4Ningxin Zhu5Dan Wang6Man Qin7Zhiqiang Lin8Yuanyuan Wang9Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaInstitute of Systems Biomedicine, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing, 100191, PR ChinaDepartment of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaDepartment of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaDepartment of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaDepartment of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaDepartment of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaDepartment of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR ChinaInstitute of Systems Biomedicine, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing, 100191, PR China; Corresponding author.Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, Beijing, 100081, PR China; Corresponding author.Vital pulp therapy is critically important for the long-term preservation of teeth with pulpitis. However, the anti-inflammatory effects of pulp-capping materials used in clinics remain unsatisfactory. Pulpitis is tightly connected with oxidative stresses; therefore, scavenging excessive reactive oxygen species (ROS) is a prospective treatment. Here a thermosensitive and injectable hydrogel that forms in situ was developed. By using poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide)-based triblock copolymer (PPP) as a scaffold to carry Prussian blue nanoparticles (PBNPs), with effective ROS scavenging capability via multiple enzyme-like activities, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)activities. The developed PBNPs@PPP demonstrated anti-oxidative stress and anti-inflammation capabilities in vitro. As a direct pulp capping material, PBNPs@PPP suppressed inflammation progression in pulpitis in rats while also reducing cell necrosis and inflammatory cell infiltration in pulp tissue. RNA sequencing analyses showed that the functions of PBNPs@PPP were highly involved in anti-inflammatory pathways. Through experimental verification, it was found that PBNPs@PPP suppressed inflammation progression in pulpitis by inhibiting the NF-κB/NLRP3 pathway, which reduced the transmission of downstream inflammation pathways. Overall, use of this PBNPs@PPP hydrogel has potential to be a new, safe and efficient strategy for vital pulp therapy to treat pulpitis.http://www.sciencedirect.com/science/article/pii/S2590006425000407Prussian blue nanoparticlesThermosensitive hydrogelVital pulp therapyPulpitis |
| spellingShingle | Lixin Zhang Xianling Ning Jingyi Cui Yanqiang Zhao Fei Xie Ningxin Zhu Dan Wang Man Qin Zhiqiang Lin Yuanyuan Wang Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis Materials Today Bio Prussian blue nanoparticles Thermosensitive hydrogel Vital pulp therapy Pulpitis |
| title | Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis |
| title_full | Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis |
| title_fullStr | Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis |
| title_full_unstemmed | Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis |
| title_short | Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis |
| title_sort | thermosensitive injectable in situ forming hydrogel incorporating anti oxidative stress and anti inflammatory nanoparticles for vital pulp therapy of pulpitis |
| topic | Prussian blue nanoparticles Thermosensitive hydrogel Vital pulp therapy Pulpitis |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000407 |
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