Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases

Pests and pathogens are a primary threat to honey bee (Apis mellifera) colonies worldwide. Selective breeding for honey bees resistant to these stressors represents a promising approach for mitigating their impacts on honey bee health. UBeeO is a novel hygiene-eliciting selection tool that has been...

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Main Authors: Samantha A. Alger, P. Alexander Burnham, M. Sydney Miller, Esmaeil Amiri, Corinne Jordan, Kaira Wagoner
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Bee Science
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Online Access:https://www.frontiersin.org/articles/10.3389/frbee.2025.1509871/full
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author Samantha A. Alger
P. Alexander Burnham
M. Sydney Miller
Esmaeil Amiri
Esmaeil Amiri
Corinne Jordan
Kaira Wagoner
author_facet Samantha A. Alger
P. Alexander Burnham
M. Sydney Miller
Esmaeil Amiri
Esmaeil Amiri
Corinne Jordan
Kaira Wagoner
author_sort Samantha A. Alger
collection DOAJ
description Pests and pathogens are a primary threat to honey bee (Apis mellifera) colonies worldwide. Selective breeding for honey bees resistant to these stressors represents a promising approach for mitigating their impacts on honey bee health. UBeeO is a novel hygiene-eliciting selection tool that has been used to identify honey bee colonies that are resistant to the parasitic mite Varroa destructor, and that are more likely to survive winter without beekeeper intervention. Here, we used three separate case studies to evaluate the effectiveness of the UBeeO assay in identifying colonies resist to disease. In three distinct geographic regions, we measured UBeeO scores along with the prevalence and load of key fungal and viral honey bee pathogens. We show that UBeeO can be used to identify colonies resistant to several other diseases, including the two fungal pathogens chalkbrood (Ascosphaera apis) and Vairimorpha spp. (previously Nosema), and multiple viruses, all critically important to honey bee health and survival. Furthermore, we identify potential UBeeO resistance thresholds for each pathogen, demonstrating an inverse relationship between pathogen virulence and the minimum UBeeO score associated with resistance to that pathogen. These findings suggest that UBeeO-guided selection strategies have the potential to significantly improve honey bee breeding programs by facilitating identification of resilient and pathogen-resistant colonies. The broad geographic range of our study sites underscores the robustness and applicability of UBeeO across varying environmental contexts. Since honey bees provide essential pollination services in both natural and agricultural ecosystems, this work has major implications for environmental health, crop productivity, and food security on a global scale.
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spelling doaj-art-6fb9eaf64fe248329f46901e4fcb42d42025-08-20T03:10:01ZengFrontiers Media S.A.Frontiers in Bee Science2813-59112025-04-01310.3389/frbee.2025.15098711509871Unhealthy brood odor scores predict pathogen loads of several important honey bee diseasesSamantha A. Alger0P. Alexander Burnham1M. Sydney Miller2Esmaeil Amiri3Esmaeil Amiri4Corinne Jordan5Kaira Wagoner6Agriculture, Landscape, and Environment Department, University of Vermont, Burlington, VT, United StatesBiology Department, University of Vermont, Burlington, VT, United StatesAgriculture, Landscape, and Environment Department, University of Vermont, Burlington, VT, United StatesDelta Research and Extension Center, Mississippi State University, Stoneville, MS, United StatesBiology Department, University of North Carolina, Greensboro, NC, United StatesThe Bee Lady Apiaries, Carbrook, QLD, AustraliaBiology Department, University of North Carolina, Greensboro, NC, United StatesPests and pathogens are a primary threat to honey bee (Apis mellifera) colonies worldwide. Selective breeding for honey bees resistant to these stressors represents a promising approach for mitigating their impacts on honey bee health. UBeeO is a novel hygiene-eliciting selection tool that has been used to identify honey bee colonies that are resistant to the parasitic mite Varroa destructor, and that are more likely to survive winter without beekeeper intervention. Here, we used three separate case studies to evaluate the effectiveness of the UBeeO assay in identifying colonies resist to disease. In three distinct geographic regions, we measured UBeeO scores along with the prevalence and load of key fungal and viral honey bee pathogens. We show that UBeeO can be used to identify colonies resistant to several other diseases, including the two fungal pathogens chalkbrood (Ascosphaera apis) and Vairimorpha spp. (previously Nosema), and multiple viruses, all critically important to honey bee health and survival. Furthermore, we identify potential UBeeO resistance thresholds for each pathogen, demonstrating an inverse relationship between pathogen virulence and the minimum UBeeO score associated with resistance to that pathogen. These findings suggest that UBeeO-guided selection strategies have the potential to significantly improve honey bee breeding programs by facilitating identification of resilient and pathogen-resistant colonies. The broad geographic range of our study sites underscores the robustness and applicability of UBeeO across varying environmental contexts. Since honey bees provide essential pollination services in both natural and agricultural ecosystems, this work has major implications for environmental health, crop productivity, and food security on a global scale.https://www.frontiersin.org/articles/10.3389/frbee.2025.1509871/fullhoney beehygienepathogenRNA virusVairimorphaNosema
spellingShingle Samantha A. Alger
P. Alexander Burnham
M. Sydney Miller
Esmaeil Amiri
Esmaeil Amiri
Corinne Jordan
Kaira Wagoner
Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
Frontiers in Bee Science
honey bee
hygiene
pathogen
RNA virus
Vairimorpha
Nosema
title Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
title_full Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
title_fullStr Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
title_full_unstemmed Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
title_short Unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
title_sort unhealthy brood odor scores predict pathogen loads of several important honey bee diseases
topic honey bee
hygiene
pathogen
RNA virus
Vairimorpha
Nosema
url https://www.frontiersin.org/articles/10.3389/frbee.2025.1509871/full
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