The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model
Objective. Semaphorin3E (Sema3E) mediates reorganization of the actin cytoskeleton, and plays an important role in ensuring the specificity of synapse formation and angiogenesis. However, the role of Sema3E in allergic asthma (AS) and eosinophilic bronchitis (EB) is still elusive. This study aimed t...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2023/6459234 |
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| author | Liyan Chen Xiaohui Yuan Yaowei He Zichuan Fan Ya Guan Qiuying Li Yaying Chen Lianglan Bao Yidan Huang Kefang Lai |
| author_facet | Liyan Chen Xiaohui Yuan Yaowei He Zichuan Fan Ya Guan Qiuying Li Yaying Chen Lianglan Bao Yidan Huang Kefang Lai |
| author_sort | Liyan Chen |
| collection | DOAJ |
| description | Objective. Semaphorin3E (Sema3E) mediates reorganization of the actin cytoskeleton, and plays an important role in ensuring the specificity of synapse formation and angiogenesis. However, the role of Sema3E in allergic asthma (AS) and eosinophilic bronchitis (EB) is still elusive. This study aimed to investigate the relationship between Sema3E in vagal ganglion and lung tissue, airway reactivity, and eosinophilic inflammation. Methods. The frequency of coughs and airway reactivity as well as the airway inflammation were observed in ovalbumin- (OVA-) induced AS and EB mouse models. The expression of Sema3E was examined in the vagal ganglion and lung tissues by immunofluorescence staining and western blotting analyses. In the Sema3E treatment protocol, exogenous Sema3E was administrated intranasally before challenge in AS model to study the effect of Sema3E on airway hyperresponsiveness, airway inflammation, mucus production, and collagen deposition. Results. The similar higher frequency of coughs and airway eosinophilic inflammation could be seen in AS and EB groups compared with nasal saline (NS) and dexamethasone (DXM) groups. The absence of the airway hyperresponsiveness was observed in EB and DXM group, while AS group showed increase in airway reactivity to methacholine. The expression of Sema3E in vagal ganglion and lung tissue was remarkably decreased in AS and DXM group compared with EB group. Sema3E-treated asthma mice displayed ameliorated airway hyperresponsiveness, mucus production, and collagen deposition. Conclusion. Sema3E in lungs and vagal ganglia is related to eosinophilic inflammation and has a protective effect on OVA-induced AHR in asthma. |
| format | Article |
| id | doaj-art-6fb8adabfc20418fa010424812c25bda |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6fb8adabfc20418fa010424812c25bda2025-08-20T03:34:04ZengWileyJournal of Immunology Research2314-71562023-01-01202310.1155/2023/6459234The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma ModelLiyan Chen0Xiaohui Yuan1Yaowei He2Zichuan Fan3Ya Guan4Qiuying Li5Yaying Chen6Lianglan Bao7Yidan Huang8Kefang Lai9The First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityThe First Affiliated Hospital of Guangzhou Medical UniversityObjective. Semaphorin3E (Sema3E) mediates reorganization of the actin cytoskeleton, and plays an important role in ensuring the specificity of synapse formation and angiogenesis. However, the role of Sema3E in allergic asthma (AS) and eosinophilic bronchitis (EB) is still elusive. This study aimed to investigate the relationship between Sema3E in vagal ganglion and lung tissue, airway reactivity, and eosinophilic inflammation. Methods. The frequency of coughs and airway reactivity as well as the airway inflammation were observed in ovalbumin- (OVA-) induced AS and EB mouse models. The expression of Sema3E was examined in the vagal ganglion and lung tissues by immunofluorescence staining and western blotting analyses. In the Sema3E treatment protocol, exogenous Sema3E was administrated intranasally before challenge in AS model to study the effect of Sema3E on airway hyperresponsiveness, airway inflammation, mucus production, and collagen deposition. Results. The similar higher frequency of coughs and airway eosinophilic inflammation could be seen in AS and EB groups compared with nasal saline (NS) and dexamethasone (DXM) groups. The absence of the airway hyperresponsiveness was observed in EB and DXM group, while AS group showed increase in airway reactivity to methacholine. The expression of Sema3E in vagal ganglion and lung tissue was remarkably decreased in AS and DXM group compared with EB group. Sema3E-treated asthma mice displayed ameliorated airway hyperresponsiveness, mucus production, and collagen deposition. Conclusion. Sema3E in lungs and vagal ganglia is related to eosinophilic inflammation and has a protective effect on OVA-induced AHR in asthma.http://dx.doi.org/10.1155/2023/6459234 |
| spellingShingle | Liyan Chen Xiaohui Yuan Yaowei He Zichuan Fan Ya Guan Qiuying Li Yaying Chen Lianglan Bao Yidan Huang Kefang Lai The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model Journal of Immunology Research |
| title | The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model |
| title_full | The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model |
| title_fullStr | The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model |
| title_full_unstemmed | The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model |
| title_short | The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model |
| title_sort | expression of semaphorin3e in vagal ganglion and lung tissue is related to airway hyperresponsiveness in murine asthma model |
| url | http://dx.doi.org/10.1155/2023/6459234 |
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