Dynamics of the In vitro Growing of Mycobacterium bovis from the Lungs of Vaccinated and Infected Mice
Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of an...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2025-04-01
|
| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijmy.ijmy_32_25 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background:
Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment.
Methods:
CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test.
Results:
On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing.
Conclusion:
These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials. |
|---|---|
| ISSN: | 2212-5531 2212-554X |