Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China
Feline astroviruses (FeAstVs) have been increasingly detected in cats in recent years, yet their isolation and pathogenicity remain poorly characterized. In this study, we screened 86 feline diarrheal samples and identified FeAstV in 9.3% (8/86) of the cases, most of which were co-infected with feli...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1614686/full |
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| author | Wenjie Wang Ya Zhang Ya Zhang Xiang Wang Xiang Wang Xu Zhu Li Gong Zheng Jing Ting Meng Jiayu Shen Yeping Tan Yeping Tan Tong Qin Zhimin Li Zhimin Li Zhenwei Bi Zhenwei Bi |
| author_facet | Wenjie Wang Ya Zhang Ya Zhang Xiang Wang Xiang Wang Xu Zhu Li Gong Zheng Jing Ting Meng Jiayu Shen Yeping Tan Yeping Tan Tong Qin Zhimin Li Zhimin Li Zhenwei Bi Zhenwei Bi |
| author_sort | Wenjie Wang |
| collection | DOAJ |
| description | Feline astroviruses (FeAstVs) have been increasingly detected in cats in recent years, yet their isolation and pathogenicity remain poorly characterized. In this study, we screened 86 feline diarrheal samples and identified FeAstV in 9.3% (8/86) of the cases, most of which were co-infected with feline parvovirus (FPV). A FeAstV strain (22SDWH1003-16) was successfully isolated in F81 cells from a single FeAstV positive sample, inducing cytopathic effects (CPEs) over 15 passages. The phylogenetic tree of ORF2 classified the isolate within Mamastrovirus 2 group 1, the most common in the inter-specific transmission within cats. Experimental inoculation of four cats revealed seroconversion in all animals, transient fecal shedding in 3/4 cats, and self-limiting diarrhea in one individual. Co-infection experiments demonstrated enhanced FeAstV replication in the presence of FPV. Our findings provide the direct evidence of FeAstV-induced diarrhea in cats and highlight the role of viral co-infections in disease severity. |
| format | Article |
| id | doaj-art-6f9442f44d414743ae57ef82dc536abb |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-6f9442f44d414743ae57ef82dc536abb2025-08-20T03:08:20ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-07-011210.3389/fvets.2025.16146861614686Isolation and preliminary pathogenicity of a recent feline astrovirus strain in ChinaWenjie Wang0Ya Zhang1Ya Zhang2Xiang Wang3Xiang Wang4Xu Zhu5Li Gong6Zheng Jing7Ting Meng8Jiayu Shen9Yeping Tan10Yeping Tan11Tong Qin12Zhimin Li13Zhimin Li14Zhenwei Bi15Zhenwei Bi16School of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, National Center for Engineering Research of Veterinary Bio-products, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, National Center for Engineering Research of Veterinary Bio-products, Nanjing, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaShanghai GlinX Biotechnology Company Limited, Shanghai, ChinaShanghai GlinX Biotechnology Company Limited, Shanghai, ChinaSchool of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, ChinaSchool of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, ChinaSchool of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, National Center for Engineering Research of Veterinary Bio-products, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences (CAAS), Beijing, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, National Center for Engineering Research of Veterinary Bio-products, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, National Center for Engineering Research of Veterinary Bio-products, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaFeline astroviruses (FeAstVs) have been increasingly detected in cats in recent years, yet their isolation and pathogenicity remain poorly characterized. In this study, we screened 86 feline diarrheal samples and identified FeAstV in 9.3% (8/86) of the cases, most of which were co-infected with feline parvovirus (FPV). A FeAstV strain (22SDWH1003-16) was successfully isolated in F81 cells from a single FeAstV positive sample, inducing cytopathic effects (CPEs) over 15 passages. The phylogenetic tree of ORF2 classified the isolate within Mamastrovirus 2 group 1, the most common in the inter-specific transmission within cats. Experimental inoculation of four cats revealed seroconversion in all animals, transient fecal shedding in 3/4 cats, and self-limiting diarrhea in one individual. Co-infection experiments demonstrated enhanced FeAstV replication in the presence of FPV. Our findings provide the direct evidence of FeAstV-induced diarrhea in cats and highlight the role of viral co-infections in disease severity.https://www.frontiersin.org/articles/10.3389/fvets.2025.1614686/fullFeAstVisolationpathogenicitydiarrheaco-infection |
| spellingShingle | Wenjie Wang Ya Zhang Ya Zhang Xiang Wang Xiang Wang Xu Zhu Li Gong Zheng Jing Ting Meng Jiayu Shen Yeping Tan Yeping Tan Tong Qin Zhimin Li Zhimin Li Zhenwei Bi Zhenwei Bi Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China Frontiers in Veterinary Science FeAstV isolation pathogenicity diarrhea co-infection |
| title | Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China |
| title_full | Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China |
| title_fullStr | Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China |
| title_full_unstemmed | Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China |
| title_short | Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China |
| title_sort | isolation and preliminary pathogenicity of a recent feline astrovirus strain in china |
| topic | FeAstV isolation pathogenicity diarrhea co-infection |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1614686/full |
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