RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System
The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification...
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MDPI AG
2022-05-01
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| author | Yuxing Shi Peng Ye Chuang Wang Kuojun Yang Jinhong Guo |
| author_facet | Yuxing Shi Peng Ye Chuang Wang Kuojun Yang Jinhong Guo |
| author_sort | Yuxing Shi |
| collection | DOAJ |
| description | The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification for such chips is necessary to improve the rapidity and sensitivity of the system. Chemical modification is commonly used, but there is little research on the hydrophilic effect of different concentrations of hydrophilic reagents. According to function requirements for different microchannels of the chip (some only need to ensure the liquid can flow into the next chamber, and some also need to ensure the function of “closing the door” during immunoassay incubation), we explored the best combination of hydrophilic reagent and concentration through experiments. Firstly, three hydrophilic reagents were used for modification. Secondly, the hydrophilic effects of different reagents and concentrations were explored by contact angle test, the influence of different modification methods on liquid transfer efficiency was characterized by residual liquid calculation in the chamber. Finally, the effect of different hydrophilic reagents on absorbance was also tested. By experimental results and comprehensively considering the stability of the modification effect and the function requirements, Tween-20 (2.0% <i>v</i>/<i>v</i>) was chosen as the modifying reagents of the first siphon valve and the second siphon valve, and TritonX-100 (2.0% <i>v</i>/<i>v</i>) was chosen for the third siphon valve, which effectively reduces the contact angle and improves the liquid transfer efficiency, leading to further improvement of the rapidity and sensitivity of the centrifugal microfluidic immunoassay system by efficient siphoning and high plasma separation efficiency (99%). |
| format | Article |
| id | doaj-art-6f889bba2858447dbf06b7ed9fb53b11 |
| institution | OA Journals |
| issn | 2072-666X |
| language | English |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
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| series | Micromachines |
| spelling | doaj-art-6f889bba2858447dbf06b7ed9fb53b112025-08-20T01:53:36ZengMDPI AGMicromachines2072-666X2022-05-0113683110.3390/mi13060831RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay SystemYuxing Shi0Peng Ye1Chuang Wang2Kuojun Yang3Jinhong Guo4School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 611731, ChinaSchool of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 611731, ChinaSchool of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 611731, ChinaSchool of Automation Engineering, University of Electronic Science and Technology of China, Chengdu 611731, ChinaThe M.O.E. Key Laboratory of Laboratory Medical Diagnostics, The College of Laboratory Medicine, Chongqing Medical University, No. 1 Yixueyuan Road, Chongqing 400016, ChinaThe surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification for such chips is necessary to improve the rapidity and sensitivity of the system. Chemical modification is commonly used, but there is little research on the hydrophilic effect of different concentrations of hydrophilic reagents. According to function requirements for different microchannels of the chip (some only need to ensure the liquid can flow into the next chamber, and some also need to ensure the function of “closing the door” during immunoassay incubation), we explored the best combination of hydrophilic reagent and concentration through experiments. Firstly, three hydrophilic reagents were used for modification. Secondly, the hydrophilic effects of different reagents and concentrations were explored by contact angle test, the influence of different modification methods on liquid transfer efficiency was characterized by residual liquid calculation in the chamber. Finally, the effect of different hydrophilic reagents on absorbance was also tested. By experimental results and comprehensively considering the stability of the modification effect and the function requirements, Tween-20 (2.0% <i>v</i>/<i>v</i>) was chosen as the modifying reagents of the first siphon valve and the second siphon valve, and TritonX-100 (2.0% <i>v</i>/<i>v</i>) was chosen for the third siphon valve, which effectively reduces the contact angle and improves the liquid transfer efficiency, leading to further improvement of the rapidity and sensitivity of the centrifugal microfluidic immunoassay system by efficient siphoning and high plasma separation efficiency (99%).https://www.mdpi.com/2072-666X/13/6/831hydrophilic modificationcontact angleliquid transfer efficiencycentrifugal microfluidic immunoassay system |
| spellingShingle | Yuxing Shi Peng Ye Chuang Wang Kuojun Yang Jinhong Guo RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System Micromachines hydrophilic modification contact angle liquid transfer efficiency centrifugal microfluidic immunoassay system |
| title | RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System |
| title_full | RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System |
| title_fullStr | RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System |
| title_full_unstemmed | RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System |
| title_short | RETRACTED: Surface Hydrophilic Modification for Chip of Centrifugal Microfluidic Immunoassay System |
| title_sort | retracted surface hydrophilic modification for chip of centrifugal microfluidic immunoassay system |
| topic | hydrophilic modification contact angle liquid transfer efficiency centrifugal microfluidic immunoassay system |
| url | https://www.mdpi.com/2072-666X/13/6/831 |
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