Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation

Objective. The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system. Background Data. Treatment with light in the spectrum from re...

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Main Authors: Shuang Li, Qiang-Li Wang, Xin Chen, Xian-qiang Mi
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:International Journal of Photoenergy
Online Access:http://dx.doi.org/10.1155/2014/971491
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author Shuang Li
Qiang-Li Wang
Xin Chen
Xian-qiang Mi
author_facet Shuang Li
Qiang-Li Wang
Xin Chen
Xian-qiang Mi
author_sort Shuang Li
collection DOAJ
description Objective. The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system. Background Data. Treatment with light in the spectrum from red to near-infrared region has beneficial effect on tissue injury and 670 nm LED is currently under clinical investigation for retinoprotective therapy. Methods. Studies were conducted in the cultured cells under hypoxia treated by cobalt chloride (CoCl2). After irradiation by 670 nm LED with different power densities, cell viability, cytochrome C oxidase activity, and ATP concentration were measured. Results. The irradiation of 670 nm LED significantly improved cell viability, cytochrome C oxidase activity, and ATP concentration in the hypoxia RF/6A cells. Conclusion. 670 nm LED irradiation could recover the hypoxia damage caused by CoCl2. Photobiomodulation of 670 nm LED plays a potential role for the treatment of diabetic retinopathy.
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institution Kabale University
issn 1110-662X
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language English
publishDate 2014-01-01
publisher Wiley
record_format Article
series International Journal of Photoenergy
spelling doaj-art-6f80f1ce2aed4e4db5f045f4e1f868cf2025-08-20T03:34:05ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/971491971491Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode IrradiationShuang Li0Qiang-Li Wang1Xin Chen2Xian-qiang Mi3College of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaCollege of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaCollege of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaObjective. The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system. Background Data. Treatment with light in the spectrum from red to near-infrared region has beneficial effect on tissue injury and 670 nm LED is currently under clinical investigation for retinoprotective therapy. Methods. Studies were conducted in the cultured cells under hypoxia treated by cobalt chloride (CoCl2). After irradiation by 670 nm LED with different power densities, cell viability, cytochrome C oxidase activity, and ATP concentration were measured. Results. The irradiation of 670 nm LED significantly improved cell viability, cytochrome C oxidase activity, and ATP concentration in the hypoxia RF/6A cells. Conclusion. 670 nm LED irradiation could recover the hypoxia damage caused by CoCl2. Photobiomodulation of 670 nm LED plays a potential role for the treatment of diabetic retinopathy.http://dx.doi.org/10.1155/2014/971491
spellingShingle Shuang Li
Qiang-Li Wang
Xin Chen
Xian-qiang Mi
Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation
International Journal of Photoenergy
title Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation
title_full Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation
title_fullStr Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation
title_full_unstemmed Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation
title_short Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation
title_sort photobiomodulation for cobalt chloride induced hypoxic damage of rf 6a cells by 670 nm light emitting diode irradiation
url http://dx.doi.org/10.1155/2014/971491
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AT xianqiangmi photobiomodulationforcobaltchlorideinducedhypoxicdamageofrf6acellsby670nmlightemittingdiodeirradiation